Strains

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-delta 760-979
Background strain: 40D4
Plasmid transformation: HAP2-delta 760-979 plasmid with aphVIII in one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA can rescue mfus5
Background strain: B215
Plasmid transformation: pSL72 (aphVIII) inserted

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-K368R rescued 40D4
Background strain: 40D4
Plasmid transformation: HAP2-K368R plasmid rescued 40D4, one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-deltaTRA in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-deltaTRA(184-6) plasmid transformed 40D4, one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: Hsp-HAP2-HA transformed gam10
Background strain: gam10
Plasmid transformation: Len2 (Hsp-HAP2-HA) plasmid transformed gam10, co-transformation

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA transformed gam10
Background strain: gam10
Plasmid transformation: HAP2-HA (pYJ58) transformed gam10, co-transformation

Liu Y, Misamore MJ, Snell WJ (2010) Membrane fusion triggers rapid degradation of two gamete-specific, fusion-essential proteins in a membrane block to polygamy in Chlamydomonas. Development. 137:1473-81

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2HA-760 transformed 40D4
Background strain: 40D4
Plasmid transformation: HAP2HA-760 plasmid into 40D4, one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: hap2 mutant
Background strain: B215
Plasmid transformation: NIT plasmid (pMN56) transformed into B215

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: hap2 mutant
Background strain: 21gr
Plasmid transformation: Obtained by cross with HAP2-HA minus cells

Liu Y, Tewari R, Ning J, Blagborough AM, Garbom S, Pei J, Grishin NV, Steele RE, Sinden RE, Snell WJ, Billker O (2008) The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes Dev. 22:1051-68

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA and MAR1-Flag in hap2 cell
Background strain: Hap2 (63B10)
Plasmid transformation: MAR1-Flag-Ble plasmid transformed into HAP2-HA cell (Liu, 2008)

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2 C-terminal deleted until 1054
Background strain: 40D4
Plasmid transformation: HAP2-1054-aphVIII plasmid in hap2 cell, one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-C366A in 40D4 cell
Background strain: 40D4
Plasmid transformation: HAP2-C366A-aphVIII in hap2 cell

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HSV-Flag plasmid in gam-10 cells
Background strain: gam10
Plasmid transformation: HAP2-HSV-Flag-ble plasmid transformed gam-10 cell, one plasmid transformation

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-L391GQ394P mutation in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-L391GQ394P-aphVIII transformed 40D4 cell, one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-CC/SS cells transformed with MAR1-Flag plasmid
Background strain: HAP2-CC/SS
Plasmid transformation: MAR1-Flag-Ble plasmid transformed into HAP2-CC/SS cell, one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: MAR1-Flag knock-down cell
Background strain: 40D4
Plasmid transformation: MAR1 knock-down plasmid (hygromycin) transformed into hap2/FUS1R

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-K368M mutation
Background strain: 40D4
Plasmid transformation: HAP2-K368M-aphVIII plasmid transformed into 40D4 cell

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-R185K mutation in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-R185K-aphVIII plasmid transformed into 40D4

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-RA185-6AR in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-RA185-6AR-aphVIII plasmid transformed into 40D4

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-D367A in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-D367A-aphVIII plasmid transformed into 40D4

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA plasmid in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-HA-aphVIII plasmid transformed into 40D4

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-Myc-HSV plasmid in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-Myc-TEV-HSV-aphVIII plasmid transformed into 40D4, one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-Flag plasmid transformed into HAP2-HA cell (pYJ58, liu-2008)
Background strain: 63B10
Plasmid transformation: hap2::HAP2- HA plasmid transformed into HAP2-HA cell (pYJ58, liu-2008), one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-deltaDKVG (367-70) in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-deltaDKVG-aphVIII plasmid transformed into 40D4, one plasmid

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-K368A in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-K368A-aphVIII plasmid transformed into 40D4

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2 ectodomain only in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-ecto-aphVIII plasmid transformed into 40D4

Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-A668K in 40D4, modify for easy mass spect, detect palmitoylation
Background strain: 40D4
Plasmid transformation: HAP2-A668K-aphVIII plasmid transformed into 40D4, one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA-GFP in 63B10
Background strain: 63B10
Plasmid transformation: HAP2-HA-GFP plasmid transformed into 63B10, co-transformation (NIT)

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: CrHAP2 fusion loop replaced with Dengue FL
Background strain: 40D4
Plasmid transformation: HAP2-HA-APEX -aphVIII plasmid transformed into 40D4

  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Yanjie Liu, William Snell lab, UT Southwestern Medical Center, May 2016

Mutant description: HAP2-HA-APEX in 40D4
Background strain: 40D4
Plasmid transformation: HAP2-HA-APEX-aphVIII plasmid transformed into 40D4, one plasmid

  • Locus:
  • HAP2
  • Chromosome:
  • 16