Strains
CC-5338 Osmo26 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
Phenotype: short CV cycle, smaller CVs
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5339 Osmo33 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5340 Osmo38 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
Phenotype: bigger CVs
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5341 Osmo40 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5342 Osmo41 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5343 Osmo43 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
Phenotype: bigger CVs, longer CV cycle
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM su
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5344 Osmo45 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5345 Osmo47 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
Phenotype: bigger CVs
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5346 Osmo50 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5347 Osmo56 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5348 Osmo61 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5349 Osmo72 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
CC-5350 Osmo73 [Becker]
$30.00
$30.00
From Karin Komsic-Buchmann, Becker lab, University of Cologne, October 2016
Phenotype: longer CV cycle, smaller CVs
This strain cannot survive under osmotic conditions below 140 mosM.
Culture maintenance: photon flux ~20-70 mmol/m²s,14/10 hours light/dark cycle, 21°C, TAP + 0.2 g/l arginine + 64.6 mM sucrose
Komsic-Buchmann K, Stephan LM, Becker B (2012) The SEC6 protein is required for contractile vacuole function in Chlamydomonas reinhardtii. J Cell Sci 125:2885–2895
Komsic-Buchmann, Karin (2014) Structure and Function of the Contractile Vacuoles of Chlamdomonas reinhardtii. Dr. Hut, Munich, Germany.
From Lynne Quarmby, Simon Fraser University, March 2017
Phenotype: impaired flagellar autotomy
Hilton LK, Meili F, Buckoll PD, Rodriguez-Pike JC, Choutka CP, Kirschner JA, Warner F, Lethan M, Garces FA, Qi J, Quarmby LM (2016) A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel. G3 (Bethesda). 6:3409-3418
CC-5356 adf3-2 mt+ [E21 C7]
$30.00
$30.00
From Lynne Quarmby, Simon Fraser University, March 2017
Phenotype: impaired flagellar autotomy
Hilton LK, Meili F, Buckoll PD, Rodriguez-Pike JC, Choutka CP, Kirschner JA, Warner F, Lethan M, Garces FA, Qi J, Quarmby LM (2016) A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel. G3 (Bethesda). 6:3409-3418
CC-5357 RbcS1-Venus mt-
$30.00
$30.00
From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017
Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63
CC-5358 RCA1-Venus mt-
$30.00
$30.00
From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017
Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63
CC-5359 EPYC1-Venus mt-
$30.00
$30.00
From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017
Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63
CC-5360 epyc1 mt-
$30.00
$30.00
From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017
This mutant should be maintained in low light (~50 uE or less) or TAP in the dark.
Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63
From Liz Freeman Rosenzweig, but created by Luke Mackinder in the Jonikas lab at Carnegie, May 2017
Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016) A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle. Proc Natl Acad Sci U S A. 113:5958-63
CC-5362 EV2 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5363 EV3 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5364 EV4 mt+
$30.00
Read More
$30.00 Read More
From Steven King, University of Connecticut Health Center, June 2017
This is a flagellated empty vector control strain used in analysis of amiRNA strains that reduce levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850).
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5365 PAM amiRNA1 #5 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a weak knockdown of PAM expression resulting in a mixture of flagellated and unflagellated cells. For a strong knockdown of PAM expression use PAM amiRNA2 #3 or #8
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5366 PAM amiRNA1 #6 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a weak knockdown of PAM expression resulting in a mixture of flagellated and unflagellated cells. For a strong knockdown of PAM expression use PAM amiRNA2 #3 or #8
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5367 PAM amiRNA2 #3 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a strong knockdown of PAM expression to ~30% of control levels resulting in aflagellate cells.
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5368 PAM amiRNA2 #8 mt+
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This is an amiRNA strain with reduced levels of the enzyme peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). This strain exhibits a strong knockdown of PAM expression to ~10% of control levels resulting in aflagellate cells.
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
CC-5369 CrPAM-ΔCD
$30.00
$30.00
From Steven King, University of Connecticut Health Center, June 2017
This CLiP library strain contains an insertion in the gene for peptidylglycine alpha-amidating monooxygenase (PAM; Cre03.g152850). The insertion occurs in the region encoding the cytosolic C-terminal domain, and results in deletion of the last 75 residues and incorporation of an 18-residue sequence derived from the insertion cassette. This strain exhibits enhanced PAM enzyme activity.
Kumar D, Blaby-Haas CE, Merchant SS, Mains RE, King SM, Eipper BA (2016) Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity. J Cell Sci 129:943-56
Kumar D, Strenkert D, Patel-King RS, Leonard MT, Merchant SS, Mains RE, King SM, Eipper BA (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 10.7554/eLife.25728
From Martin Spalding, Iowa State University, June 2017
21gr (CC-1690) was initially crossed to CC-1691 to obtain a mt- strain, and then backcrossed to 21gr (CC-1690) fifteen times. This is an mt- strain from the final backcross.
CC-5371 sta6.2 mt-
$30.00
$30.00
From Martin Spalding, Iowa State University, June 2017
This starchless mutant arose from a TALEN targeted mutagenesis experiment using 21gr (CC-1690). The mutation in the STA6 gene is a single base deletion at the intron 3/exon 4 border yielding the following sequence: GATGCAGaCGGTGCT where the lowercase ‘a’ is the deleted base. To obtain a minus mating type, the initial mutant was crossed to CC-1691. A starchless mt- progeny lacking the TALEN transgene was subsequently backcrossed to 21gr (CC-1690) thirteen times. This strain is a mt- progeny from the final cross.
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