Strains

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10; ARG7

This is the amc5 strain complemented with the wild-type NUOB10 gene.

Mutagenesis of amc5 with cosmid 7D10 (Rochaix cosmid library) containing the wild-type NUOB10 gene and selected based on ARG7 marker in the cosmid. It is paromomycin resistant and an arginine prototroph.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10-FLAG; APHVII

This is the amc5 strain complemented with the wild-type NUOB10-FLAG gene.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the wild-type NUOB10 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C79S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C79S substitution.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the wild-type NUOB10 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C91S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C91S substitution.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the NUOB10 gene encoding the C91S substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C79S,C91S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C79S and C91S substitution.

Mutagenesis of amc5 with plasmid pRS426 containing the NUOB10 gene encoding the double C79S and C91S substitutions with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

  • Locus:
  • Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.
  • Chromosome:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; amc1-1; APHVII

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is mitochondrial complex I-deficient. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. APHVII transgene present in an unknown location. This strain in hygromycin resistant.

It was created by mutagenesis of 3A+ with a cassette containing the APHVII transgene from pHyg3.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-1

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is mitochondrial complex I-deficient. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. It is a cross of amc1(4C10) with CC-124 resulting in meiotic progeny that is arg+ and hygromycin sensitive.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin sensitive and an arginine prototroph.

Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-1

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is a cross of amc1(4C10) with 4C- resulting in meiotic progeny that is arg- and hygromycin sensitive. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. APHVII transgene present in an unknown location. Originally isolated as a mitochondrial complex I-deficient strain but now the phenotype has reverted.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin sensitive.

Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc8

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin resistant.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts exon 2 of NUO5 gene Cre10.g450400. It is a mitochondrial complex I-deficient strain and is hygromycin resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5; ARG7

This is the amc9 strain complemented with the wild-type Cre10.g450400 gene. It was created by mutagenesis of amc9 with cosmid 9A2 (Rochaix cosmid library) containing the full-length wild-type NUO5 (Cre10.g450400) and selected based on ARG7 marker in the cosmid. This strain is hygromycin resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5-FLAG; APHVIII

This is the amc9 strain complemented with the wild-type NUO5 gene with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the wild-type NUO5 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5K230R-FLAG; APHVIII

This is the amc9 strain complemented with the NUO5 gene containing the K230R substitution with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the NUO5 gene encoding the K230R substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc10

This strain was originally a mitochondrial complex I-deficient strain that reverted in its phenotype. It was originally created by mutagenesis of 4C- with a cassette containing APHVII transgene from pHyg3. It is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; APHVII; amc10

This strain is meiotic progeny from a cross of amc10 (42A10) with CC-124. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc10

This strain is meiotic progeny from a cross of amc10 (42A10) with CC-124. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2;APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

  • Locus:
  • Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.<br><br><br>Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with cosmid 1H10 (Rochaix cosmid library) containing the full-length wild-type AMC11 (Cre16.g688900) and CYG56 (Cre16.g688901) genes and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).

  • Locus:
  • Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

Yes

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with truncated cosmid 1H10 (Rochaix cosmid library) containing the wild-type AMC11 (Cre16.g688900) gene and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).

  • Locus:
  • Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVI; amc13

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc13

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B sensitive and an arginine auxotroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; amc1-2; APHVII

This is a mitochondrial complex I-deficient strain. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Heide Evers, Hegemann lab, Humboldt University-Berlin, January 2020

Strain PH239 ∆COP1-F6 includes the 581 bp insert inside COP1

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.

  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.

  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.

  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

DNA insertional mutant CAL028_03_28 from the CAL collection which is defective in the RBD1 gene (Cre07.g315150).

Maintain on TAP plates containing 25 μg/mL paromomycin in dark or very low light.

Calderon RH, Garc’a-Cerd‡n JG, Malno‘ A, Cook R, Russell JJ, Gaw C, Dent RM, de Vitry C, Niyogi KK (2013) A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs. J Biol Chem. 288:26688-96

García-Cerdán JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640

  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with WT RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of WT RBD1.

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.

Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640

  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with mutant RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of C76/109G mutant version of RBD1.

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.

Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640

  • Locus:
  • RBD1
  • Chromosome:
  • 7