From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation W173A/F177A transformed into 40d4 strain.


Feng J, Dong X, Pinello J, Zhang J, Lu C, Iacob RE, Engen JR, Snell WJ, Springer TA. Fusion surface structure, function, and dynamics of gamete fusogen HAP2. Elife. 2018 Oct 3;7:e39772. doi: 10.7554/eLife.39772. PMID: 30281023; PMCID: PMC6170185.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation F192A/W193A transformed into 40d4 strain.


Feng J, Dong X, Pinello J, Zhang J, Lu C, Iacob RE, Engen JR, Snell WJ, Springer TA. Fusion surface structure, function, and dynamics of gamete fusogen HAP2. Elife. 2018 Oct 3;7:e39772. doi: 10.7554/eLife.39772. PMID: 30281023; PMCID: PMC6170185.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation L197A/L200A/I201A transformed into 40d4 strain.


Feng J, Dong X, Pinello J, Zhang J, Lu C, Iacob RE, Engen JR, Snell WJ, Springer TA. Fusion surface structure, function, and dynamics of gamete fusogen HAP2. Elife. 2018 Oct 3;7:e39772. doi: 10.7554/eLife.39772. PMID: 30281023; PMCID: PMC6170185.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation W173A/F177A/ F192A/W193A transformed into 40d4 strain.


Zhang J, Pinello JF, Fernández I, Baquero E, Fedry J, Rey FA, Snell WJ. Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2. Nat Commun. 2021 Jul 19;12(1):4380. doi: 10.1038/s41467-021-24613-8. PMID: 34282138; PMCID: PMC8289870.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation W173A/F177A/ F192A/W193A /L197A/L200A/I201A transformed into 40d4 strain.


Zhang J, Pinello JF, Fernández I, Baquero E, Fedry J, Rey FA, Snell WJ. Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2. Nat Commun. 2021 Jul 19;12(1):4380. doi: 10.1038/s41467-021-24613-8. PMID: 34282138; PMCID: PMC8289870.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation W173C transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation E126L transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation E126A transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation S909A transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation S826A transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation H209A transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Jun Zhang, William Snell, University of Maryland, College Park, August 2021

HA tagged HAP2 with point mutation S826D transformed into 40d4 strain.


  • Locus:
  • HAP2
  • Chromosome:
  • 16

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.


  • Locus:
  • TUB2
  • Chromosome:
  • 12

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.


  • Locus:
  • TUB2
  • Chromosome:
  • 12

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.


  • Locus:
  • TUA1, TUB1
  • Chromosome:
  • 3, 12

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.


  • Locus:
  • TUA1, TUB1
  • Chromosome:
  • 3,12

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.


  • Locus:
  • TUA2, TUB1
  • Chromosome:
  • 4, 12

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.

From Takako Kato-Minoura, Chuo University-Japan, October 2021


Kato-Minoura T, Ogiwara Y, Yamano T, Fukuzawa H, Kamiya R. Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. PLoS One. 2020 Nov 23;15(11):e0242694. doi: 10.1371/journal.pone.0242694. PMID: 33227038; PMCID: PMC7682851.