Strains
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-1691 Sager y1 6145 mt- used by the Chlamydomonas Pan-Genome project.
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-2342 wild type mt- Jarvik #6, Pittsburgh, PA used by the Chlamydomonas Pan-Genome project.
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-2343 wild type mt+ Jarvik #224, Melbourne, FL used by the Chlamydomonas Pan-Genome project.
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-2936 C. reinhardtii, Quebec, LEE-2 mt+ used by the Chlamydomonas Pan-Genome project.
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-2937 C. reinhardtii, Quebec, LEE-3 mt+ used by the Chlamydomonas Pan-Genome project.
From Rory Craig, University of California-Berkeley, July 2023
This Japanese strain is from a single clone of NIES-2463, from the MCC NIES culture collection in Japan. It was collected in Kagoshima, Japan in 2006. This isolate was used by the Chlamydomonas Pan-Genome project.
Nakada T, Shinkawa H, Ito T, Tomita M. Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. J Plant Res. 2010 Jan;123(1):67-78. doi: 10.1007/s10265-009-0266-0. Epub 2009 Oct 31. PMID: 19882207.
From Rory Craig, University of California-Berkeley, July 2023
This strain was isolated by the Rob Ness laboratory at Toronto University in 2022. This is a field isolate from Toronto, Canada. It is interfertile with the laboratory strains of Chlamydomonas reinhardtii. This isolate was used by the Chlamydomonas Pan-Genome project.
Ford SA, Craig RJ, Ness RW. A novel method for identifying Chlamydomonas reinhardtii (Chlorophyta) and closely related species from nature. J Phycol. 2023 Feb;59(1):281-288. doi: 10.1111/jpy.13306. Epub 2022 Dec 21. PMID: 36453860.
From Susan Dutcher, Washington University in St. Louis, March 2022
Single colony isolate of CC-5695 C. reinhardtii, wild type mt+ [SD10] used by the Chlamydomonas Pan-Genome project.
From Pete Lefebvre, University of Minnesota, March 2022
Wild-type strain collected by Pete Lefebvre in Ile des Embiez, France in May 2021 from salty, dry, rocky soil.. This strain has short paralyzed flagella and does not swim.
From Pete Lefebvre, University of Minnesota, March 2022
Wild-type strain collected by Pete Lefebvre in Ile des Embiez, France in May 2021 from salty, dry, rocky soil. This strain swims in contrast to CC-5830 Embiez 1C, which does not.
CC-5852 lcla1 mt+
$30.00
$30.00
From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022
Alias: lcla1#PH25-6
Genotype: mt+; Cre07.g329861::APHVII
Background: CC-4533/CC-5155
Origin: lcla1 [arg+] spore obtained from 4th backcross of amc12#6E9 to CC-4533/CC-5155. The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.
CC-5853 lcla1(EV) mt- *Dim*
$30.00
$30.00
From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022
Alias: lcla1#PH25-1 (pCB412)#3
Genotype: mt-; Cre07.g329861::APHVII; ARG7
Background: CC-4533/CC-5155
Origin: lcla1 [arg+] empty vector (EV) isolated transformant. lcla1#PH25-1 (an arg7-8 spore obtained from the same cross as lcla1#PH25-6, that is, the 4th backcross of amc12#6E9 to CC-4533/CC-5155) transformed with the pCB412 vector used in generation of the Chlamydomonas cosmid library. The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.
CC-5854 lcla1(LCLA1) mt-
$30.00
$30.00
From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022
Alias: lcla1#PH25-1 (pADC2)#19
Genotype: mt-; Cre07.g329861::APHVII; LCLA1::ARG7
Background: CC-4533/CC-5155
Origin: lcla1#PH25-1 complemented strain transformed with pADC2 (64B9 cosmid further restricted). lcla1#PH25-1 (an arg7-8 spore obtained from the same cross as lcla1#PH25-6, that is, the 4th backcross of amc12#6E9 to CC-4533/CC-5155). The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.
From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022
Alias: lcla1#PH25-1 (pADC10)#25
Genotype: mt-; Cre07.g329861::APHVII; LCLA1-3xFLAG::ARG7
Background: CC-4533/CC-5155
Origin: lcla1#PH25-1 complemented strain, transformed with pADC10 (pADC2 with sequence encoding 3xFLAG epitope inserted at 3’ end of gene) for detection of LCLA1-3xFLAG. lcla1#PH25-1 (an arg7-8 spore obtained from the same cross as lcla1#PH25-6, that is, the 4th backcross of amc12#6E9 to CC-4533/CC-5155). The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.
From Andrew Castonguay, Patrice Hamel Lab, The Ohio State University, March 2022
Alias: lcla1#PH25-1 (pADC11)#42
Genotype: mt-; Cre07.g329861::APHVII; LCLA1-6xFLAG::ARG7
Background: CC-4533/CC-5155
Origin: lcla1#PH25-1 complemented strain, transformed with pADC11 (pADC2 with tandem insertions of sequence encoding 3xFLAG epitope) for detection of LCLA1-6xFLAG. lcla1#PH25-1 (an arg7-8 spore obtained from the same cross as lcla1#PH25-6, that is, the 4th backcross of amc12#6E9 to CC-4533/CC-5155). The amc12#6E9 original mutant was obtained through insertional mutagenesis described in Subrahmanian, et al., 2020.
Culture maintenance: low light (< 50 µE) on selective media, TAP+HygB
Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. PMID: 32025618; PMCID: PMC6996877.
CC-5857 oda6::IC2-NG mt-
$30.00
$30.00
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was generated by transformation of oda6 with IC2-NG and selected for rescued motility. The essential ODA subunit IC2 is tagged with mNeonGreen resulting in brightly fluorescent cilia.
Dai J, Barbieri F, Mitchell DR, Lechtreck KF. In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties. Mol Biol Cell. 2018 Oct 15;29(21):2553-2565. doi: 10.1091/mbc.E18-05-0291. Epub 2018 Aug 22. PMID: 30133350; PMCID: PMC6254574.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating oda3 oda6::IC2-NG and ift20::IFT20-mCherry. The outer dynein arms (ODAs) are tagged on the essential IC2 subunits with mNeonGreen and IFT is visible by IFT20-mCheery expression. ODAs do not assemble in cilia due to the absence of the docking complex in oda3. This allows imaging of ODA transport by IFT.
Dai J, Barbieri F, Mitchell DR, Lechtreck KF. In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties. Mol Biol Cell. 2018 Oct 15;29(21):2553-2565. doi: 10.1091/mbc.E18-05-0291. Epub 2018 Aug 22. PMID: 30133350; PMCID: PMC6254574.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating armc2::ARMC2-mScarlet and ida3::IDA3-mNG. It expresses ARMC2-mScarlet, a cargo adapter for IFT of radial spokes, and IDA3-mNG, a cargo adapter for IFT of IDA I1/f, in a corresponding double mutant back ground. Very dim in full-length flagella but stronger during ciliary assembly.
Lechtreck KF, Liu Y, Dai J, Alkhofash RA, Butler J, Alford L, Yang P. Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes. Elife. 2022 Jan 4;11:e74993. doi: 10.7554/eLife.74993. PMID: 34982025; PMCID: PMC8789290.
Hunter EL, Lechtreck K, Fu G, Hwang J, Lin H, Gokhale A, Alford LM, Lewis B, Yamamoto R, Kamiya R, Yang F, Nicastro D, Dutcher SK, Wirschell M, Sale WS. The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length. Mol Biol Cell. 2018 Apr 15;29(8):886-896. doi: 10.1091/mbc.E17-12-0729. Epub 2018 Mar 29. PMID: 29467251; PMCID: PMC5896928.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
The ift54-2 mS-IFT54 strain allows visualization of IFT with a bright red fluorescence, e.g., in combination with GFP-tagged proteins.
Wingfield JL, Mengoni I, Bomberger H, Jiang YY, Walsh JD, Brown JM, Picariello T, Cochran DA, Zhu B, Pan J, Eggenschwiler J, Gaertig J, Witman GB, Kner P, Lechtreck K. IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife. 2017 May 31;6:e26609. doi: 10.7554/eLife.26609. PMID: 28562242; PMCID: PMC5451262.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
The strain allows imaging of IFT via the very bright IFT54-mNG signal.
Wingfield JL, Mengoni I, Bomberger H, Jiang YY, Walsh JD, Brown JM, Picariello T, Cochran DA, Zhu B, Pan J, Eggenschwiler J, Gaertig J, Witman GB, Kner P, Lechtreck K. IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife. 2017 May 31;6:e26609. doi: 10.7554/eLife.26609. PMID: 28562242; PMCID: PMC5451262.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by mating ift140-1::IFT140-sfGFP and ift54-2::mScarlet-IFT54. It facilitates simultaneous 2-color imaging of the IFT-A and IFT-B subcomplexes.
Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB. A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J Cell Sci. 2019 Feb 11;132(3):jcs220749. doi: 10.1242/jcs.220749. PMID: 30659111; PMCID: PMC6382014.
Wingfield JL, Mengoni I, Bomberger H, Jiang YY, Walsh JD, Brown JM, Picariello T, Cochran DA, Zhu B, Pan J, Eggenschwiler J, Gaertig J, Witman GB, Kner P, Lechtreck K. IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium. Elife. 2017 May 31;6:e26609. doi: 10.7554/eLife.26609. PMID: 28562242; PMCID: PMC5451262.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by transforming ift81-1 with pBR25-mNF-IFT82. It allows imaging of IFT via the very bright IFT81-mNG signal.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644.
Kubo T, Brown JM, Bellve K, Craige B, Craft JM, Fogarty K, Lechtreck KF, Witman GB. Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. J Cell Sci. 2016 May 15;129(10):2106-19. doi: 10.1242/jcs.187120. Epub 2016 Apr 11. PMID: 27068536; PMCID: PMC5506485.
From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2022
This strain was obtained by transforming ift74-1 with pBR25-mS-IFT74.
The ift74-1 mS-IFT74 strain allows visualization of IFT with a bright red fluorescence, e.g., in combination with GFP-tagged proteins.
Wingfield JL, Mekonnen B, Mengoni I, Liu P, Jordan M, Diener D, Pigino G, Lechtreck K. In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip. J Cell Sci. 2021 Sep 15;134(18):jcs259010. doi: 10.1242/jcs.259010. Epub 2021 Sep 23. PMID: 34415027; PMCID: PMC8487644
CC-5866 gun4-II [6F14]
$30.00
$30.00
From Mautusi Mitra, University of West Georgia, March 2022
Background: 4A+
Origin: random insertional mutagenesis using the linearized pBC1 plasmid which contains the AphVIII gene that confers resistance against paromomycin; pBC1 plasmid was obtained from Dr. Kris Niyogi (UC Berkeley); strain is paromomycin resistant; for details please see published research article
Culture maintenance: strain can be maintained under dim light (15-25 µmole m-2s-1); can be maintained on TAP or on TAP + Paromomycin plates containing 10 – 15 µg of Paromomycin/mL of TAP medium.
Comment: progressively photo-bleaches with increase in light intensity; see published article for more detail.
Grovenstein PB, Wilson DA, Lankford KD, Gaston KA, Perera S, Mitra M. Identification and molecular characterization of the second Chlamydomonas gun4 mutant, gun4-II. F1000Res. 2013 Jun 19;2:142. doi: 10.12688/f1000research.2-142.v2. PMID: 24627785; PMCID: PMC3931455.
CC-5869 Hyd A1/A2 flvB308-1
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: Hydrogenase and FlvB mutant, mt-; background CC-4533
Gene mutated: Cre16.g691800; Cre09.g396600.t1.1; Cre03.g199800.t1.1
Antibiotic resistance: Paromomycin; Hygromycin
Reference strain: HydA1/A2 and flvB308
Burlacot A, Sawyer A, Cuiné S, Auroy-Tarrago P, Blangy S, Happe T, Peltier G. Flavodiiron-Mediated O2 Photoreduction Links H2 Production with CO2 Fixation during the Anaerobic Induction of Photosynthesis. Plant Physiol. 2018 Aug;177(4):1639-1649. doi: 10.1104/pp.18.00721. Epub 2018 Jul 5. PMID: 29976836; PMCID: PMC6084654.
CC-5870 Hyd A1/A2 flvB308-3
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: Hydrogenase and FlvB mutant, mt-; background CC-4533
Gene mutated: Cre16.g691800; Cre09.g396600.t1.1; Cre03.g199800.t1.1
Antibiotic resistance: Paromomycin; Hygromycin
Reference strain: HydA1/A2 and flvB308
Burlacot A, Sawyer A, Cuiné S, Auroy-Tarrago P, Blangy S, Happe T, Peltier G. Flavodiiron-Mediated O2 Photoreduction Links H2 Production with CO2 Fixation during the Anaerobic Induction of Photosynthesis. Plant Physiol. 2018 Aug;177(4):1639-1649. doi: 10.1104/pp.18.00721. Epub 2018 Jul 5. PMID: 29976836; PMCID: PMC6084654.
CC-5871 FlvB21 mt+ (1)
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: flavodiiron protein B mutant, nit-, mt+; parental strains flvB 21 (LMJ.RY0402,214714) and CC-5155
Gene mutated: Cre16.g691800
Antibiotic resistance: Paromomycin
Sensitivity: short-term light fluctuations
Reference strain: CC-5155 and CC-4533
Burlacot A, Richaud P, Gosset A, Li-Beisson Y, Peltier G. Algal photosynthesis converts nitric oxide into nitrous oxide. Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2704-2709. doi: 10.1073/pnas.1915276117. Epub 2020 Jan 15. PMID: 31941711; PMCID: PMC7007583.
CC-5872 FlvB21 mt+ (2)
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: flavodiiron protein B mutant, nit-, mt+; parental strains flvB 21 (LMJ.RY0402,214714) and CC-5155
Gene mutated: Cre16.g691800
Antibiotic resistance: Paromomycin
Sensitivity: short-term light fluctuations
Reference strain: CC-5155 and CC-4533
Burlacot A, Dao O, Auroy P, Cuiné S, Li-Beisson Y, Peltier G. Alternative electron pathways of photosynthesis drive the algal CO2 concentrating mechanism. bioRxiv 2021.02.25.432959; doi: https://doi.org/10.1101/2021.02.25.432959
CC-5873 FlvB CYP-1
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: flvB pgrl1 double mutant; parental strains flvB 21 mt+1 and pgrl1
Gene mutated: Cre16.g691800 ; Cre01.g007950.t1.1
Antibiotic resistance: Paromomycin
Sensitivity: short-term light fluctuations
Reference strain: CC-5155 and CC-4533
Burlacot A, Richaud P, Gosset A, Li-Beisson Y, Peltier G. Algal photosynthesis converts nitric oxide into nitrous oxide. Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2704-2709. doi: 10.1073/pnas.1915276117. Epub 2020 Jan 15. PMID: 31941711; PMCID: PMC7007583.
CC-5874 FlvB CYP-2
$30.00
$30.00
From Adrien Burlacot, Carnegie Institution for Science, March 2022
Background and origin: flvB pgrl1 double mutant; parental strains flvB 21 mt+1 and pgrl1
Gene mutated: Cre16.g691800 ; Cre01.g007950.t1.1
Antibiotic resistance: Paromomycin
Sensitivity: short-term light fluctuations
Reference strain: CC-5155 and CC-4534
Burlacot A, Richaud P, Gosset A, Li-Beisson Y, Peltier G. Algal photosynthesis converts nitric oxide into nitrous oxide. Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2704-2709. doi: 10.1073/pnas.1915276117. Epub 2020 Jan 15. PMID: 31941711; PMCID: PMC7007583.
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