Strains

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf23::aphVII; ac17; atg17::aphIII

Strain CC-5909 was transformed with aphVII construct targeted to exon 1 of the PF23 gene using CRISPR/Cas9.

This strain is a null allele of PF23. It lacks cilia under vegetative conditions and has extremetly short, stubby cilia as gametes. It does not grow in medium lacking acetate and is resistant to hygromycin and paromomycin.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Payne ZL, Penny GM, Turner TN, Dutcher SK. A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis. Plant Commun. 2023 Mar 13;4(2):100493. doi: 10.1016/j.xplc.2022.100493. Epub 2022 Nov 17. PMID: 36397679; PMCID: PMC10030371.

Lin H, Cliften PF, Dutcher SK. MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 2018 Dec;178(4):1436-1447. doi: 10.1104/pp.18.00474. Epub 2018 Sep 11. PMID: 30206105; PMCID: PMC6288735.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf23::aphVII; ac17; atg17::aphIII

Strain CC-5908 was transformed with aphVII construct targeted to exon 1 of the PF23 gene using CRISPR/Cas9.

This strain is a null allele of PF23. It lacks cilia under vegetative conditions and has extremetly short, stubby cilia as gametes. It does not grow in medium lacking acetate and is resistant to hygromycin and paromomycin.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Payne ZL, Penny GM, Turner TN, Dutcher SK. A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis. Plant Commun. 2023 Mar 13;4(2):100493. doi: 10.1016/j.xplc.2022.100493. Epub 2022 Nov 17. PMID: 36397679; PMCID: PMC10030371.

Lin H, Cliften PF, Dutcher SK. MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 2018 Dec;178(4):1436-1447. doi: 10.1104/pp.18.00474. Epub 2018 Sep 11. PMID: 30206105; PMCID: PMC6288735.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

New wild-type strain generated from multiple crosses of CC-124 and CC-125. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This mutant is a progeny from a cross between pf23-4 and cnk11-3. Motility resembles wild-type.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Lin H, Zhang Z, Guo S, Chen F, Kessler JM, Wang YM, Dutcher SK. A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures. PLoS Genet. 2015 Sep 8;11(9):e1005508. doi: 10.1371/journal.pgen.1005508. PMID: 26348919; PMCID: PMC4562644.

  • Locus:
  • CNK11, PF23
  • Chromosome:
  • 7, 11

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This mutant is a progeny from a cross between pf23-4 and cnk11-3. Motility resembles wild-type.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Lin H, Zhang Z, Guo S, Chen F, Kessler JM, Wang YM, Dutcher SK. A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures. PLoS Genet. 2015 Sep 8;11(9):e1005508. doi: 10.1371/journal.pgen.1005508. PMID: 26348919; PMCID: PMC4562644.

  • Locus:
  • CNK11, PF23
  • Chromosome:
  • 7, 11

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This mutant is acilliate or has short, stubby cilia and is resistant to hygromycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Lin H, Zhang Z, Guo S, Chen F, Kessler JM, Wang YM, Dutcher SK. A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures. PLoS Genet. 2015 Sep 8;11(9):e1005508. doi: 10.1371/journal.pgen.1005508. PMID: 26348919; PMCID: PMC4562644.

  • Locus:
  • PF23, CNK11
  • Chromosome:
  • 11, 7

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This mutant is acilliate or has short, stubby cilia and is resistant to hygromycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Lin H, Zhang Z, Guo S, Chen F, Kessler JM, Wang YM, Dutcher SK. A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures. PLoS Genet. 2015 Sep 8;11(9):e1005508. doi: 10.1371/journal.pgen.1005508. PMID: 26348919; PMCID: PMC4562644.

  • Locus:
  • PF23, CNK11
  • Chromosome:
  • 11, 7

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This is a rescued strain and motility resembles wild-type. It was generated from transformation of strain pf13-1 with wild-type PF13 gene tagged at the C-terminus with YFP-FLAG tag generated through recombineering.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF13
  • Chromosome:
  • 9

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This strain was generated from transformation of strain pf23-1 with a wild-type PF23 gene tagged at the C-terminus with a m-scarlet-FLAG tag generated through recombineering.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23
  • Chromosome:
  • 11

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

This strain was generated from transformation of strain pf23-1 with a wild-type PF23 gene tagged at the C-terminus with a neon green-FLAG tag generated through recombineering.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23
  • Chromosome:
  • 11

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Strain is aciliate/has short cilia. It was generated from strain CC-5908 transformed with aphVII and backcrossed twice to CC-124.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Strain is aciliate/has short cilia. It was generated from strain CC-5908 transformed with aphVII and backcrossed twice to CC-124. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Strain is aciliate/has short cilia. It was generated from strain CC-5908 transformed with aphVII and backcrossed twice to CC-124. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Strain is aciliate/has short cilia. It was generated from strain CC-5908 transformed with aphVII and backcrossed twice to CC-124. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

  • Locus:
  • PF23, AC17, ATG17
  • Chromosome:
  • 11, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda1; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

This is a cross of oda1 CC-2228 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Takada S, Wilkerson CG, Wakabayashi K, Kamiya R, Witman GB. The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly. Mol Biol Cell. 2002 Mar;13(3):1015-29. doi: 10.1091/mbc.01-04-0201. PMID: 11907279; PMCID: PMC99616.

  • Locus:
  • ODA1 [DCC2], AC17, ATG17
  • Chromosome:
  • 16, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda2-921; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

This is a cross of the oda2 CLiP strain LMJ.RY0402.220921 with CC-5908. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, Jonikas MC. A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18. PMID: 30886426; PMCID: PMC6636631.

  • Locus:
  • ODA2, AC17, ATG17
  • Chromosome:
  • 11, 3,16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda2-921; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

This is a cross of the oda2 CLiP strain LMJ.RY0402.220921 with CC-5908. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, Jonikas MC. A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18. PMID: 30886426; PMCID: PMC6636631.

  • Locus:
  • ODA2, AC17, ATG17
  • Chromosome:
  • 11, 3,16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda3-1; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

This is a cross of oda3 CC-2232 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Koutoulis A, Pazour GJ, Wilkerson CG, Inaba K, Sheng H, Takada S, Witman GB. The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex. J Cell Biol. 1997 Jun 2;137(5):1069-80. doi: 10.1083/jcb.137.5.1069. Erratum in: J Cell Biol 1997 Aug 11;138(3):729. PMID: 9166407; PMCID: PMC2136212.

  • Locus:
  • ODA3, AC17, ATG17
  • Chromosome:
  • 17, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda4; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

This is a cross of oda4 CC-2234 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Kamiya R. Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii. J Cell Biol. 1988 Dec;107(6 Pt 1):2253-8. doi: 10.1083/jcb.107.6.2253. PMID: 2974040; PMCID: PMC2115671.

  • Locus:
  • ODA4, AC17, ATG17
  • Chromosome:
  • 9, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: sup-pf1-1; ac17; ATG17:aphVIII-TG
Phenotype: Impaired motility

pf6; sup-pf1 CC-3906 was crossed with CC-125. Progeny with sup-pf1 genotype was subsequently crossed to CC-5908.  It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Kamiya R. Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii. J Cell Biol. 1988 Dec;107(6 Pt 1):2253-8. doi: 10.1083/jcb.107.6.2253. PMID: 2974040; PMCID: PMC2115671.

  • Locus:
  • ODA4, AC17, ATG17
  • Chromosome:
  • 10, 3,16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda5; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda5 CC-2236 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB. Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell. 2004 Jun;15(6):2729-41. doi: 10.1091/mbc.e03-11-0820. Epub 2004 Apr 2. PMID: 15064350; PMCID: PMC420097.

  • Locus:
  • ODA5, AC17, ATG17
  • Chromosome:
  • 7, 3,16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda6; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda6 CC-2238 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Mitchell DR, Kang Y. Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene. J Cell Biol. 1991 May;113(4):835-42. doi: 10.1083/jcb.113.4.835. PMID: 1673970; PMCID: PMC2288990.

Mitchell DR, Kang Y. Reversion analysis of dynein intermediate chain function. J Cell Sci. 1993 Aug;105 ( Pt 4):1069-78. doi: 10.1242/jcs.105.4.1069. PMID: 8227195.

  • Locus:
  • ODA6 [DIC2], AC17, ATG17
  • Chromosome:
  • 12, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda7; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda7 CC-2240 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Duquesnoy P, Escudier E, Vincensini L, Freshour J, Bridoux AM, Coste A, Deschildre A, de Blic J, Legendre M, Montantin G, Tenreiro H, Vojtek AM, Loussert C, Clément A, Escalier D, Bastin P, Mitchell DR, Amselem S. Loss-of-function mutations in the human ortholog of Chlamydomonas reinhardtii ODA7 disrupt dynein arm assembly and cause primary ciliary dyskinesia. Am J Hum Genet. 2009 Dec;85(6):890-6. doi: 10.1016/j.ajhg.2009.11.008. PMID: 19944405; PMCID: PMC2790569.

  • Locus:
  • ODA7, AC17, ATG17
  • Chromosome:
  • 1, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda8; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda8 CC-2242 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.)

Desai PB, Freshour JR, Mitchell DR. Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes. Cytoskeleton (Hoboken). 2015 Jan;72(1):16-28. doi: 10.1002/cm.21206. Epub 2015 Feb 7. PMID: 25558044; PMCID: PMC4361367.

  • Locus:
  • ODA8, AC17, ATG17
  • Chromosome:
  • 1, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda9; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda9 CC-2244 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Wilkerson CG, King SM, Koutoulis A, Pazour GJ, Witman GB. The 78,000 M(r) intermediate chain of Chlamydomonas outer arm dynein isa WD-repeat protein required for arm assembly. J Cell Biol. 1995 Apr;129(1):169-78. doi: 10.1083/jcb.129.1.169. PMID: 7698982; PMCID: PMC2120364.

Fowkes ME, Mitchell DR. The role of preassembled cytoplasmic complexes in assembly of flagellar dynein subunits. Mol Biol Cell. 1998 Sep;9(9):2337-47. doi: 10.1091/mbc.9.9.2337. PMID: 9725897; PMCID: PMC25499.

  • Locus:
  • ODA9, AC17, ATG17
  • Chromosome:
  • 12, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda10; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda10 CC-2247 with CC-5909. It requires acetate for growth and is resistant to paramomycin. 

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Dean AB, Mitchell DR. Chlamydomonas ODA10 is a conserved axonemal protein that plays a unique role in outer dynein arm assembly. Mol Biol Cell. 2013 Dec;24(23):3689-96. doi: 10.1091/mbc.E13-06-0310. Epub 2013 Oct 2. PMID: 24088566; PMCID: PMC3842995.

Dean AB, Mitchell DR. Late steps in cytoplasmic maturation of assembly-competent axonemal outer arm dynein in Chlamydomonas require interaction of ODA5 and ODA10 in a complex. Mol Biol Cell. 2015 Oct 15;26(20):3596-605. doi: 10.1091/mbc.E15-05-0317. Epub 2015 Aug 26. PMID: 26310446; PMCID: PMC4603930.

  • Locus:
  • ODA10, AC17, ATG17
  • Chromosome:
  • 8, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda11; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda11 CC-2672 with CC-5909. It requires acetate for growth and is resistant to paramomycin. This strain has a second hit in the background that causes it to have short cilia and not swim properly.

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Sakakibara H, Mitchell DR, Kamiya R. A Chlamydomonas outer arm dynein mutant missing the α heavy chain. Journal of Cell Biology. 1991;113: 615–622. doi:10.1083/jcb.113.3.615

  • Locus:
  • ODA11, AC17, ATG17
  • Chromosome:
  • 7, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda12; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda12-2 CC-3941 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Pazour GJ, Koutoulis A, Benashski SE, Dickert BL, Sheng H, Patel-King RS, King SM, Witman GB. LC2, the chlamydomonas homologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly. Mol Biol Cell. 1999 Oct;10(10):3507-20. doi: 10.1091/mbc.10.10.3507. PMID: 10512883; PMCID: PMC25620.

  • Locus:
  • ODA12, AC17, ATG17
  • Chromosome:
  • 12, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda14; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda14 (F28) CC-3998 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Casey DM, Yagi T, Kamiya R, Witman GB. DC3, the Smallest Subunit of the Chlamydomonas Flagellar Outer Dynein Arm-docking Complex, Is a Redox-sensitive Calcium-binding Protein. Journal of Biological Chemistry. 2003;278: 42652–42659. doi:10.1074/jbc.M303064200

  • Locus:
  • ODA14, AC17, ATG17
  • Chromosome:
  • 1, 3, 16

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: oda16; ac17; ATG17:aphVIII-TG
Phenotype: Impaired mobility

This is a cross of oda16 CC-4554 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Ahmed NT, Mitchell DR. ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell. 2005 Oct;16(10):5004-12. doi: 10.1091/mbc.e05-07-0627. Epub 2005 Aug 10. PMID: 16093345; PMCID: PMC1237099.

  • Locus:
  • ODA16, AC17, ATG17
  • Chromosome:
  • 4, 3, 16