Strains Archives - Page 128 of 130 - Chlamydomonas Resource Center

* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

CC-6103 pf13-1; ac17; aphVIII mt+

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf13-1; ac17; ATG17:aphVIII-TG
Phenotype: Aciliate/short cilia

This is a cross of pf13 CC-1030 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Penny GM, Dutcher SK. Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii. PLoS Genet. 2024 Mar 18;20(3):e1011038. doi: 10.1371/journal.pgen.1011038. PMID: 38498551; PMCID: PMC11020789.

Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H. Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature. 2008 Dec 4;456(7222):611-6. doi: 10.1038/nature07471. PMID: 19052621; PMCID: PMC3279746.

Locus:
PF13, AC17, ATG17
Chromosome:
9, 3, 16

CC-6104 pf14; ac17; aphVIII mt+

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf14; ac17; ATG17:aphVIII-TG
Phenotype: Paralyzed flagella

This is a cross of pf14 CC-1032 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Williams BD, Velleca MA, Curry AM, Rosenbaum JL. Molecular cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: flagellar mutation pf-14 is an ochre allele. J Cell Biol. 1989 Jul;109(1):235-45. doi: 10.1083/jcb.109.1.235. PMID: 2745550; PMCID: PMC2115482.

Locus:
PF14, AC17, ATG17
Chromosome:
6, 3, 16

CC-6105 pf22; ac17; aphVIII mt-

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf22; ac17; ATG17:aphVIII-TG
Phenotype: Aciliate/short cilia

This is a cross of pf22 CC-1382 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Schmidts M, Freshour J, Loges NT, Dritsoula A, Antony D, Hirst RA, et al. Mutations in the dynein assembly factor PF22 (DNAAF3) cause primary ciliary dyskinesia with absent dynein arms. Cilia 2012 1:1. 2012;1: 1–1. doi:10.1186/2046-2530-1-S1-P101

Locus:
PF22, AC17, ATG17
Chromosome:
1, 3, 16

CC-6106 pf23-1 mt+

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: pf23-1; ac17; ATG17:aphVIII-TG
Phenotype: Aciliate/short cilia

This is a cross of pf23-1 CC-3660 with CC-5909. It requires acetate for growth and is resistant to paramomycin

Yamamoto R, Obbineni JM, Alford LM, Ide T, Owa M, Hwang J, Kon T, Inaba K, James N, King SM, Ishikawa T, Sale WS, Dutcher SK. Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms. PLoS Genet. 2017 Sep 11;13(9):e1006996. doi: 10.1371/journal.pgen.1006996. Erratum in: PLoS Genet. 2017 Oct 23;13(10 ):e1007063. PMID: 28892495; PMCID: PMC5608425.

Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, Köhler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K; UK10K; Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran H. DYX1C1 is required for axonemal dynein assembly and ciliary motility. Nat Genet. 2013 Sep;45(9):995-1003. doi: 10.1038/ng.2707. Epub 2013 Jul 21. PMID: 23872636; PMCID: PMC4000444.

Locus:
PF23, AC17, ATG17
Chromosome:
11, 3, 16

CC-6107 wdr92 mt+

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: wdr92-495; ac17; ATG17:aphVIII-TG
Phenotype: Aciliate/short cilia

This is a cross of wdr92-1; tpg1-2 CC-5520 with wild-type and then with CC-5909. It requires acetate for growth and is resistant to paramomycin

Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, Jonikas MC. A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18. PMID: 30886426; PMCID: PMC6636631.

Patel-King RS, Sakato-Antoku M, Yankova M, King SM. WDR92 is required for axonemal dynein heavy chain stability in cytoplasm. Mol Biol Cell. 2019 Jul 15;30(15):1834-1845. doi: 10.1091/mbc.E19-03-0139. Epub 2019 May 22. PMID: 31116681; PMCID: PMC6727741.

Locus:
WRD92, AC17, ATG17
Chromosome:
16, 3, 16

CC-6108 wdr92 mt-

$30.00

From Gervette Penny, Susan Dutcher lab, Washington Univerity in St. Loius, November 2023

Genotype: wdr92-495; ac17; ATG17:aphVIII-TG
Phenotype: Aciliate/short cilia

This is a cross of wdr92-1; tpg1-2 CC-5520 with wild-type and then with CC-5909. It requires acetate for growth and is resistant to paramomycin

Locus:
WRD92, AC17, ATG17
Chromosome:
16, 3, 16

CC-6109 cehc1-1; crr1-1::ble; mt- [CS0117C4]

$30.00

From Carolyn Silflow, University of Minnesota, February 2024

This strain contains a mutation in gene Cre01.g005900, identified by the cehc1-1 mutation. It encodes an FBOX3 protein that acts upstream of transcription factor CRR1. The strain also contains the crr1-1 mutation.

Sun X, LaVoie M, Lefebvre PA, Gallaher SD, Glaesener AG, Strenkert D, Mehta R, Merchant SS, Silflow CD. Mutation of negative regulatory gene CEHC1 encoding an FBXO3 protein results in normoxic expression of HYDA genes in Chlamydomonas reinhardtii. bioRxiv [Preprint]. 2024 Mar 27:2024.03.22.586359. doi: 10.1101/2024.03.22.586359. PMID: 38586028; PMCID: PMC10996464.

Locus:
CEHC1
Chromosome:
1

CC-6110 cehc1-1; crr1-1::ble; mt+ [CS0117F4]

$30.00

From Carolyn Silflow, University of Minnesota, February 2024

Locus:
CEHC1
Chromosome:
1

CC-6111 trp11

$30.00

From Kenjiro Yoshimura, Shibaura Institute of Technology-Japan, March 2024

Origin: CLiP mutant (LMJ.RY0402.150923), backcrossed to wild type three times
Comment: Defective in mechanoresponses

Oshima D, Yoshida M, Saga K, Ito N, Tsuji M, Isu A, Watanabe N, Wakabayashi KI, Yoshimura K. Mechanoresponses mediated by the TRP11 channel in cilia of Chlamydomonas reinhardtii. iScience. 2023 Sep 15;26(10):107926. doi: 10.1016/j.isci.2023.107926. PMID: 37790279; PMCID: PMC10543168.

CC-6112 ppr2

$30.00

From Kenjiro Yoshimura, Shibaura Institute of Technology-Japan, March 2024

Origin: nit1cw15, backcrossed to wild type 137c twice
Comment: Defective in photoshock and mechanoshock responses because of a defect in voltage-dependent calcium channel, CAV2

Matsuda A, Yoshimura K, Sineshchekov OA, Hirono M, Kamiya R. Isolation and characterization of novel Chlamydomonas mutants that display phototaxis but not photophobic response. Cell Motil Cytoskeleton. 1998;41(4):353-62. doi: 10.1002/(SICI)1097-0169(1998)41:4<353::AID-CM7>3.0.CO;2-0. PMID: 9858159.

Fujiu K, Nakayama Y, Yanagisawa A, Sokabe M, Yoshimura K. Chlamydomonas CAV2 encodes a voltage- dependent calcium channel required for the flagellar waveform conversion. Curr Biol. 2009 Jan 27;19(2):133-9. doi: 10.1016/j.cub.2008.11.068. PMID: 19167228.

CC-6113 trp2-3

$30.00

From Kenjiro Yoshimura, Shibaura Institute of Technology-Japan, March 2024

Origin: CLiP mutant (LMJ. RY0402.055255), backcrossed to wild type three times
Comment: Defective in thermotaxis and temperature-dependent augmentation of ciliary motility

Fueki S, Kaneko T, Matsuki H, Hashimoto Y, Yoshida M, Isu A, Wakabayashi KI, Yoshimura K. Temperature-dependent augmentation of ciliary motility by the TRP2 channel in Chlamydomonas reinhardtii. Cytoskeleton (Hoboken). 2024 Mar 1. doi: 10.1002/cm.21840. Epub ahead of print. PMID: 38426808.