Strains
CC-6140 tpt2-ko1
$30.00
$30.00
From Justin Findinier, Grossman lab, Carnegie Institution, Stanford CA, April 2024
Locus: Cre06.g263850
Background: M10
Origin: in lab, CRISPR knock out of TPT2 gene with hygromycin cassette insertion
Culture maintenance: low light (30-50uE) with acetate
Huang W, Krishnan A, Plett A, Meagher M, Linka N, Wang Y, Ren B, Findinier J, Redekop P, Fakhimi N, Kim RG, Karns DA, Boyle N, Posewitz MC, Grossman AR. Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive. Plant Cell. 2023 Jun 26;35(7):2592-2614. doi: 10.1093/plcell/koad095. PMID: 36970811; PMCID: PMC10291034.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.234057 (with mutation in cpld64 genes) was rescued by pRAM118 based plasmid with Cre12.g485850-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.228123 (with mutation in chlm genes) was rescued by pRAM118 based plasmid with Cre12.g498550-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.044496 (with mutation in pir1 genes) was rescued by pRAM118 based plasmid with Cre01.g014000-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.057931 (with mutation in cgl54 genes) was rescued by pRAM118 based plasmid with Cre02.g073850-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.248779 (with mutation in pmr1 genes) was rescued by pRAM118 based plasmid with Cre10.g448950-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.046095 (with mutation in cpl6 genes) was rescued by pRAM118 based plasmid with Cre06.g279500-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.208103 (with mutation in raa6 genes) was rescued by pLM005 based plasmid with Cre07.g350700-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.077016 (with mutation in psr1 genes) was rescued by pRAM118 based plasmid with Cre10.g433400-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.133008 (with mutation in raa17 genes) was rescued by pLM005 based plasmid with Cre13.g566400-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.164167 (with mutation in tba2 genes) was rescued by pRAM118 based plasmid with Cre13.g578650-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.207089 (with mutation in tpk1 genes) was rescued by pRAM118 based plasmid with Cre01.g040050-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.254076 (with mutation in raa15 genes) was rescued by pLM005 based plasmid with Cre17.g728850-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.193706 (with mutation in mtf1 genes) was rescued by pRAM118 based plasmid with Cre12.g560550-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.176891 (with mutation in psr5 genes) was rescued by pRAM118 based plasmid with Cre01.g022681-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin and hygromycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From Moshe Kafri, Jonikas lab, Princeton University, May 2024
The CLiP strain LMJ.RY0402.255772 (with mutation in pbs27 genes) was rescued by pLM005 based plasmid with Cre05.g243800-Venus-3xFLAG. This strain includes abiotic resistance for paromomycin.
Kafri M, Patena W, Martin L, Wang L, Gomer G, Ergun SL, Sirkejyan AK, Goh A, Wilson AT, Gavrilenko SE, Breker M, Roichman A, McWhite CD, Rabinowitz JD, Cross FR, Wühr M, Jonikas MC. Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery. Cell. 2023 Dec 7;186(25):5638-5655.e25. doi: 10.1016/j.cell.2023.11.007. PMID: 38065083; PMCID: PMC10760936.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024.
This is an aCRY disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: pCRY, Cre06.g295200
Target sequence: GCGACATGCTGTATGAGCCG (Exon2)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
CC-6176 ∆aCry-E8 [PH244]
$30.00
$30.00
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an aCRY disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: aCRY, Cre06.g278251
Target sequence: GCTGTGTTTTGAGCACGACA (Exon 5)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a pCRY (CPH1) disruption strain, generated with CRISPR/Cas9. The pCRY strain contains an insertion of the marker plasmid pAphVIII (pPH75).
Background strain: SAG 73-72 (from Takuya Matsuo, see Niwa et al. 2013)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: pCRY (CPH1), Cre06.g295200
Target sequence: GCGACATGCTGTATGAGCCG (Exon2)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a pCRY (CPH1) disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 73-72 (from Takuya Matsuo, see Niwa et al. 2013)
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: pCRY (CPH1), Cre06.g295200
Target sequence: GCGACATGCTGTATGAGCCG (Exon2)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a strain with a mutated C128 residue to T128 in ChR2 ( = point mutation C128T) and disruption of ChR1, which was generated with SpCas9 in the CC-125 background.
Background strain: CC-125 mt+
Nuclease: SpCas9
Target gene: ChR1, Cre14.g611300; ChR2, Cre02.g085257
Target sequence: TGTGGCTTCGTTACGCGGAG (ChR1); AGTGGTTGCGTTACGCCGAG (ChR2)
Marker: pAPHVIII (pPH75); pAphVII (pPH360)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an AGG3 disruption strain, generated with SpCas9 in the SAG 11-32b background.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: SpCas9
Target gene: AGG3, Cre10.g456100
Target sequence: GAAGCCGAACACGAAGCCAT
Marker: pAPHVIII (pPH75)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a pCry disruption strain, generated with SpCas9 in the CC-124 background.
Background strain: CC-124
Nuclease: SpCas9
Target gene: pCry, Cre06.g295200
Target sequence: TTAGGGTCCAGCACATCCCA
Marker: pAPHVIII (pPH75)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a pCry disruption strain, generated with SpCas9 in the CC-124 background.
Background strain: CC-124
Nuclease: SpCas9
Target gene: pCry, Cre06.g295200
Target sequence: TTAGGGTCCAGCACATCCCA
Marker: pAPHVIII (pPH75)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an aCRY pCRY disruption strain, generated with CRISPR/Cas9.
Background strain: PH254, SAG 11-32b [=CC-409 mt+ = UTEX 90]
Marker: pAphVII (pPH360); pAPHVIII (pPH75)
Target gene: pCRY (CPH1), Cre06.g295200; aCRY, Cre06.g278251
Target sequence: pCRY – GACCTAGAGTGTGATGCGCT (Exon6); aCRY – GCTGTGTTTTGAGCACGACA (Exon 5)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Darius Rauch, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an aCRY disruption strain, generated with CRISPR/Cas9.
Background strain: SAG 11-32b [=CC-409 mt+ = UTEX 90]
Nuclease: (Sp)Cas9 as ribonucleoprotein (RNP)
Marker: pAPHVIII (pPH75)
Target gene: aCRY, Cre06.g278251
Target sequence: GCTGTGTTTTGAGCACGACA (Exon 5)
This is an unpublished strain. Please contact ph@chlamy.de before using it.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an ARL11 disruption strain, generated with SpCas9 in the CC-3403 background.
Background strain: CC-3403
Nuclease: SpCas9
Target gene: ARL11 Cre12.g487850
Target sequence: ATTACTTCAACAACACGGAT
Marker: pHR11
This strain was published in Wolfram et al doi.org/10.22541/au.171488985.58752434/v1. If you use the strain, please cite it accordingly.
Wolfram M, Greif A, Baidukova O, Voll H, Tauber S, Lindacher J, Hegemann P, Kreimer G. Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Plant Cell Environ. 2024 Jun 27. doi: 10.1111/pce.15017. Epub ahead of print. PMID: 38935876.
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is an ARL11 disruption strain, generated with SpCas9 in the CC-3403 background.
Background strain:CC-3403
Nuclease:SpCas9
Target gene: ARL11 Cre12.g487850
Target sequence: ATCCTCTACAAGCTTCACAT
Marker: pHR11
This strain was published in Wolfram et al doi.org/10.22541/au.171488985.58752434/v1. If you use the strain, please cite it accordingly.
Wolfram M, Greif A, Baidukova O, Voll H, Tauber S, Lindacher J, Hegemann P, Kreimer G. Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Plant Cell Environ. 2024 Jun 27. doi: 10.1111/pce.15017. Epub ahead of print. PMID: 38935876.
CC-6187 ∆CEP-1 [PH266]
$30.00
$30.00
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a CEP1 disruption strain, generated with SpCas9 in the CC-3403 background.
Background strain: CC-3403
Nuclease: SpCas9
Target gene: CEP1 Cre09.g407700
Target sequence: AAGATGGCTCTTAGATTGCT
Marker: pHR11
This strain was published in Wolfram et al doi.org/10.22541/au.171488985.58752434/v1doi.org/10.22541/au.171488985.58752434/v1. If you use the strain, please cite it accordingly.
Wolfram M, Greif A, Baidukova O, Voll H, Tauber S, Lindacher J, Hegemann P, Kreimer G. Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Plant Cell Environ. 2024 Jun 27. doi: 10.1111/pce.15017. Epub ahead of print. PMID: 38935876.
CC-6188 ∆CEP-3 [PH268]
$30.00
$30.00
Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University of Berlin, May 2024
This is a CEP disruption strain, generated with SpCas9 in the CC3403 background.
Background strain: CC-3403
Nuclease: SpCas9
Target gene: CEP1 Cre09.g407700
Target sequence: TTCTTGCGCCAATCGATGGC
Marker: pHR11
This strain was published in Wolfram et al doi.org/10.22541/au.171488985.58752434/v1. If you use the strain, please cite it accordingly.
Wolfram M, Greif A, Baidukova O, Voll H, Tauber S, Lindacher J, Hegemann P, Kreimer G. Insights into degradation and targeting of the photoreceptor channelrhodopsin-1. Plant Cell Environ. 2024 Jun 27. doi: 10.1111/pce.15017. Epub ahead of print. PMID: 38935876.