Strains
CC-1004 ac15 NIT+ mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University, May 1980
Phenotype: requires acetate
From a cross of CC-527 ac15 x CC-919 wild type 11/32b (NIT+) [no longer in the collection]
This strain provides the ac15 marker on linkage group IX with the wild type alleles of the linked marker NIT1 and the unlinked NIT2. It can grow on nitrate as its sole N source.
CC-1006 ac51 pf13 mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University, May 1980
Phenotype: requires acetate; motility impaired
From a cross of CC-611 pf13 mt+ x CC-7 ac51 mt-
This strain couples distal markers on both arms of linkage group IX. The ac51 marker is somewhat difficult to score; the phenotype is clearer at high pH (make Tris-minimal medium and adjust the pH to 8.3).
From the University of Texas Algal Collection (UTEX), May 1980
This is a wild-type strain tracing back to the 1945 collection by G.M. Smith, near Amherst MA, but it has been separate from the 137c (CC-124 and CC-125) and Sager (CC-1690) lines since about 1950. This strain can grow on nitrate.
For more information on the origin of the standard laboratory strains of C. reinhardtii, please see the following paper:
Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610
From the University of Texas Algal Collection (UTEX), May 1980
This is a wild-type strain tracing back to the 1945 collection by G.M. Smith, near Amherst MA, but it has been separate from the 137c (CC-124 and CC-125) and Sager (CC-1690) lines since about 1950. This strain can grow on nitrate.
For more information on the origin of the standard laboratory strains of C. reinhardtii, please see the following paper:
Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610
Boynton-Gillham laboratory, Duke University
Phenotype: requires acetate
This mutation consists of deletion of one entire rRNA region plus the adjoining unique sequence region of Eco 11/Bam 10, producing an atpB-deficient phenotype. The chloroplast ribosomes appear normal.
A fusion fragment was sequenced by Scott Newman in 1989, unpublished. The mutation appears to have arisen by illegitimate recombination between dispersed short repeat segments flanking the atpB and 5S rRNA genes.
Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357
Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151
Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190
CC-1017 ac1a mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University
Phenotype: requires acetate
From CC-514 ac1a mt+ x CC-262 pf17 mt-, a product from a PD tetrad that carries ac1a in mt- but does not carry the pf17 mutation. A cross to CC-770 spr1 gave some clean acetate-requiring progeny and others that grew somewhat on miminal medium, suggestive of a segregating suppressor.
See CC-514 for additional information on ac1a.
CC-1019 ac22 mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University
Phenotype: requires acetate
Derived from a series of two backcrosses of CC-534 ac22 (suppressed) to wild type.
This mutation was UV-induced in Levine’s laboratory.
Levine RP, Goodenough UW (1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408
CC-1020 ac12 pf12 mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University
Phenotype: requires acetate; motility impaired
From a cross of CC-609 pf12 [stock no longer extant] x CC-523 ac12a mt-
This strain was created to couple the markers ac12 and pf12 on linkage group II.
In a subsequent cross of CC-1020 to a NIT strain (CC-270), the nitrate reductase deficientphenotype segregated 2:2, indicating that CC-1020 carries one nit marker only. This was surprising since CC-609 and CC-523 were both Levine stocks and therefore expected to carry nit1 nit2. The nit marker in this strain appears to be nit1.
From Robert Hodson, University of Delaware, April 1980
Strain 2137 was isolated by Robert Spreitzer in the laboratory of Laurie Mets, from a cross of Sager’s 21 gr strain (equivalent to CC-1690) to 137c mt- (equivalent to CC-124). A product was selected for ability to grow as single cells in minimal medium, for negative phototaxis (agg1) and for green color when grown in the dark. Subsequently many mutants have been isolated in this background.
Please see CC-3269 for another 2137 stock, obtained directly from Laurie Mets. Unless you have already used CC-1021 and want the identical strain for comparison, please order CC-3269.
This strain was originally reported to contain nit1 but not nit2 based on crosses done by the Chlamydomonas Genetics Center in the early 1980s. However, Olivier Vallon has found that it does not grow on nitrite medium, suggesting a nit2 mutation (pers. comm. August 1995). A cross to wild type nit+ carried out by the Chlamydomonas Genetics Center in September 1995 confirmed that this isolate does contain two nit mutations.
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569
CC-1024 pf1 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained his pf1 strain from Warr, which makes us suspect that this is one of the two pf1 alleles isolated by McVittie rather than the original pf1 isolated by Lewin and subsequently characterized in Levine’s laboratory (see CC-602). This strain is derivative pf1.31D+ from Luck’s lab.
Cells of the pf1 mutant lack flagellar spokeheads. Motility is impaired, and the phenotype is easy to score. The PF1 locus encodes flagellar radial spoke protein 4 (RSP4), a component of the radial spoke head.
McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM, Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB (2006) Radial spoke proteins of Chlamydomonas flagella. J Cell Sci 119(Pt 6):1165-1174
CC-1025 pf2 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Flagella in this mutant move erratically; suspensions typically contain some swimming cells.
The PF2 locus encodes component DRC4 of the dynein regulatory complex.
Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57
CC-1026 pf3 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
The original stock was obtained by Luck from Nam-Hai Chua, who got it from R.P. Levine. This stock is derivative pf3.1B+ from Luck’s lab.
Cells of this strain swim slowly.
This mutation appears to affect the dynein regulatory complex. Yanagisawa and Kamiya showed that both pf3 and ida6 are deficient in the tektin homolog p58, but that neither mutant mapped to the same position as this gene, nor did pf3 and ida6 appear to be allelic with one another.
It has been been reported by Susan Dutcher that this copy of CC-1026 pf3 mt+ carries a cnk11-6 mutation. Please see CC-5145 pf3 (wild-type for CNK11) for a pf3 strain with a verified wild type CNK11. -September 2015
Piperno G, Mead K, Shestak W (1992) The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella. J Cell Biol 118:1455-1463
Gardner LC, O'Toole E, Perrone CA, Giddings T, Porter ME (1994) Components of a "dynein regulatory complex" are located at the junction between the radial spokes and the dynein arms in Chlamydomonas flagella. J Cell Biol 127:1311-1325
Piperno G, Mead K, LeDizet M, Moscatelli A ( 1994) Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes. J Cell Biol 125:1109-1117
Yanagisawa HA, Kamiya R ( 2004) A tektin homologue is decreased in chlamydomonas mutants lacking an axonemal inner-arm dynein. Mol Biol Cell 15:2105-2115
CC-1027 pf4 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained the mutant from Chua who got it from Levine. This stock is Luck derivative pf3.1B+.
Cells of this strain swim slowly. The original mutant was described as difficult to mate. Elam et al. have shown that this is a mutation in the B subunit of the protein phosphatase PP2A.
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Elam CA, Wirschell M, Yamamoto R, Fox LA, York K, Kamiya R, Dutcher SK, Sale WS (2011) An axonemal PP2A B-subunit is required for PP2A localization and flagellar motility. Cytoskeleton 68:363-372
CC-1028 pf5 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s notes state that he obtained this mutant from Chua who got it from Levine. This stock is derivative pf5.6B+. An allelic mutant (pf5-A of Huang et al., 1981), was isolated in Luck’s laboratory. Huang et al. reported that their two pf5 mutants lacked four flagellar polypeptides: radial spoke components 13 and 15, component 1 of the innter dynein arms, and a low molecular weight axonemal protein whose structural localization was unknown.
Working with CC-1331, which was also originally designated pf5, Yang et al. (2009) determined that the mutation in that strain was in the FLA14 gene encoding the dynein light chain LC8, and renamed pf5 as fla14-3. They reported however that CC-1028 had a wild-type FLA14 gene, capable of rescuing the CC-1331 phenotype.
Since genetic analysis at the Chlamydomonas Genetics Center at Duke confirmed the expected linkage of the pf5 mutation in CC-1028 and its derivatives to linkage group III, we conclude that the mutation in CC-1028 and the one in CC-1331 are in different genes, but probably closely linked on the same chromosome.
Huang B, Piperno G, Ramanis Z, Luck DJ (1981) Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function. J Cell Biol 88:80-88
Yang P, Yang C, Wirschell M, Davis S (2009) Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes. J Biol Chem 284:31412-31421
CC-1029 pf6 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained this mutant from Chua who got it from Levine. This stock is derivative pf6.4D+.
Cells of this mutant ijiggle but cannot swim. Wargo et al. determined that it lacks the C1a projection on the central pair microtubules, and that it is also defective in calcium-induced modulation of dynein activity on specific doublet microtubules.
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
Rupp G, O'Toole E, Porter ME (2001) The Chlamydomonas PF6 locus encodes a large alanine/proline-rich polypeptide that is required for assembly of a central pair projection and regulates flagellar motility. Mol Biol Cell 12:739-751
Wargo MJ, Dymek EE, Smith EF (2005) Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus. J Cell Sci 118:4655-4665
Zhang Z, Jones BH, Tang W, Moss SB, Wei Z, Ho C, Pollack M, Horowitz E, Bennett J, Baker ME, Strauss JF 3rd (2005) Dissecting the axoneme interactome: the mammalian orthologue of Chlamydomonas PF6 interacts with sperm-associated antigen 6, the mammalian orthologue of Chlamydomonas PF16. Mol Cell Proteomics 4:914-923
Boesger J, Wagner V, Weisheit W, Mittag M (2009) Analysis of flagellar phosphoproteins from Chlamydomonas reinhardtii. Eukaryotic Cell 8:922-932
CC-1030 pf13 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
This is Luck’s strain pf13.4A+. We aren’t certain whether this is the original pf13 mutant isolated by Lewin, or the allelic mutant pf13a isolated by Huang et al. (please see CC-2492 for an unequivocal pf13a strain).
Mutants at this locus have a defect in the dynein outer arms. Flagella are short in exponentially growing cells but reach full length in stationary phase cultures. Motility is impaired, and it is not difficult to score.
Omran et al. showed that the PF13 locus corresponds to a gene encoding a conserved protein involved in assembly of outer arm dynein.
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Piperno G, Luck DJ (1981) Inner arm dyneins from flagella of Chlamydomonas reinhardtii. Cell 27:331-340
Witman GB, Minervini N (1982b) Role of calmodulin in the flagellar axoneme: effect of phenothiazines on reactivated axonemes of Chlamydomonas. Prog Clin Biol Res 80:199-204
Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskeleton 32:98-102
Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H (2008) Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. Nature 456:611-616
Yamamoto R, Hirono M, Kamiya R (2010) Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins. J Cell Biol 190:65-71
CC-1031 pf12 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
This is Luck’s strain pf12.1D+.
Flagella in pf12 are at an abnormal angle, and swimming is impaired. The cells show a slow-swimming, jiggling or twirling motion.
McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99
CC-1032 pf14 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained the pf14 mutant from Warr. This stock is Luck’s derivative pf14.5D+.
The pf14 mutation is in the gene encoding radial spoke protein 3. Motility is impaired, and the phenotype is easy to score. Please see CC-613 for more information on this mutant.
CC-1033 pf15 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck obtained this mutant from Levine. This stock is Luck’s derivative pf15.3G+.
The PF15 locus corresponds to the KAT2 gene encoding the katanin p80 subunit. Mutants at this locus lack the central pair of flagellar microtubules and are deficient in 18 axonemal polypeptides.
Dymek EE, Lefebvre PA, Smith EF (2004) PF15p is the chlamydomonas homologue of the Katanin p80 subunit and is required for assembly of flagellar central microtubules. Eukaryotic Cell 3:870-879
CC-1034 pf16 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s stock of this mutant was obtained from George Witman, and is presumed to be the original allele isolated by Lewin. This strain is Luck’s derivative pf16.28A+. Two allelic mutants were isolated in Luck’s laboratory, of which one is in our collection (CC-2510). Also see CC-3896 for a temperature-sensitive allele.
This mutation is in a gene encoding a flagellar central apparatus protein. The mutant phenotype is easy to score.
McVittie A (1972a) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
Bernstein M, Beech PL, Katz SG, Rosenbaum JL (1994) A new kinesin-like protein (Klp1) localized to a single microtubule of the Chlamydomonas flagellum. J Cell Biol 125:1313-1326
Frey E, Brokaw CJ, Omoto CK (1997) Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants. Cell Motil Cytoskeleton 38:91-99
Smith EF (2002b) Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase. Mol Biol Cell 13:3303-3313
CC-1035 pf17 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Cells with the pf17 mutation lack flagellar spokeheads and are missing five polypeptides of the radial spoke complex. Yang et al. showed that this is a mutation in the gene encoding radial spoke protein 9.
This mutation is a good marker for linkage group VII. Cells wiggle but don’t swim, and are easily scored in crosses.
Yang P, Diener DR, Yang C, Kohno T, Pazour GJ, Dienes JM, Agrin NS, King SM, Sale WS, Kamiya R, Rosenbaum JL, Witman GB (2006) Radial spoke proteins of Chlamydomonas flagella. J Cell Sci 119:1165-1174
CC-1036 pf18 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s stock was obtained from Levine. This strain is Luck’s derivative pf18.8D+.
The pf18 mutant has a central pair defect that causes the flagella to be rigid. Motility is completely impaired. These properties have made it an excellent system for investigation of movements along the flagellar membrane.
Warr JR, McVittie A, Randall JT, Hopkins JM (1966) Genetic control of flagellar structure in Chlamydomonas reinhardtii. Genet Res 7:335-351
Adams GM, Huang B, Piperno G, Luck DJ (1981) Central-pair microtubular complex of Chlamydomonas flagella: polypeptide composition as revealed by analysis of mutants. J Cell Biol 91:69-76
Bloodgood RA, May GS (1982) Functional modification of the Chlamydomonas flagellar surface. J Cell Biol 93:88-96
Bloodgood RA (1988) Gliding motility and the dynamics of flagellar membrane glycoproteins in Chlamydomonas reinhardtii. J Protozool 35:552-558
Goodenough UW (1993) Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii. Cell Motil Cytoskel 25:179-189
Wakabayashi K, Yagi T, Kamiya R (1997) Ca2+-dependent waveform conversion in the flagellar axoneme of Chlamydomonas mutants lacking the central-pair/radial spoke system. Cell Motil Cytoskeleton 38:22-28
Smith EF (2002a) Regulation of flagellar dynein by the axonemal central apparatus. Cell Motil Cytoskeleton 52:33-42
Smith EF (2002b) Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase. Mol Biol Cell 13:3303-3313
CC-1037 pf19 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s stock of this mutant was obtained from George Witman. This strain is Luck’s derivative pf19.24D+.
The pf19 mutant has a central pair defect. The flagella are paralyzed and rigid.
It has been been reported by Susan Dutcher that this copy of CC-1037 pf19 mt+ carries an imp3-2 mutation, which is different from the original imp3 mutation, but results in deletion in the same PP2A catalytic subunit. Please see CC-5150 pf19 (wild-type for IMP3) for a pf19 with a verified wild type IMP3. -September 2015
Warr JR, McVittie A, Randall JT, Hopkins JM (1966) Genetic control of flagellar structure in Chlamydomonas reinhardtii. Genet Res 7:335-351
Remillard SP and Witman GB (1982) Synthesis, transport, and utilization of specific flagellar proteins during flagellar regeneration in Chlamydomonas. J Cell Biol 93:615-31
Johnson KA, Haas MA, Rosenbaum JL (1994) Localization of a kinesin-related protein to the central pair apparatus of the Chlamydomonas reinhardtii flagellum. J Cell Sci 107:1551-1556
Hayashibe K, Shingyoji C, Kamiya R (1997) Induction of temporary beating in paralyzed flagella of Chlamydomonas mutants by application of external force. Cell Motil Cytoskeleton 37:232-239
CC-1038 pf20 mt+
$30.00
$30.00
From David Luck, Rockefeller University, 1980
Phenotype: impaired motility
Luck’s stock of this mutant was obtained from Chua who got it from Levine. This stock is Luck’s derivative pf20.3C+.
The PF20 locus encodes a WD-repeat containing protein of the central pair of the flagella, which associates with the intermicrotubule bridge. Homologs have been characterized in mammalian cells.
Warr JR, McVittie A, Randall JT, Hopkins JM (1966) Genetic control of flagellar structure in Chlamydomonas reinhardtii. Genet Res 7:335-351
Witman GB, Plummer J, Sander G (1978) Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components. J Cell Biol 76:729-747
Adams GM, Huang B, Piperno G, Luck DJ (1981) Central-pair microtubular complex of Chlamydomonas flagella: polypeptide composition as revealed by analysis of mutants. J Cell Biol 91:69-76
Smith EF and Lefebvre PA (1997) PF20 gene product contains WD repeats and localizes to the intermicrotubule bridges in Chlamydomonas flagella. Mol Biol Cell 8:455-467
CC-1042 F1 mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
This mutant lacks P700, CP1 and polypeptide 2 of the Photosystem I reaction center. Please see CC-978 for more information.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
CC-1043 F12 mt-
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
F12 is a mutation in the chloroplast PsaB RNA-binding protein encoded by the TAB2 locus. Another mutant, F14, is the only allele at this locus whose exact mutation is known, but F12, F20 and M27 were previously found to be in the same complementation group with F14.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
Dauvillée D, Stampacchia O, Girard-Bascou J, Rochaix JD (2003) Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA. EMBO J 22:6378-6388
CC-1044 F14 mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
F14 is a mutation (W348R) in the chloroplast PsaB RNA-binding protein encoded by the TAB2 locus. This is the only allele whose exact mutation is known, but three other strains, F14, F20 and M27, were previously found to be in the same complementation group with F14.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
Dauvillée D, Stampacchia O, Girard-Bascou J, Rochaix JD (2003) Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA. EMBO J 22, 6378-6388
CC-1045 F20 mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
F20 is a mutation in the chloroplast PsaB RNA-binding protein encoded by the TAB2 locus. Another mutant, F14, is the only allele at this locus whose exact mutation is known, but F12, F20 and M27 were previously found to be in the same complementation group with F14.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
Dauvillée D, Stampacchia O, Girard-Bascou J, Rochaix JD (2003) Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA. EMBO J 22:6378-6388
CC-1046 M27 mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
M27 is a mutation in the chloroplast PsaB RNA-binding protein encoded by the TAB2 locus. Another mutant, F14, is the only allele at this locus whose exact mutation is known, but F12, F20 and M27 were previously found to be in the same complementation group with F14.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
Dauvillée D, Stampacchia O, Girard-Bascou J, Rochaix JD (2003) Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA. EMBO J 22:6378-6388
CC-1047 F8 (ac211) mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, June 1980
Phenotype: requires acetate
The ac211 mutants are deficient in the PS I reaction center and were assigned to complementation group 3 by Girard et al. (1980), who showed that one of these mutants, M10, was linked to arg7 on linkage group I. Tang at al. showed that CC-1047 is deficient in photosystem I electron transport.
This linkage was confirmed by the Chlamydomonas Genetics Center, and the corresponding locus was named AC211, continuing Levine’s sequence of AC loci. It is about 4 centimorgans from ARG7, but has not been ordered relative to other loci in this region.
Girard J, Chua NH, Bennoun P, Schmidt G, Delosme M (1980) Studies on mutants deficient in the photosystem I reaction centers in Chlamydomonas reinhardtii. Curr Genet 2:215-221
Dauvillée D, Stampacchia O, Girard-Bascou J, Rochaix JD (2003) Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA. EMBO J 22:6378-6388
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