From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362