Strains Archives - Page 21 of 130 - Chlamydomonas Resource Center

* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

CC-1458 C. eugametos mpa-3 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 2

The mpa-3 mutation confers resistance to 3-methylphenylalanine. The resistance level is between that of mpa-3 and mpa1.

McBride AC, Gowans CS (1969) The induction of gene mutation and chromosome aberration in Chlamydomonas eugametos by a phenylalanine analog. Genet Res 14:121-126

CC-1459 C. eugametos sr mpa-2 female, possible translocation

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 4, annotated as “possible translocation.”

The mpa-2 mutation confers resistance to 3-methylphenylalanine, but at a lower level than mpa-1 or mpa-3. The sr mutation confers resistance to 50 micrograms/ml streptomycin.

Gowans CS (1960) Some genetic investigations on Chlamydomonas eugametos. Z Vererbungsl 91, 63-73

McBride JC, Gowans CS (1967) Pyrithiamine resistance in Chlamydomonas eugametos. Genetics 56:405-412

McBride AC, Gowans CS (1969) The induction of gene mutation and chromosome aberration in Chlamydomonas eugametos by a phenylalanine analog. Genet Res 14:121-126

CC-1460 C. eugametos nr-1 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain C 22-4

This mutant, which is resistant to neamine, was isolated in cells grown on 2-amino-3-phenylbutanoic acid (and is probably resistant to that compound also) prior to plating on neamine. It shows Mendelian inheritance (i.e. is in a gene encoded in nuclear DNA).

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344

CC-1461 C. eugametos ptr male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain Mo 71

This mutant is resistant to 4 micrograms/ml pyrithiamine.

McBride JC, Gowans CS (1967) Pyrithiamine resistance in Chlamydomonas eugametos. Genetics 56:405-412

CC-1464 C. eugametos sr-1

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 5

This is a nuclear streptomycin-resistant mutant recovered in cells grown on 2-amino-3-phenylbutanoic acid (APBA) prior to plating on streptomycin. It is resistant to 50 micrograms/ml but not 500 micrograms/ml streptomycin.

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344

CC-1465 C. eugametos sr-2 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 6

This is a chloroplast mutant resistant to 500 micrograms/ml streptomycin. The mutation was used extensively in studies by Lemieux and others on inheritance of chloroplast genes in crosses of C. eugametos x C. moewusii.

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J. Phycol 11:343-344

Lemieux C, Turmel M, Lee RW (1980) Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny. Curr Genet 2:139-147

Lemieux C, Turmel M, Seligy VL, Lee RW (1984) Chloroplast DNA recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonmendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc Natl Acad Sci U S A 81:1164-1168

CC-1466 C. eugametos spr female

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 8

This mutant is resistant to at least 20 micrograms/ml spectinomycin. See Gowans (1960) for methods used to isolate mutants in his laboratory; this particular mutant isn’t discussed, however.

Gowans CS (1960) Some genetic investigations on Chlamydomonas eugametos. Z Vererbungsl 91:63-73

CC-1467 C. eugametos nr male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 12

This is a nuclear neamine-resistant mutant recovered in cells grown on 2-amino-3-phenylbutanoic acid (APBA). It is resistant to 250 micrograms/ml streptomycin.

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344

CC-1468 C. eugametos tryp-sens female

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain Mo 74

This mutant, which was UV-induced by Nakamura and colleagues, grows poorly on tryptophan concentrations higher than 200 micrograms/ml.

Nakamura K, Kozloff RA, Gowans CS (1979) Isolation of tryptophan sensitive strains of the green alga Chlamydomonas. Env Exp Botany 19:311-314

CC-1469 C. eugametos sr male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 13

This is a spontaneous nuclear gene mutation conferring resistance to 50 micrograms/ml streptomycin.

Gowans CS (1960) Some genetic investigations on Chlamydomonas eugametos. Z Vererbungsl 91:63-73

CC-1470 C. eugametos mpa-1 sr-2 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain CJ 337-1

The mpa-1 mutation confers resistance to more than 500 micrograms/ml 3-methylphenylalanine (also called 2-amino-3-phenylbutanoic acid or APBA). sr-2 is a chloroplast mutation conferring resistance to 500 micrograms/ml streptomycin.

McBride JC, Gowans CS (1967) Pyrithiamine resistance in Chlamydomonas eugametos. Genetics 56:405-412

McBride AC, Gowans CS (1969) The induction of gene mutation and chromosome aberration in Chlamydomonas eugametos by a phenylalanine analog. Genet Res 14:121-126

McBride AC and McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J. Phycol 11:343-344

CC-1472 C. eugametos sr-2 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain CJ 338-2

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344

Lemieux C, Turmel M, Lee RW (1980) Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny. Curr Genet 2:139-147

CC-1473 C. eugametos sr-2 female

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain CJ 338-3

McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344

Lemieux C, Turmel M, Lee RW (1980) Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny. Curr Genet 2:139-147

CC-1474 C. eugametos n-8AG-2 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 8

This strain can use 200 micrograms/ml 8-azaguanine as its sole nitrogen source.

CC-1475 C. eugametos n-APBA-1 male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 9

This mutant can use 200 micrograms/ml 2-amino-3-phenylbutanoic acid (APBA) as its sole nitrogen source.

CC-1477 C. eugametos ery female

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain
J 262-2

This mutant is resistant to 50 grams/ml erythromycin. It shows Mendelian inheritance, indicating that it is in a nuclear gene.

CC-1478 C. eugametos ery male

$30.00

From C.S. Gowans, University of Missouri, March 1983, his strain H 11

This mutant is resistant to 50 grams/ml erythromycin. It shows Mendelian inheritance, indicating that it is in a nuclear gene.

CC-1480 C. moewusii L 1 mt+

$30.00

From C.S. Gowans, University of Missouri, March 1983

We believe that this is Lewin’s wild-type plus strain, equivalent to CC-55 [= UTEX 97]

CC-1481 C. moewusii L 2 mt-

$30.00

From C.S. Gowans, University of Missouri, March 1983

We believe that this is Lewin’s wild-type minus strain, equivalent to CC-56 [= UTEX 96].

CC-1482 C. moewusii M.475

$30.00

From C.S. Gowans, University of Missouri, March 1983, as L 7 475 n.m.

This motility mutant was isolated and described by Lewin. Both mating types can mate with other strains, but + and – strains both carrying this mutation do not mate well with each other. Flagella are slow to regenearte after deflagellation. This mutant is also in the collection as CC-957 and CC-2522.

Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363