Strains

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background. This mutants can complete more than one cell cycle at the restrictive temperature, but eventually blocks late in the cycle.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background. This mutant is blocked relatively early in the cell cycle.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

From Stephen Howell, UC San Diego, June 1983

Phenotype: temperature sensitive, cannot grow at 33 degrees

This is a mutant with a temperature-conditional cell cycle block, isolated in the 137c background.

Howell SH, Naliboff JA (1983) Conditional mutants in Chlamydomonas reinhardtii blocked in the vegetative cell cycle. I. An analysis of cell cycle block points. J Cell Biol 57:760-772

Elizabeth Harris, Boynton-Gillham laboratory, Duke University, 1983

Phenotype: cold sensitive on minimal medium

This may be a double mutant of the original cr4 (see CC-33) with another mutation that affects chloroplast ribosome biogenesis.

Elizabeth Harris, Boynton-Gillham laboratory, Duke University, 1983

Phenotype: cold sensitive on minimal medium; inhibitor resistant (pyrithiamine)

Please see CC-33 for more information on the cr4 mutant, which is deficient in chloroplast ribosomes. This strain was derived from the following series of crosses, which were carried out in an unsuccessful attempt to map this mutation.

CC-33 cr4 mt+ x CC-1144 sr1 msr1 nr1 act2 pyr1 NIT+ mt-

A product from this cross was then crossed to CC-1256 pyr1 ery3 sr1 act-2 mt+.

  • Locus:
  • PYR1
  • Chromosome:
  • 4

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires thiamine

From a cross in 1983 of wild type 21 gr (NIT+) x a mt- strain ac17 thi2. This strain has the wild type alleles at the NIT1 and NIT2 loci and can grow on nitrate.

The thi2 mutation is best scored by spot-testing on minimal medium. It may be necessary to repeat the test to get good results. Please see CC-24 for more information on this mutation.

  • Locus:
  • THI2
  • Chromosome:
  • 3

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate; antibiotic resistant (erythromycin)

From CC-1156 ac115 x CC-1105 ery3 msr1 sr1, 1982

This strain provides two easily scored markers on the right arm of linkage group I.

  • Locus:
  • AC115, ERY3
  • Chromosome:
  • 1,8

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: cold sensitive on minimal medium; inhibitor resistant (methionine sulfoximine); yellow in the dark

From CC-1366 ac20 y6 x CC-1105 ery3 msr1 sr1, 1982

This is a triply-marked strain for analysis of linkage group I. The y6 marker is closely linked to the centromere, the ac20 and msr1 markers are on the right arm [order y6 — ac20 – msr1].

In combination, y6 and ac20 produce cells with an intense yellow color when grown in the dark. Scoring ac20 for acetate requirement on minimal medium works best at low temperature (12-15 degrees C).

  • Locus:
  • AC20, MSR1, Y6
  • Chromosome:
  • 1

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate

From CC-1035 pf17 x CC-1 ac5 (no longer in the collection), selected for pale green color. This strain does not carry the pf17 mutation.

The ac5 phenotype is easier to score on Tris-minimal than HS medium.

Please see CC-517 for more information on ac5.

  • Locus:
  • AC5
  • Chromosome:
  • 7

Chlamydomonas Genetics Center, Duke University, 1983

From CC-802 lf1 mt+ x CC-124 wild type, 1982.

The lf1 mutant has long flagella and is very slow to regenerate after deflagellation. Please see CC-802 for more information on this mutation.

  • Locus:
  • LF1
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate; antibiotic resistant (cycloheximide)

From CC-1589 act1 x CC-544 ac80, 1983

This strain provides two markers on linkage group II.

  • Locus:
  • AC80, ACT1
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires nicotinamide; motility impaired

From CC-1111 nic15 pf9 x CC-40 cr1 (neither of these strains is still in the collection).

This strain was derived from a mapping cross for markers on linkage group XII/XIII. The nic pf combination is preserved in the opposite mating type, but this strain does not contain the cr1 mutation.

For more information on nic15, please see CC-14, and for pf9 please see CC-1254.

  • Locus:
  • NIC15 [ASO1], PF9 [DHC1]
  • Chromosome:
  • 12

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate

The ac145 mutation was UV-induced in Levine’s laboratory, and was described as non-photosynthetic, deficient in “component X.”

This strain is from CC-864 nic13 x CC-571 ac145 (no longer in the collection), 1983, and has the wild type allele at the NIC13 locus.

The stock of this mutant (CC-571) obtained from Levine’s laboratory in 1978 by the Chlamydomonas Genetics Center showed a leaky acetate requirement but yielded clean isolates on crossing to wild type strains. The CC-1683 and CC-1684 derivatives were clearly acetate-requiring at the time they were created, but now are more difficult to score. Good isolates could probably be recovered again by crossing.

At one time AC145 was a key marker for linkage group X, but has become less important as molecular markers for this group have been characterized.

Levine RP, Volkmann D (1961) Mutants with impaired photosynthesis in Chlamydomonas reinhardi. Biochem. Biophys Res Commun 6:264-269

Goodenough UW, Levine RP (1969) Chloroplast Ultrastructure in Mutant Strains of Chlamydomonas reinhardi Lacking Components of the Photosynthetic Apparatus. Plant Physiol 44:990-1000

Levine RP, Goodenough UW 1970) The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi. Annu Rev Genet 4:397-408

  • Locus:
  • AC145
  • Chromosome:
  • 10