Strains

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: wall deficient

From CC-851 cw2 mt+ x CC-124 wild type mt-

This cross was made to get a cw2 strain with better viability in crosses than the parent CC-851. It’s been reported that cw2 has a cleaner surface, with fewer adherent cell wall remnants, than cw15.

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: wall deficient

From CC-860 cw3 mt+ x CC-124 wild type mt-

This cross was made to get a cw3 strain with better viability in crosses than the parent CC-860.

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: antibiotic resistant (erythromycin); motility impaired

From a series of crosses; pf22 comes from CC-1125, which is no longer in the collection; ery3b derives from CC-78.

This strain provides markers that span the centromere on linkage group I. See CC-1709 for the same combination in mating type minus, and CC-2495 for more information on the PF22 locus.

  • Locus:
  • ERY3, PF22
  • Chromosome:
  • 1

Chlamydomonas Genetics Center, Duke University, 1983

Phenotype: requires acetate; yellow in the dark

From CC-1173 y7 x a subclone of CC-1138 ac17 thi2 (nit+) mt- (which is no longer in the collection).

  • Locus:
  • AC17, Y7
  • Chromosome:
  • 3

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-134 a10-5A+

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-140 a10-8A+

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 6:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-165 a6-4A+. Induced by 5-fluorodeoxyuridine plus ethyl methanesulfonate. This is a nuclear mutation but has not been mapped.

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 6:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-172 a6-4D-

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-176 12CA+

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-177 12CB+

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

From Laurens Mets, University of Chicago, April 1984

Phenotype: requires acetate

Received as Spreitzer and Mets III-178 12CC+

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

Chlamydomonas Genetics Center, Duke University, 1984

Phenotype: requires acetate

From CC-125 wild type x CC-1380 ac213 mt-

This mutation is closely linked to mating type. The isolate that became CC-1754 was the only acetate product recovered in mating type plus from this cross.

  • Locus:
  • AC213
  • Chromosome:
  • 6

Boynton-Gillham laboratory, Duke University, 1984

Phenotype: antibiotic resistant (kirromycin)

From a mutagenesis of FuDR-treated wild type CC-125 in 1983.

This is a uniparentally inherited kirromycin resistant mutant that is thought to map to the chloroplast tufA gene, encoding elongation factor EF-Tu, encoding elongation factor EF-Tu.

  • Locus:
  • tufA
  • Chromosome:
  • chloroplast

Chlamydomonas Genetics Center, Duke University, 1984

Phenotype: impaired motility; cannot use glutamine as N source

From a cross of CC-1128 pf27 (no longer in the collection) to a gln1 strain obtained from Bob Hodson.

This strain derives from the cross that placed gln1 on linkage group XII/XIII. Subsequent crosses established that it was on the right arm of this linkage group.

The gln mutant can be scored by inability to grow on glutamine as sole nitrogen source. The test agar can be prepared using standard recipies, omitting NH4Cl and adding 0.292 g glutamine in 5 ml sterile water per liter, after autoclaving. Best results will be obtained if highly purified agar is used, e.g. Difco Noble agar.

For more information on pf27, please see CC-1387.

  • Locus:
  • GLN1, PF27
  • Chromosome:
  • 12

Elizabeth Harris, Boynton-Gillham laboratory, Duke University, 1984

Phenotype: cold sensitive on minimal medium

This isolate was obtained from a cross of CC-125 x CC-252 cr6 mt-.

Chlamydomonas Genetics Center, Duke University, 1984

Phenotype: cold sensitive on minimal medium; antibiotic and inhibitor resistant (erythromycin, methionine sulfoximine, streptomycin)

This strain came from a cross of CC-251 cr6 mt+ to the multiply marked strain CC-1105.

  • Locus:
  • ERY3, MSR1, SR1
  • Chromosome:
  • 1,9

From Laurie Mets, April 1984, as III 130 10-6c; this is the original chloroplast Rubisco mutant 10-6C of Spreitzer and Mets.

Phenotype: requires acetate

This is a point mutation in the chloroplast rbcL gene (Gly to Asp at 171) that results in a Rubisco large subunit with altered isoelectric point.

This strain is light-sensitive and should be maintained in very dim light or in total darkness.

Spreitzer, Mets (1980) Non-mendelian mutation affecting ribulose-1,5-biphosphate carboxylase structure and activity. Nature 285:114-115

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

Dron M, Rahire M, Rochaix JD, Mets L (1983) First DNA sequence of a chloroplast mutation: a missense alteration in the ribulosebisphosphate carboxylase large subunit gene. Plasmid 9:321-324

Spreitzer RJ, Ogren WL (1983) Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 80:6293-6297

Gans P, Rebeille F (1988) Light inhibition of mitochondrial respiration in a mutant of Chlamydomonas reinhardtii devoid of ribulose-1,5-bisphosphate carboxylase/oxygenase activity. Arch Biochem Biophys 260:109-117

Rawat M, Henk MC, Lavigne LL, Moroney JV (1996) Chlamydomonas reinhardtii mutants without ribulose-1,5-bisphosphate carboxylase-oxygenase lack a detectable pyrenoid. Planta 198:263-270

  • Locus:
  • rbcL
  • Chromosome:
  • chloroplast

From the University of Texas Algal Collection (UTEX), April 1984

This is also known as C. moewusii S-16.

Guillard RRL (1960) A Mutant of Chlamydomonas moewusii lacking contractile vacuoles. Protozoology 7:262-268

Chlamydomonas Genetics Center, Duke University, 1984

Phenotype: wall deficient

From CC-425 cw15 arg2 sr-u-2-60 mt+ x CC-1736 cw15 arg7 mt- (no longer in the collection)

This strain was constructed in response to a request for cw15 in a diploid background.

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-1 mutation was induced by Loppes using ethyl methanesulfonate.

This is a non-complementing but perhaps slightly leaky mutation at the ARG7 locus.

The following citations refer specifically to arg7-1:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-2 mutation was induced by Loppes using ethyl methanesulfonate.

This allele can complement with the original arg7, arg7-5, arg7-8 (aka arg2), and arg7-13. It cannot complement with arg7-3, arg7-4, arg7-7 or arg7-12. It seems to be stable in culture.

The following citations refer specifically to arg7-2:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-3 mutation was induced by Loppes using ethyl methanesulfonate.

This allele can complement with the original arg7, arg7-5, arg7-8 (aka arg2), and arg7-13. It cannot complement with arg7-2, arg7-4, arg7-7 or arg7-12. It was described as non-reverting, and seems to be stable in culture.

The following citations refer specifically to arg7-3:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Konvalinkova V, Matagne RF, Loppes R (1974) Iniduction and analysis of revertants from various arg-7 mutants lacking argininosuccinate lyase in Chlamydomonas reinhardi. Mutat Res 24:69-72

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-4 mutation was induced by Loppes using ethyl methanesulfonate.

This is a stable, clean arg7 allele, probably a deletion since it fails to complement with either arg7-13 or the cluster comprising arg7-2, arg7-3, arg7-7 and arg7-12. It does supposedly complement with arg7-8 (aka arg2). We don’t know whether it can be transformed with the ARG7 plasmid.

The following citations refer specifically to arg7-4:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-5 mutation was UV-induced by Loppes.

This allele complements with arg7-2, arg7-3, arg7-4, arg7-7, arg7-8, arg7-12, and arg7-13 in diploids, but does not complement with the original arg7 mutant.

The following citations refer specifically to arg7-5:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-6 mutation was induced by Loppes using nitrosoguanidine.

This allele does not complement or recombine with other arg7 alleles in diploids.

The following citations refer specifically to arg7-6:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-7 mutation was induced by Loppes using nitrosoguanidine.

This allele can complement arg7, arg7-5, arg7-8, and arg7-13 in diploids; does not complement arg7-2, arg7-3, arg7-4, or arg7-12

The following citations refer specifically to arg7-7:

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R, Stijkert PJ (1972) Arginine metabolism in Chlamydomonas reinhardi. Conditional expression of arginine-requiring mutants. Mol Gen Genet 116:248-257

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

Also known as arg2, this is a relatively stable allele at the ARG7 locus. It complements with the original arg7, arg7-2, arg7-3, arg7-4, arg7-5, arg7-7, arg7-12, and arg7-13, and can be transformed with the ARG7 plasmid. Please see CC-48 for more information on this mutant.

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-9 mutation was induced by Matagne using nitrosoguanidine.

This allele does not complement or recombine with other arg7 alleles in diploids.

The following citation refers specifically to arg7-9:

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-10 mutation was induced by Matagne using nitrosoguanidine.

This is a stable, non-complementing allele. We don’t know whether it is transformable with the ARG7 plasmid.

The following citation refers specifically to arg7-10:

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Rene Matagne, University of Liege, June 1984

Phenotype: requires arginine

The arg7-11 mutation was induced by Matagne using nitrosoguanidine.

This is a stable, non-complementing allele. We don’t know whether it is transformable with the ARG7 plasmid.

The following citation refers specifically to arg7-11:

Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99

  • Locus:
  • ARG7
  • Chromosome:
  • 1