Strains
CC-1830 arg7-12 mt-
$30.00
$30.00
From Rene Matagne, University of Liege, June 1984
Phenotype: requires arginine
The arg7-12 mutation was induced by Matagne using nitrosoguanidine.
This allele can complement with the original arg7, arg7-5, arg7-8 (aka arg2), and arg7-13. It cannot complement with arg7-2, arg7-3, arg7-4, or arg7-7. It seems to be stable.
The following citation refers specifically to arg7-12:
Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99
CC-1831 arg7-13 mt-
$30.00
$30.00
From Rene Matagne, University of Liege, June 1984
Phenotype: requires arginine
The arg7-13 mutation was induced by Matagne using nitrosoguanidine.
This allele complements with the original arg7, arg7-2, arg7-3, arg7-5, arg7-8, and arg7-12.
The following citation refers specifically to arg7-13:
Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99
Matagne RF (1978) Fine structure of the arg-7 ciston in chlamydomonas reinhardi. Complementation between arg-7 mutants defective in argininosuccinate lyase. Mol Gen Genet 160:95-99
CC-1832 P2 mt+
$30.00
$30.00
From Rene Matagne, University of Liege, June 1984
Phenotype: altered phosphate metabolism
This mutant lacks bound acid phosphatase.
Loppes R, Matagne RF (1973) Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis. Genetics 75, 593-604
Matagne RF, Loppes R, Deltour R (1976) Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants. J Bacteriol 126:937-950
CC-1833 Pa mt+
$30.00
$30.00
From Rene Matagne, University of Liege, June 1984
Phenotype: altered phosphate metabolism
This strain lacks soluble acid phosphatase.
Loppes R, Matagne RF (1973) Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis. Genetics 75:593-604
Matagne RF, Loppes R, Deltour R (1976) Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants. J Bacteriol 126:937-950
CC-1834 PD2 mt-
$30.00
$30.00
From Rene Matagne, University of Liege, June 1984
Phenotype: altered phosphate metabolism
This strain lacks derepressible neutral phosphatase.
Loppes R, Matagne RF (1973) Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis. Genetics 75:593-604
Matagne RF, Loppes R, Deltour R (1976) Phosphatase of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants. J Bacteriol 126:937-950
Dumont F, Loppes R, Kremers P (1990) New polypeptides and in-vitro-translatable mRNAs are produced by phosphate-starved cells of the unicellular algaChlamydomonas reinhardtii. Planta 182:610-616
From Rene Matagne, University of Liege, June 1984
This is supposedly a mt+/mt- diploid strain generated by somatic fusion, but in 2002 Olivier Vallon reported that it mates as plus rather than the expected minus.
Matagne RF, Beckers MC (1983) Perturbation of chloroplast gene transmission in diploid and triploid zygotes of Chlamydomonas reinhardi by 5-fluorodeoxyuridine. Curr Genet 7:335-338
From Rene Matagne, University of Liege, June 1984
This is a mt+/mt+ diploid strain generated by somatic fusion.
Matagne RF, Beckers MC (1983) Perturbation of chloroplast gene transmission in diploid and triploid zygotes of Chlamydomonas reinhardi by 5-fluorodeoxyuridine. Curr Genet 7:335-338
From Rene Matagne, University of Liege, June 1984
This is supposedly a mt-/mt- diploid strain generated by somatic fusion. but in 2002 Olivier Vallon reported that it mates as plus rather than the expected minus..
Matagne RF, Beckers MC (1983) Perturbation of chloroplast gene transmission in diploid and triploid zygotes of Chlamydomonas reinhardi by 5-fluorodeoxyuridine. Curr Genet 7:335-338
CC-1838 C. moewusii fg-1
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was UV-induced in the paralyzed mutant M.475. It lacks flagellar gliding movement on solid substrata.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38:348-349
CC-1839 C. moewusii fg-2
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was isolated by Lewin and Burrascano. It lacks gliding motility on the flagellar surface.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38:348-349
Reinhart FD, Bloodgood RA (1988) Gliding defective mutant cell lines of Chlamydomonas moewusii exhibit alterations in a 240 kDa surface-exposed flagellar glycoprotein. Protoplasma 144:110-118
CC-1840 C. moewusii fg-3
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was isolated by Lewin and Burrascano. It lacks gliding motility on the flagellar surface.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38:348-349
Reinhart FD, Bloodgood RA (1988) Gliding defective mutant cell lines of Chlamydomonas moewusii exhibit alterations in a 240 kDa surface-exposed flagellar glycoprotein. Protoplasma 144:110-118
CC-1841 C. moewusii fg-5
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was isolated by Lewin and Burrascano. It lacks gliding motility on the flagellar surface.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38:348-349
CC-1842 C. moewusii fg-6
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was isolated by Lewin and Burrascano. It lacks gliding motility on the flagellar surface.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38:348-349
CC-1843 C. moewusii fg-7
$30.00
$30.00
From Robert Bloodgood, University of Virginia, June 1984
This mutant was isolated by Lewin and Burrascano. It lacks gliding motility on the flagellar surface.
Lewin RA (1982) A new kind of motility mutant (non-gliding) in Chlamydomonas. Experientia 38, 348-349
Reinhart FD, Bloodgood RA (1988) Gliding defective mutant cell lines of Chlamydomonas moewusii exhibit alterations in a 240 kDa surface-exposed flagellar glycoprotein. Protoplasma 144:110-118
From Ruth Galloway, Goodenough lab, Washington University, June 1984
Phenotype: herbicide resistant (bromacil)
This is a chloroplast mutant resistant to bromacil, slightly cross-resistant to DCMU.
Galloway RE, Mets LJ (1984) Atrazine, bromacil, and diuron resistance in chlamydomonas: a single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol 74:469-474
Govindjee, Eggenberg P, Pfister K, Strasser RJ (1992) Chlorophyll a fluorescence decay in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii and the formate effect. Biochim Biophys Acta 1101:353-358
From Ruth Galloway, Goodenough lab, Washington University, June 1984
Phenotype: herbicide resistant (atrazine)
This is a chloroplast mutant resistant to atrazine, but not cross-resistant to bromacil. The mutation was isolated by Galloway and Mets in strain 2137 (see CC-3269). The atrazine resistance results from a Phe to Tyr change at amino acid residue 255 in the D1 protein.
Galloway RE, Mets LJ (1984) Atrazine, bromacil, and diuron resistance in chlamydomonas: a single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol 74:469-474
Erickson JM, Rahire M, Rochaix JD, Mets L (1985) Herbicide resistance and cross-resistance: changes at three distinct sites in the herbicide-binding protein. Science 228:204-207
Govindjee, Eggenberg P, Pfister K, Strasser RJ (1992) Chlorophyll a fluorescence decay in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii and the formate effect. Biochim Biophys Acta 1101:353-358
From Ruth Galloway, Goodenough lab, Washington University, June 1984
Phenotype: herbicide resistant (atrazine)
This is a chloroplast mutant resistant to atrazine, but not cross-resistant to bromacil. The mutation was isolated by Galloway and Mets in strain 2137 (see CC-3269). The atrazine resistance results from a Phe to Tyr change at amino acid residue 255 in the D1 protein.
Galloway RE, Mets LJ (1984) Atrazine, bromacil, and diuron resistance in chlamydomonas: a single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol 74:469-474
Erickson JM, Rahire M, Rochaix JD, Mets L (1985) Herbicide resistance and cross-resistance: changes at three distinct sites in the herbicide-binding protein. Science 228:204-207
Govindjee, Eggenberg P, Pfister K, Strasser RJ (1992) Chlorophyll a fluorescence decay in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii and the formate effect. Biochim Biophys Acta 1101:353-358
Boynton-Gillham laboratory, Duke University, 1984
From a cross by Nicholas Gillham, CC-1373 C. smithii mt+ x CC-124 wild type C. reinhardtii mt- [treated with acriflavine to eliminate its mitochondrial genome].
This strain has the chloroplast and mitochondrial genomes of the CC-1373 parent, in what is presumed to be a 50-50 hybrid nuclear background.
Boynton JE, Harris EH, Burkhart BD, Lamerson PM, Gillham NW (1987) Transmission of mitochondrial and chloroplast genomes in crosses of Chlamydomonas. Proc Natl Acad Sci U S A 84:2391-2395
CC-1859 ca1 pmp1 mt+
$30.00
$30.00
From Martin Spalding, Iowa State University, October 1984
Phenotype: altered CO2 assimilation
This strain requires high CO2 concentrations for phototrophic growth. We maintain this strain in dim light.
Originally pmp-1-16-5k, this mutant was induced by 5-fluorodeoxyuridine plus ethyl methanesulfonate in the 2137 strain (see CC-3269). The pmp1 mutant is defective in inorganic carbon transport, and requires high CO2 concentrations for phototrophic growth. The pmp1 and ad (air-dier) mutants are alleles at the LCIB locus.
For more information on the ca1 mutation, which is in the CAH3 gene, please see CC-1219, and for reference citations for pmp1, please see CC-1860.
CC-1860 pmp1 mt+
$30.00
$30.00
From Martin Spalding, Iowa State University, October 1984
Phenotype: altered CO2 assimilation
Originally pmp-1-16-5k, this mutant was induced by 5-fluorodeoxyuridine plus ethyl methanesulfonate in the 2137 strain (see CC-3269). The pmp1 mutant is defective in inorganic carbon transport, and requires high CO2 concentrations for phototrophic growth. The pmp1 and ad (air-dier) mutants are alleles at the LCIB locus.
We maintain this strain in dim light.
Spalding MH, Spreitzer RJ, Ogren WL (1983) Reduced Inorganic Carbon Transport in a CO(2)-Requiring Mutant of Chlamydomonas reinhardii. Plant Physiol 73:273-276
Spalding MH, Spreitzer RJ, Ogren WL (1983) Genetic and physiological analysis of the CO2-concentrating system of Chlamydomonas reinhardii. Planta 159:261-266
Spalding MH, Ogren WL (1985) CO2 exchange characteristics during dark-light transitions in wild-type and mutant Chlamydomonas reinhardii cells. Photosynth Res 6:363-369
Moroney JV, Tolbert NE, Sears BB (1986) Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii. Mol Gen Genet 204:199-203
Wang Y, Spalding MH (2006) An inorganic carbon transport system responsible for acclimation specific to air levels of CO2 in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 103:10110-10115
From Ruth Galloway, Goodenough lab, Washington University, January 1985
Phenotype: sex reversal; inhibitor resistant (fluoroacetamide)
The imp11 mutation in mating type minus causes gametes to agglutinate as if they were mating type plus (for more information, please see CC-1148). The famr7 mutation, which was described by Hodson and Gresshoff, confers resistance to fluoroacetamide.
Hodson RC, Gresshoff PM (1987) Fluoroacetamide resistance mutations in Chlamydomonas reinhardtii. Arch Microbiol 148:8-13
CC-1865 imp1 (fus1) arg2 mt+
$30.00
$30.00
From Ruth Galloway, Goodenough lab, Washington University, January 1985
Phenotype: does not mate
This is the imp1 sexual fusion mutant in an arginine-requiring background. For more information on this locus, please see CC-1158.
From Ruth Galloway, Goodenough lab, Washington University, January 1985
Phenotype: does not mate: requires arginine
Please see CC-1147 for more information on the imp10 mutation.
Hwang CJ, Monk BC, Goodenough UW (1981) Linkage of Mutations Affecting minus Flagellar Membrane Agglutinability to the mt Mating-Type Locus of Chlamydomonas. Genetics 99:41-47
Galloway RE, Goodenough UW (1985) Genetic analysis of mating locus linked mutations in Chlamydomonas reinhardii. Genetics 111:447-461
Saito T, Small L, Goodenough UW (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat. J Cell Biol 122:137-147
Ferris PJ, Goodenough UW (1994) The mating-type locus of Chlamydomonas reinhardtii contains highly rearranged DNA sequences. Cell 76:1135-1145
CC-1868 imp1 (fus1) arg7 mt+
$30.00
$30.00
From Ruth Galloway, Goodenough lab, Washington University, January 1985
Phenotype: does not mate; requires arginine
This is the imp1 sexual fusion mutant in an arginine-requiring background. For more information on the imp1 mutation, please see CC-1158.
Sammlung von Algenkulturen (SAG), January 1985
This is a mutant with a hatching deficiency, isolated by U.G. Schlösser. It has not been analyzed genetically.
From Robert Lee, Dalhousie University, February 1985
This is a Lee’s stock of the sr-2 chloroplast mutant resistant to 500 micrograms/ml streptomycin. The mutation was used extensively in studies by Lemieux and others on inheritance of chloroplast genes in crosses of C. eugametos x C. moewusii.
McBride AC, McBride JC (1975) Uniparental inheritance in Chlamydomonas eugametos (chlorophyceae). J Phycol 11:343-344
Lemieux C, Turmel M, Lee RW (1980) Characterization of chloroplast DNA in Chlamydomonas eugametos and C. moewusii and its inheritance in hybrid progeny. Curr Genet 2:139-147
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) Chloroplast DNA recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonmendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc Natl Acad Sci U S A 81:1164-1168
From Robert Lee, Dalhousie University, February 1985
This is a mutation in the chloroplast 16S ribosomal RNA of C. moewusii, conferring resistance to 250 micrograms/ml streptomycin.
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) Chloroplast DNA recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonmendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc Natl Acad Sci U S A 81:1164-1168
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) A genetical approach to the physical mapping of chloroplast genes in Chlamydomonas. Can J Biochem Cell Biol 62:225-229
From Robert Lee, Dalhousie University, February 1985
This is a mutation in the chloroplast 23S ribosomal RNA of C. moewusii, conferring resistance to 400 micrograms/ml streptomycin.
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) Chloroplast DNA recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonmendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc Natl Acad Sci U S A 81:1164-1168
Lemieux C, Turmel M, Seligy VL, Lee RW (1984) A genetical approach to the physical mapping of chloroplast genes in Chlamydomonas. Can J Biochem Cell Biol 62:225-229
CC-1877 pf28 mt-
$30.00
$30.00
From David Mitchell, April 1985
Phenotype: impaired motility
The pf28 mutant was isolated in Rosenbaum’s laboratory and was subsequently characterized by Kamiya and others. It is an allele at the ODA2 locus, which corresponds to the gene encoding the outer arm dynein gamma heavy chain. It swims slowly and is defective in backward swimming.
Mitchell DR, Rosenbaum JL (1985) A motile Chlamydomonas flagellar mutant that lacks outer dynein arms. J Cell Biol 100:1228-34
Brokaw CJ, Kamiya R (1987) Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function. Cell Motil Cytoskeleton 8:68-75
Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii. J Cell Biol 107:2253-2258
Rupp G, O'Toole E, Gardner LC, Mitchell BF, Porter ME (1996) The sup-pf-2 mutations of Chlamydomonas alter the activity of the outer dynein arms by modification of the gamma-dynein heavy chain. J Cell Biol 135:1853-1865
Ahmed NT, Gao C, Lucker BF, Cole DG, Mitchell DR (2008) ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. J Cell Biol 183:313-322
CC-1883 cw15 NIT+ mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University, 1985
Phenotype: wall deficient
From CC-1690 21 gr mt+ x CC-1615 cw15 nit2 mt-; the cw15 mutation ultimately derives from CC-400.
This is the widely-used wall deficient mutant cw15 in a strain that can grow on nitrate.
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