Strains
From the University of Texas Algal Collection (UTEX), May 1985, as 478W spherical
This mutant was UV-induced by Lewin, his M.478. The cells are small and spherical, and grow slowly.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
From the University of Texas Algal Collection (UTEX), May 1985, as 470 twins/monsters
This mutant was UV-induced by Lewin, his M.470. It was described as having aberrant cell division, such that longitudinal division was arrested before completion, leaving pairs of cells still attached to one another. This appears to be an allele at the same locus as M.202 (CC-1886), which has a somewhat different phenotype.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
From the University of Texas Algal Collection (UTEX), May 1985, as 202 mt+ monsters
This mutant was UV-induced by Lewin, his M.202. It was described as having aberrant cell division, producing grossly misshapen cells. This appears to be an allele at the same locus as M.470 (CC-1885), which has a somewhat different phenotype.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
From the University of Texas Algal Collection (UTEX), May 1985
This strain was isolated from soil from a rice field in Sasayama, Hyogo Prefecture, Japan, in 1952. Tsubo identified it as a variety of C. moewusii. Both mating types were recovered from the original soil sample, and mating was obtained. However, Tsubo was unable to obtain agglutination or fusion with the principal laboratory strains of either C. moewusii or C. eugametos. Wiese and Wiese confirmed this lack of interfertility, and assigned the var. rotunda strains the designation of C. moewusii syngen III.
Tsubo Y (1961) Chemotaxis and Sexual Behavior in Chlamydomonas. J Protozool 8:114-121
Wiese L, Wiese W (1977) On Speciation by Evolution of Gametic Incompatibility: A Model Case in Chlamydomonas Amer. Nat 111:733-742
From the University of Texas Algal Collection (UTEX), May 1985, as 1017 paralyzed mt+
This is a motility mutant isolated by Lewin, his M.1017.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985, as 1021 paralyzed mt+
This is a motility mutant isolated by Lewin, his M.1021.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985, as 1022 paralyzed mt+
This is a motility mutant isolated by Lewin, his M.1022.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985, as 336 paralyzed, B1 auxotroph mt+
This is Lewin strain M.336, a UV-induced thiamine-requiring mutant in the backgroup of the paralyzed strain M.236. It can grow with thiazole and the pyrimidine moiety of thiamine.
Lewin RA (1952b) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1953a) The genetics of Chlamydomonas moewusii Gerloff. J Genet 51:543-560
From the University of Texas Algal Collection (UTEX), May 1985, as 1034 paralyzed mt+
This is a motility mutant isolated by Lewin, his M.1034.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985, as 1038 paralyzed mt+
This is a motility mutant isolated by Lewin, his M.1038.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1954) Mutants of Chlamydomonas moewusii with impaired motility. J Gen Microbiol 11:358-363
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985, as 470 x 1002 paralyzed, twins mt+
This is Lewin’s twinning mutant M.470 (CC-1885) coupled with the motility mutant M.1002.
Lewin RA (1952) Ultraviolet induced mutations in Chlamydomonas moewusii Gerloff. J Gen Microbiol 6:233-248
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the University of Texas Algal Collection (UTEX), May 1985
This strain was isolated from soil collected in 1957 on Yap, in the Caroline Islands, by Ralph Lewin, and is not cross-fertile with other isolates of C. moewusii, Wiese and Wiese referred to this strain and its opposite mating type (UTEX 793, not in our collection) as C. moewusii syngen II. Based on patterns of sensitivity to glycosidases and various proteolytic enzymes, Wiese et al. inferred their probable equivalence to the conventional designations for mating type in other C. moewusii isolates.
Wiese L, Wiese W (1977) On speciation by evolution of gametic Incompatibility: a model case in Chlamydomonas. Amer Nat 111:733-742
Wiese L, Laramie A, Baker W, Baker DL (1983) A General and Fundamental Molecular Bipolarity of the Sex Cell Contact Mechanism as Revealed by Tunicamycin and Bacitracin in Chlamydomonas. Amer Nat 122:806-816
From the University of Texas Algal Collection (UTEX), May 1985
This strain was isolated from a rice field in Hassai, Hyogo prefecture, Japan, in 1958, and described by Tsubo. It is not interfertile with other isolates of C. moewusii. Wiese and Wiese referred to this strain and its opposite mating type as C. moewusii syngen III.
Tsubo Y (1961) Chemotaxis and Sexual Behavior in Chlamydomonas. J Protozool 8:114-121
Wiese L, Wiese W (1977) On Speciation by Evolution of Gametic Incompatibility: A Model Case in Chlamydomonas. Amer Nat 111:733-742
From the University of Texas Algal Collection (UTEX), May 1985
This strain was isolated from a rice field in Hassai, Hyogo prefecture, Japan, in 1958, and described by Tsubo. It is not interfertile with other isolates of C. moewusii. Wiese and Wiese referred to this strain and its opposite mating type as C. moewusii syngen III.
Tsubo Y (1961) Chemotaxis and Sexual Behavior in Chlamydomonas. J Protozool 8:114-121
Wiese L, Wiese W (1977) On Speciation by Evolution of Gametic Incompatibility: A Model Case in Chlamydomonas. Amer Nat 111:733-742
From the University of Texas Algal Collection (UTEX), May 1985
This strain of C. moewusii was isolated from a roadside ditch in Iowa by G.M. Smith in 1950. It was characterized by Hoshaw, who reviewed Smith’s collection of Chlamydomonas isolates in 1965.
Hoshaw RW (1965) Mating types of Chlamydomonas from the collection of Gilbert M. Smith. J Phycol 1:194-196
From the University of Texas Algal Collection (UTEX), May 1985
This strain of C. moewusii, which is not interfertile with the laboratory strains, was isolated in Japan by T. Majima and K. Iwasa.
Wiese L, Wiese W (1977) On Speciation by Evolution of Gametic Incompatibility: A Model Case in Chlamydomonas. Amer Nat 111:733-742
From the University of Texas Algal Collection (UTEX), May 1985
This strain of C. moewusii, which is not interfertile with the laboratory strains, was isolated in Japan by T. Majima and K. Iwasa.
Wiese L, Wiese W (1977) On Speciation by Evolution of Gametic Incompatibility: A Model Case in Chlamydomonas. Amer Nat 111:733-742
From the University of Texas Algal Collection (UTEX), May 1985
This is a homothallic strain of C. moewusii, isolated from the Black Warrior River in Alabama in 1973.
Deason TR, Ratnasabapathy M (1976) A New Homothallic Variety of Chlamydomonas Moewush (Chlorophyceae). J Phycol 12:82-85
From the University of Texas Algal Collection (UTEX), May 1985
We are not certain if this is the same as gf-1 (CC-1371) or fg-1 (CC-1838), or if it is some other non-gliding mutant.
CC-1910 ac55 cw15 mt-
$30.00
$30.00
Chlamydomonas Genetics Center, Duke University, 1985
Phenotype: wall deficient; somewhat chlorophyll deficient
From CC-564 ac55 x CC-1615 cw15
This strain was created to get a cw15 strain with an obvious color marker. The ac55 mutant tends to be paler than wild type, but is not stringently acetate-requiring.
CC-1911 FUD944 (atpB) mt+
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, to Boynton-Gillham laboratory, Duke University, June 1985
Phenotype: requires acetate
This is a mutation in the chloroplast atpB gene.
CC-1917 enh1 mt+
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
The enh1 mutation has no phenotype in a wild type background, but modifies the uni1 mutation so that the population mostly lacks flagella rather than being uniflagellate.
Ramanis Z, Luck DJ (1986) Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 83:423-426
Dutcher SK (1986) Genetic properties of linkage group XIX in Chlamydomonas reinhardtii. Basic Life Sci 40:303-325
CC-1918 fla9 mt-
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The fla9 mutant, which was induced with nitrosoguanidine in Luck’s laboratory, cannot regenerate flagella at 32 degrees. Huang et al. reported linkage between fla9 and the uni1 mutant on linkage group XIX, but it does not appear on subsequent maps.
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of Chlamydomonas Reinhardtii. Genetics 100:579-586
Huang B, Ramanis Z, Dutcher SK, Luck DJ (1982) Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29:745-753
CC-1919 fla10-1 mt-
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The fla10 mutant cannot regenerate flagella at 32 degrees. The original fla10 allele (fla10-1) is a C to A transversion that alters amino acid 329 in the motor domain of the kinesin-homologous KHP1 protein. Other alleles at this locus include the null murant fla10-2 mutant (CC-4180), and fla10-14 (CC-1395).
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85
Walther Z, Vashishtha M, Hall JL (1994) The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein. J Cell Biol 126:175-188
Kozminski KG, Beech PL, Rosenbaum JL (1995) The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane. J Cell Biol 131:1517-1527
Piperno G, Mead K, Henderson S (1996) Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas. J Cell Biol 133:371-379
Vashishtha M, Walther Z, Hall JL (1996) The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. J Cell Sci 109:541-549
Cole DG, Diener DR, Himelblau AL, Beech PL, Fuster JC, Rosenbaum JL (1998) Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons. J Cell Biol 141:993-1008
Matsuura K, Lefebvre PA, Kamiya R, Hirono M (2002) Kinesin-II is not essential for mitosis and cell growth in Chlamydomonas. Cell Motil Cytoskeleton 52:195-201
Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341-1354
Miller MS, Esparza JM, Lippa AM, Lux FG 3rd, Cole DG, Dutcher SK (2005) Mutant kinesin-2 motor subunits increase chromosome loss. Mol Biol Cell 16:3810-3820
CC-1920 fla11 mt-
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The fla11 mutant slowly resorbs flagella at 32 degrees and is slow to generate flagella when induced to form gametes at 21 degrees. Pedersen et al. determined that this is a point mutation in the gene encoding the IFT172 protein, resulting in a Leu to Pro change at amino acid residue 1615.
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of Chlamydomonas Reinhardtii. Genetics 100:579-586
Pedersen LB, Miller MS, Geimer S, Leitch JM, Rosenbaum JL, Cole DG (2005) Chlamydomonas IFT172 is encoded by FLA11, interacts with CrEB1, and regulates IFT at the flagellar tip. Curr Biol 15:262-266
CC-1921 fla12 mt-
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The fla12 mutant slowly resorbs its flagella at 32 degrees, and produces flagella in about 2 hours when induced to form gametes at 21 degrees. Iomini et al. suggest the FLA12 locus may be involved in intraflagellar transport.
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII. Genetics 100:579-586
Ramanis Z, Luck DJ (1986) Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 83:423-426
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24
CC-1922 fla13 mt-
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The original fla13 mutant slowly resorbed its flagella at 32 degrees, and produced flagella in about 2 hours when induced to form gametes at 21 degrees. Iomini et al. reported in 2001 that their isolate of this mutant had lost the original phenotype.
Adams GM, Huang B, Luck DJ (1982) Temperature-Sensitive, Assembly-Defective Flagella Mutants of CHLAMYDOMONAS REINHARDTII. Genetics 100:579-586
Ramanis Z, Luck DJ (1986) Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 83:423-426
Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24
CC-1923 pf10.29A mt+
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
We are not certain if this is the same allele as CC-899 pf10 mt-.
CC-1924 pf29 mt+
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
Cells of the pf29 mutant move slowly, in jerky, linear paths.
This mutant was mapped in Luck’s laboratory to the UNI linkage group (XIX, now chromosome 17), but subsequent work by Dutcher and colleagues placed it on linkage group XI, near the PF2 locus but capable of recombining with it.
Huang B, Piperno G, Ramanis Z, Luck DJ (1981) Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function. J Cell Biol 88:80-88
Holmes JA, Johnson DE, Dutcher SK (1993) Linkage group XIX of Chlamydomonas reinhardtii has a linear map. Genetics 133:865-874
CC-1925 sun1 mt+
$30.00
$30.00
From David Luck, Rockefeller University, July 1985
The sun1 mutant has no independent phenotype, but it suppresses the phenotype of the uniflagellate mutant uni1, causing an increase in the frequency of biflagellate cells in a culture.
Huang B, Ramanis Z, Dutcher SK, Luck DJ (1982) Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29:745-753
Dutcher SK (1986) Genetic properties of linkage group XIX in Chlamydomonas reinhardtii. Basic Life Sci 40:303-325
Ramanis Z, Luck DJ (1986) Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 83:423-426
Hall JL, Ramanis Z, Luck DJ (1989) Basal body/centriolar DNA: molecular genetic studies in Chlamydomonas. Cell 59:121-132
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