Strains

From David Luck, Rockefeller University, July 1985

Phenotype: uniflagellate

To the best of our knowledge, this is the same as CC-2506 uni1-1, the original uni1 mutant, which was induced by ICR-191 mutagenesis in the 137c background by Huang and colleagues in Luck’s laboratory. Most cells in a culture of this mutant are uniflagellate.

Huang B, Ramanis Z, Dutcher SK, Luck DJ (1982) Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29:745-753

Piasecki BP, Silflow CD (2009) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378

• Locus:
• UNI1
• Chromosome:
• 17

Chlamydomonas Genetics Center, Duke University, 1985

This is a subclone of CC-124, selected for having green cells in the dark (i.e. the wild type allele at the Y1 locus). We maintain it in the dark to preserve the phenotype.

Chlamydomonas Genetics Center, Duke University, 1985

This is a subclone of CC-125, selected for having green cells in the dark (i.e. the wild type allele at the Y1 locus). We maintain it in the dark to preserve the phenotype.

Chlamydomonas Genetics Center, Duke University, 1985

This strain is suppressed; see CC-1068 for a substitute.

• Locus:
• ARG7
• Chromosome:
• 1

Geraldine Fleming, Boynton-Gillham laboratory, Duke University, December 1985

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming in CC-125 and found to be an allele of the original act2 (see CC-1590). Stevens et al. identified this mutation as P54L (CCC>CTC)

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

Stevens DR, Atteia A, Franzén LG, Purton S (2001) Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii. Mol Gen Genet 264:790-795

• Locus:
• ACT2 [RPL36a]
• Chromosome:
• 6

Geraldine Fleming, Boynton-Gillham laboratory, Duke University, December 1985

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming in CC-125 and found to be an allele of the original act2 (see CC-1590). Stevens et al. identified this mutation as P54L (CCC>CTC).

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

Stevens DR, Atteia A, Franzén LG, Purton S (2001) Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii. Mol Gen Genet 264:790-795

• Locus:
• ACT2 [RPL36a]
• Chromosome:
• 6

Geraldine Fleming, Boynton-Gillham laboratory, Duke University, December 1985

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming in CC-125 and found to be an allele of the original act2 (see CC-1590). Stevens et al. identified this mutation as P54S (CCC>TCC).

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

Stevens DR, Atteia A, Franzén LG, Purton S (2001) Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii. Mol Gen Genet 264:790-795

• Locus:
• ACT2 [RPL36a]
• Chromosome:
• 6

Geraldine Fleming, Boynton-Gillham laboratory, Duke University, December 1985

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming in CC-125 and found to be an allele of the original act2 (see CC-1590). Stevens et al. identified this mutation as P54S (CCC>TCC).

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

Stevens DR, Atteia A, Franzén LG, Purton S (2001) Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii. Mol Gen Genet 264:790-795

• Locus:
• ACT2 [RPL36a]
• Chromosome:
• 6

From Alexander Chunayev, Leningrad State University, December 1985, replaced May 1989

Phenotype: carotenoid and chlorophyll b deficient

This is a yellowish green, light-sensitive strain. The cbn1 mutation by itself produces deficiency in chlorophyll b, but does not prevent phototrophic growth. However, lts1-135 impairs carotenoid synthesis and causes light sensitivity.

For more information on cbn1, please see CC-1354, and for more on mutants at the LTS1 locus, please see CC-4109.

• Locus:
• CBN1 [CAO1], LTS1 [PSY1]
• Chromosome:
• 1,11

From P.A. Lefebvre, University of Minnesota, March 1986

S1 C5 and S1 D2 (CC-2290) are very similar wild Chlamy isolates from the same soil sample, collected in Minnesota. They have extensive sequence polymorphisms with the lab strain of C. reinhardtii, but are fully interfertile with it. Crosses between the lab strain and these isolates have been used as the foundation of the molecular map of the genome. A cDNA library has been prepared from CC-2290, and additional genomic sequencing is being done. This strain can grow on nitrate.

Gross CH, Ranum LP, Lefebvre PA (1988) Extensive restriction fragment length polymorphisms in a new isolate of Chlamydomonas reinhardtii. Curr Genet 13:503-508

N.W. Gillham, Duke University, May 1986

Phenotype: abnormal cell division; antibiotic resistant (spectinomycin)

This is Gillham’s original mat3-2 isolate, designated M3-5-2-5. Originally thought to have modified transmission of the chloroplast genome, the mat3 mutants have been shown by Umen and Goodenough to have lesions in a retinoblastoma protein homolog involved in cell division. The altered plastid gene transmission is a secondary consequence of abnormally small cell size.

This mutation may become suppressed, and outcrossing to wild type may be necessary to recover the original phenotype.

Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Mating type linked mutations which disrupt the uniparental transmission of chloroplast genes in chlamydomonas. Genetics 115:677-684

Armbrust EV, Ibrahim A, Goodenough UW (1995) A mating type-linked mutation that disrupts the uniparental inheritance of chloroplast DNA also disrupts cell-size control in Chlamydomonas. Mol Biol Cell 6:1807-1818

Umen JG, Goodenough UW (2001) Control of cell division by a retinoblastoma protein homolog in Chlamydomonas. Genes Dev 15:1652-1661

• Locus:
• MAT3, rrnS
• Chromosome:
• 6,chloroplast

From Rene Matagne, University of Liege, May 1986

Mutants at the ARG4 locus are deficient in ornithine carbamoyltransferase. However, this strain currently does not show a clean arginine requirement and needs to be outcrossed to recover the original mutation if possible. This is the original arg4 mutant, UV-induced in Levine’s lab.

Hastings PJ, Levine EE, Cosbey E, Hudock MO, Gillham NW, Surzycki SJ, Loppes R, Levine RP (1965) The linkage groups of Chlamydomonas reinhardi. Microb Genet Bull 23:17-19

Loppes R (1969) A new class of arginine-requiring mutants in Chlamydomonas reinhardi. Mol Gen Genet 104:172-177

Loppes R (1970) Growth inhibition by NH 4 + ions in arginine-requiring mutants of Chlamydomonas reinhardi. Mol Gen Genet 109:233-240

Strijkert PJ, Loppes R, Sussenbach JS (1973) The actual biochemical block in the arg-2 mutant of Chlamydomonas reinhardi. Biochem Genet 8:239-248

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii.
Arch Microbiol 143:348-352

• Locus:
• ARG4 [OTC1]
• Chromosome:
• 12

From Rene Matagne, University of Liege, May 1986

Phenotype: requires arginine (suppressed)

Mutants at the ARG4 locus are deficient in ornithine carbamoyltransferase. However, this strain currently does not show a clean arginine requirement and needs to be outcrossed to recover the original mutation if possible. This is the original arg4 mutant, UV-induced in Levine’s lab. Please see CC-1997 for more information.

• Locus:
• ARG4 [OTC1]
• Chromosome:
• 12

From Rene Matagne, University of Liege, May 1986

Phenotype: requires arginine

This mutant was induced using nitrosoguanidine, by Loppes and Heindricks. It is a different allele from the original arg4, which was UV-induced by Levine (see CC-1997).

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii.
Arch Microbiol 143:348-352

• Locus:
• ARG4 [OTC1]
• Chromosome:
• 12

From Rene Matagne, University of Liege, May 1986

Phenotype: requires arginine

The arg8 mutants are deficient in argininosuccinate synthetase. They require arginine, and cannot grow on citrulline or ornithine. Loppes and Heindricks obtained three arg8 alleles, but this is the only one currently in the Chlamydomonas Center collection.

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii
Arch. Microbiol 143:348-352

From Rene Matagne, University of Liege, May 1986

Phenotype: requires arginine (suppressed)

The arg9 mutants are deficient in acetylornithine aminotransferase and require arginine, citrulline or ornithine for growth. The ARG9 locus was mapped to a position about 20 cM centromere-proximal to the mating type locus by Ferris and Goodenough.

This strain has acquired a suppressor. For a cleaner arg9 strain, see CC-2958.

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii. Arch Microbiol 143:348-352

Ferris PJ, Goodenough UW (1994) The mating-type locus of Chlamydomonas reinhardtii contains highly rearranged DNA sequences. Cell 76:1135-1145

• Locus:
• ARG9
• Chromosome:
• 6

From Rene Matagne, University of Liege, May 1986

Phenotype: requires arginine (suppressed)

The original arg10 mutant, induced using ethyl methanesulfonate by Loppes and collaborators, was deficient in acetylornithine glutamate transacetylase, and required arginine, citrulline or ornithine for growth. Unfortunately CC-2002, which is our only arg10 strain, has acquired a suppressor and is no longer auxotrophic. We are trying to recover the original mutant.

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii. Arch Microbiol 143:348-352

From Kwen-Sheng Chiang, University of Chicago, June 1986

Phenotype: antibiotic resistant (erythromycin, streptomycin)

This is a spontaneous diploid recovered in a mt+ strain.

Eves EM, Chiang KS (1982) Genetics of Chlamydomonas reinhardtii diploids. I. Isolation and characterization and meiotic segregation pattern of a homozygous diploid. Genetics 100:35-60

From George Witman, Worcester Foundation for Experimental Biology, to Elizabeth Harris, September 1986; converted to the public collection in 1998

Phenotype: impaired motility

The io- phenotype first appeared in a pf23 culture in Witman’s laboratory. Whereas pf23 has short paralyzed flagella and lacks inner dynein arms, the IO strain has paralyzed flagella of normal length and lacks the outer arm and most of the inner arms as well.

Culture Centre of Algae and Protozoa (CCAP), October 1986

Phenotype: abnormal cell division

This is the original cyt1 mutant, isolated by Warr in the 137c background. Its phenotype is aberrant cytokinesis, producing multinucleate and multiflagellate cells.

Warr JR (1968) A Mutant of Chlamydomonas reinhardii with abnormal cell division. J Gen Microbiol 52:243–251

Warr JR, Durber S (1971) Studies on the expression of a mutant with abnormal cell division in Chlamydomonas reinhardi. Exp Cell Res 64:463-469

Warr JR, Quinn D (1977) Low molecular weight sulphydryl compounds and the expression of a cell division mutant of Chlamydomonas reinhardi. Exp Cell Res 104:442-445

Ehler LL, Dutcher SK (1998) Pharmacological and genetic evidence for a role of rootlet and phycoplast microtubules in the positioning and assembly of cleavage furrows in Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 40:193-207

• Locus:
• CYT1
• Chromosome:
• 6

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen and Ebersold after mutagenesis with ICR-170. It was tentatively mapped to linkage group VI, but not placed precisely on that linkage group. It shows reduced recombination frequency in crosses, and appears to be deficient in recombination repair.

Rosen H, Ebersold WT (1972) Recombination in relation to ultraviolet sensitivity in Chlamydomonas reinhardi. Genetics 71, 247-253

Portney MD, Rosen H (1980) The effect of caffeine on repair in Chlamydomonas reinhardtii. II. Interaction of repair systems. Mutat Res 70:311-321

Rosen H, Rehn MM, Johnson BA (1980) T he effect of caffeine on repair in Chlamydomonas reinhardtii. I. Enhancement of recombination repair. Mutat Res 70:301-309

Vlček D, Podstavkova S, Miadoková E, Vlcková A (1991) The repair systems in green algae as compared with present knowledge in heterotrophic microorganisms. Arch Protistenkd. 139:193-199

From Howard Rosen, California State University, Los Angeles, October 1986

This is a UV-sensitive mutant isolated by Rosen after mutagenesis with ICR-170.

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen after mutagenesis with ICR-170. CC-2382 is the same mutation in mt-.

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen and Ebersold after UV mutagenesis. Cells of this mutant show increased UV survival when treated with caffeine, an indication that they are not deficient in recombination repair.

Rosen H, Ebersold WT (1972) Recombination in relation to ultraviolet sensitivity in Chlamydomonas reinhardi. Genetics 71:247-253

Portney MD, Rosen H (1980) The effect of caffeine on repair in Chlamydomonas reinhardtii. II. Interaction of repair systems. Mutat Res 70:311-321

Rosen H, Rehn MM, Johnson BA (1980) The effect of caffeine on repair in Chlamydomonas reinhardtii. I. Enhancement of recombination repair. Mutat Res 70:301-309

Vlček D, Podstavkova S, Miadoková E, Vlcková A (1991) The repair systems in green algae as compared with present knowledge in heterotrophic microorganisms. Arch Protistenkd. 139:193-199

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen and Ebersold after UV mutagenesis. Cells of this mutant are deficient in recombination repair, but in contrast to uvsE1, this deficiency is not associated with meiotic recombination.

Rosen H, Ebersold WT (1972) Recombination in relation to ultraviolet sensitivity in Chlamydomonas reinhardi. Genetics 71:247-253

Portney MD, Rosen H (1980) The effect of caffeine on repair in Chlamydomonas reinhardtii. II. Interaction of repair systems. Mutat Res 70:311-321

Vlček D, Podstavkova S, Miadoková E, Vlcková A (1991) The repair systems in green algae as compared with present knowledge in heterotrophic microorganisms. Arch Protistenkd. 139:193-199

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen and Ebersold after UV mutagenesis. Cells of this mutant are deficient in recombination repair, but in contrast to uvsE1, this deficiency is not associated with meiotic recombination. See CC-2383 for the same mutation in mt+.

Rosen H, Ebersold WT (1972) Recombination in relation to ultraviolet sensitivity in Chlamydomonas reinhardi. Genetics 71:247-253

Portney MD, Rosen H (1980) The effect of caffeine on repair in Chlamydomonas reinhardtii. II. Interaction of repair systems. Mutat Res 70:311-321

Vlček D, Podstavkova S, Miadoková E, Vlcková A (1991) The repair systems in green algae as compared with present knowledge in heterotrophic microorganisms. Arch Protistenkd 139:193-199

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen after UV mutagenesis. See CC-2384 for the same mutation in mt-.

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen after UV mutagenesis.

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen after UV mutagenesis. See CC-2385 for the same mutation in mt-.

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This is a UV-sensitive mutant isolated by Rosen after UV mutagenesis.