Strains

From Howard Rosen, California State University, Los Angeles, October 1986

Phenotype: UV sensitive

This strain carries both the uvsE4 and uvsE5 mutations. Please see CC-2021 and CC-2022 for additional information.

From Catherine Nicholson-Guthrie, Indiana University School of Medicine, November 1986

Phenotype: yellow in the dark

Please see CC-2911 for more information on the y-y mutant. The rev mutation partially reverses the y-y phenotype, leading to chlorophyll accumulation greater than that of y-y but less than that of y-1.

Nicholson-Guthrie CS (1983) Chlorophyll inheritance in Chlamydomonas. Masking of Y-1 gene by Y-Y gene. J Hered 74:16-18

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • psbA
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • psbA
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: requires acetate

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569

  • Locus:
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: herbicide resistant (atrazine, bromacil)

This mutation in the psbA gene confers resistance to atrazine and bromacil. It was obtained by Mets and Galloway by mutagenesis of strain 2137 (see CC-3269) with 5-fluorodeoxyuridine plus ethyl methanesulfonate. The mutation alters amino acid 256 of the D1 protein from Gly to Asp.

Galloway RE, Mets LJ (1984) Atrazine, bromacil, and diuron resistance in chlamydomonas: a single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol 74:469-474

Govindjee, Eggenberg P, Pfister K, Strasser RJ (1992) Chlorophyll a fluorescence decay in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii and the formate effect. Biochim Biophys Acta 1101:353-358

  • Locus:
  • psbA
  • Chromosome:
  • chloroplast

From Laurens Mets, University of Chicago, November 1986

Phenotype: herbicide resistant (atrazine, bromacil)

This mutation in the psbA gene confers resistance to atrazine and bromacil. It was obtained by Mets and Galloway by mutagenesis of strain 2137 (see CC-3269)with 5-fluorodeoxyuridine plus ethyl methanesulfonate. The mutation alters amino acid 256 of the D1 protein from Gly to Asp.

Galloway RE, Mets LJ (1984) Atrazine, bromacil, and diuron resistance in chlamydomonas: a single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol 74:469-474

Govindjee, Eggenberg P, Pfister K, Strasser RJ (1992) Chlorophyll a fluorescence decay in herbicide-resistant D1 mutants of Chlamydomonas reinhardtii and the formate effect. Biochim Biophys Acta 1101:353-358

  • Locus:
  • psbA
  • Chromosome:
  • chloroplast

Chlamydomonas Genetics Center, Duke University, 1986

Phenotype: cold sensitive on minimal medium

From CC-125 x CC-40 cr1 mt- (no longer in the collection)

The cr1 mutant in its original form is deficient in chloroplast ribosomes but accumulates 54S ribosomal subunits. It is a leaky acetate-requirer and is cold sensitive. With time, nearly all cr1 cultures acquire second-site mutations that reduce the accumulation of ribosomal subunit material. The ac20 mutation (see CC-43) is the best characterized of these; we don’t know if all the secondary mutations are ac20 alleles or if some are at different loci. It’s been a long time since this particular strain was tested for 54S accumulation.

Please see CC-41 for more information on cr1.

  • Locus:
  • CR1
  • Chromosome:
  • 12

From Ursula Goodenough, Washington University, December 1986, originally from Matsuda

Phenotype: does not mate

The fus mutants are blocked in adhesion of mating structures prior to sexual fusion. This is a spontaneous allele at the FUS1 locus, isolated in Sager’s 21 gr strain by Matsuda and colleague

Also see CC-1158 for information on the imp1 allele at the same locus.

Matsuda Y, Tamaki S, Tsubo Y (1978) Mating type specific induction of cell wall lytic factor by agglutination of gametes in Chlamydomonas reinhardtii. Plant Cell Physiol 19:1253-1261

Matsuda Y, Tamaki S, Tsubo Y (1981) The isolation and properties of the lytic enzyme of the cell wall released by mating gametes of Chlamydomonas reinhardtii. Plant Cell Physiol 22:1607-1611

Saito T, Matsuda Y (1984) Sexual agglutinin of mating-type minus gametes in Chlamydomonas reinhardii. I. Loss and recovery of agglutinability of gametes treated with EDTA. Exp Cell Res 152:322-330

Forest CL (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 88:613-621

Ferris PJ, Woessner JP, Goodenough UW (1996) A sex recognition glycoprotein is encoded by the plus mating-type gene fus1 of Chlamydomonas reinhardtii. Mol Biol Cell 7:1235-1248

Wang Q, Snell WJ (2003) Flagellar adhesion between mating type plus and mating type minus gametes activates a flagellar protein-tyrosine kinase during fertilization in Chlamydomonas. J Biol Chem 278:32936-32942

  • Locus:
  • FUS1
  • Chromosome:
  • 6

From Nicole Morel, Tufts University, January 1987

Phenotype: abnormal eyespot, altered phototaxis

Originally ey550, this is now known to be an allele at the EYE1 locus. Please see CC-1101 for more information on this locus.

  • Locus:
  • EYE1
  • Chromosome:
  • 10

From Nicole Morel, Tufts University, January 1987

Phenotype: abnormal eyespot, altered phototaxis

Originally ey627, this is now known to be an allele at the EYE1 locus. Please see CC-1101 for more information on this locus.

  • Locus:
  • EYE1
  • Chromosome:
  • 10

From Geraldine Fleming, Boynton-Gillham laboratory, Duke University, January 1987

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming and found to be an allele of the original act2 (see CC-1590). It shows an alteration in electrophoretic mobility of ribosomal protein “cyL38″, now known to be RPL36a.

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

  • Locus:
  • ACT2 [RPL36a]
  • Chromosome:
  • 6

From Geraldine Fleming, Boynton-Gillham laboratory, Duke University, January 1987

This is a mutant isolated by Fleming and found to be an allele of the original act2. It shows an alteration in electrophoretic mobility of ribosomal protein cyL38, now known to be RPL36a. Unlike act2, it is sensitive to cycloheximide.

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

From Geraldine Fleming, Boynton-Gillham laboratory, Duke University, January 1987

Phenotype: antibiotic resistant (cycloheximide)

This is a mutant isolated by Fleming and found to be an allele of the original act2 (see CC-1590). Protein “cyL38″ (RPL36a) has wild-type electrophoretic mobility in this strain.

Fleming GH, Boynton JE, Gillham NW (1987) The cytoplasmic ribosomes of Chlamydomonas reinhardtii: characterization of antibiotic sensitivity and cycloheximide-resistant mutants. Mol Gen Genet 210:419-428

  • Locus:
  • ACT2 [RPL36a]
  • Chromosome:
  • 6

Originally from George Hudock, this stock from Carolina Biological, March 1987

Phenotype: yellow in the dark

y2 is now thought to be an allele at the Y1 locus. Please see CC-735 for more information on this locus.

  • Locus:
  • Y1
  • Chromosome:
  • 15

Originally from George Hudock, this stock from Carolina Biological, March 1987

Phenotype: yellow in the dark

y2 is now thought to be an allele at the Y1 locus. Please see CC-735 for more information on this locus.

  • Locus:
  • Y1
  • Chromosome:
  • 15

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University..

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.

From N.E. Tolbert, Michigan State University, June 1987

Phenotype: inhibitor resistant (fluoroacetate)

This is a fluoroacetate resistant mutant isolated by Barbara Wilson in Tolbert’s laboratory, and described in her Ph.D. dissertation, Michigan State University.