Strains

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From P.A. Lefebvre, University of Minnesota, June 1988

Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA1 locus corresponds to the gene encoding the 62.5 kDa subunit of the dynein outer arm docking complex.

Kamiya R and Okamoto M (1985) A mutant of Chlamydomonas reinhardtii that lacks the flagellar outer dynein arm but can swim. J Cell Sci 74:181-191

Okagaki T and Kamiya R (1986) Cyclical bending of two outer-doublet microtubules in frayed axonemes of Chlamydomonas. Cell Motil Cytoskel 6:580-585

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Sakakibara H and Kamiya R (1989) Functional Recombination of Outer Dynein Arms with Outer Arm-Missing Flagellar Axonemes of a Chlamydomonas Mutant. J Cell Sci 92:77-83

Takada S, Sakakibara H, Kamiya R (1992) Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer-arm-missing axonemes. J Biochem 111:758-762

Takada S, Wilkerson CG, Wakabayashi K, Kamiya R, Witman GB (2002) The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly. Mol Biol Cell 13:1015-1029

  • Locus:
  • ODA1 [DCC2]
  • Chromosome:
  • 16

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2228 for more information on oda1.

Kamiya R and Okamoto M (1985) A mutant of Chlamydomonas reinhardtii that lacks the flagellar outer dynein arm but can swim. J Cell Sci 74:181-191

Okagaki T and Kamiya R (1986) Cyclical bending of two outer-doublet microtubules in frayed axonemes of Chlamydomonas. Cell Motil Cytoskel 6:580-585

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Sakakibara H and Kamiya R (1989) Functional Recombination of Outer Dynein Arms with Outer Arm-Missing Flagellar Axonemes of a Chlamydomonas Mutant. J Cell Sci 92:77-83

Takada S, Sakakibara H, Kamiya R (1992) Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer-arm-missing axonemes. J Biochem 111:758-762

Takada S, Wilkerson CG, Wakabayashi K, Kamiya R, Witman GB (2002) The outer dynein arm-docking complex: composition and characterization of a subunit (oda1) necessary for outer arm assembly. Mol Biol Cell 13:1015-1029

  • Locus:
  • ODA1 [DCC2]
  • Chromosome:
  • 16

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA2 locus corresponds to the gene encoding the outer arm dynein gamma heavy chain. The pf28 and sup-pf2 mutants are also alleles at this locus.

Brokaw CJ and Kamiya R (1987) Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function. Cell Motil Cytoskel 8:68-75

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Takada S, Kamiya R (1994) Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor. J Cell Biol 126:737-745

Takada S, Kamiya R (1997) Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules. Cell Motil Cytoskeleton 36:68-75

Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R (2008) Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryotic Cell 7:1136-1145

  • Locus:
  • ODA2 [DHC15]
  • Chromosome:
  • 11

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2230 for more information on oda2.

Brokaw CJ, Kamiya R (1987) Bending patterns of Chlamydomonas flagella: IV. Mutants with defects in inner and outer dynein arms indicate differences in dynein arm function. Cell Motil Cytoskel 8:68-75

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Takada S, Kamiya R (1994) Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor. J Cell Biol 126:737-745

Takada S, Kamiya R (1997) Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules. Cell Motil Cytoskeleton 36:68-75

Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R (2008) Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryotic Cell 7:1136-1145

  • Locus:
  • ODA2 [DHC15]
  • Chromosome:
  • 11

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA3 locus corresponds to the gene encoding an 83.4 kDa component of the outer dynein arm docking complex. This is the original oda3 mutant, UV-induced by Kamiya in the 137c background. It lacks outer dynein arms, swims more slowly than wild-type cells and lacks the backward swimming response to stimulation with intense light.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Koutoulis A, Pazour GJ, Wilkerson CG, Inaba K, Sheng H, Takada S, Witman GB (1997) The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex. J Cell Biol 137:1069-1080

  • Locus:
  • ODA3 [DCC1]
  • Chromosome:
  • 17

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2232 for more information on oda3.

Koutoulis A, Pazour GJ, Wilkerson CG, Inaba K, Sheng H, Takada S, Witman GB (1997) The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex. J Cell Biol 137:1069-1080

  • Locus:
  • ODA3 [DCC1]
  • Chromosome:
  • 17

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA4 locus was first identified by the sup-pf1 mutant (see CC-1397). The gene product has been identified as the beta heavy chain of the outer dynein arm.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Takada S and Kamiya R (1994) Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor. J Cell Biol 126:737-745

Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Hayashibe K, Shingyoji C, Kamiya R (1997) Induction of temporary beating in paralyzed flagella of Chlamydomonas mutants by application of external force. Cell Motil Cytoskeleton 37:232-239

Takada S and Kamiya R (1997) Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules. Cell Motil Cytoskeleton 36:68-75

Sakakibara H, Nakayama H (1998) Translocation of microtubules caused by the alphabeta, beta and gamma outer arm dynein subparticles of Chlamydomonas. J Cell Sci 111:1155-1164

  • Locus:
  • ODA4 [DHC14]
  • Chromosome:
  • 9

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2234 for the same mutation in mt+, and CC-1397 for more information on the ODA4 locus.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Takada S and Kamiya R (1994) Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor. J Cell Biol 126:737-745

Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Hayashibe K, Shingyoji C, Kamiya R (1997) Induction of temporary beating in paralyzed flagella of Chlamydomonas mutants by application of external force. Cell Motil Cytoskeleton 37:232-239

Takada S and Kamiya R (1997) Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules. Cell Motil Cytoskeleton 36:68-75

Sakakibara H and Nakayama H (1998) Translocation of microtubules caused by the alphabeta, beta and gamma outer arm dynein subparticles of Chlamydomonas. J Cell Sci 111:1155-1164

  • Locus:
  • ODA4 [DHC14]
  • Chromosome:
  • 9

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

This mutant was UV-induced in strain 137c by Kamiya. It swims, but more slowly than wild type, and it lacks the backward swimming response to stimulation with intense light.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995a) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Takada S, Kamiya R (1994) Functional reconstitution of Chlamydomonas outer dynein arms from alpha-beta and gamma subunits: requirement of a third factor. J Cell Biol 126:737-745

Takada S, Kamiya R (1997) Beat frequency difference between the two flagella of Chlamydomonas depends on the attachment site of outer dynein arms on the outer-doublet microtubules. Cell Motil Cytoskeleton 36:68-75

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB (2004) Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell 15:2729-2741

  • Locus:
  • ODA5 [DCC3]
  • Chromosome:
  • 7

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

For more information on oda5, please see CC-2236.

  • Locus:
  • ODA5 [DCC3]
  • Chromosome:
  • 7

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA6 locus corresponds to the gene encoding the dynein outer arm 70 kDa intermediate chain.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

Mitchell DR, Kang Y (1991) Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene. J Cell Biol 113:835-842

DiBella LM, Gorbatyuk O, Sakato M, Wakabayashi K, Patel-King RS, Pazour GJ, Witman GB, King SM (2005) Differential light chain assembly influences outer arm dynein motor function. Mol Biol Cell 16:5661-5674

  • Locus:
  • ODA6 [DIC2]
  • Chromosome:
  • 12

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2238 for more information on the ODA6 locus.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

Mitchell DR, Kang Y (1991) Identification of oda6 as a Chlamydomonas dynein mutant by rescue with the wild-type gene. J Cell Biol 113:835-842

DiBella LM, Gorbatyuk O, Sakato M, Wakabayashi K, Patel-King RS, Pazour GJ, Witman GB, King SM (2005) Differential light chain assembly influences outer arm dynein motor function. Mol Biol Cell 16:5661-5674

  • Locus:
  • ODA6 [DIC2]
  • Chromosome:
  • 12

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The oda7 mutation was UV-induced by Kamiya in the 137c background. The mutant lacks outer dynein arms. It swims, but more slowly than wild type, and lacks the backward swimming response to stimulation with intense light. Freshour et al. showed that the ODA7 protein interacts with both outer row and I1 inner row dyneins.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

Freshour J, Yokoyama R, Mitchell DR (2007) Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins. J Biol Chem 282:5404-5412

  • Locus:
  • ODA7 [DAU1]
  • Chromosome:
  • 1

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

This mutant was UV-induced in strain 137c by Kamiya. It swims, but more slowly than wild type, and it lacks the backward swimming response to stimulation with intense light.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

  • Locus:
  • ODA8 [DLU2]
  • Chromosome:
  • 1

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

For more information on oda8, please see CC-2242.

  • Locus:
  • ODA8 [DLU2]
  • Chromosome:
  • 1

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

The ODA9 locus corresponds to the gene for the 78 kDa dynein outer arm intermediate chain.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

  • Locus:
  • ODA9 [DIC1]
  • Chromosome:
  • 12

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

Please see CC-2244 for more information on oda9.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

  • Locus:
  • ODA9 [DIC1]
  • Chromosome:
  • 12

From Ritsu Kamiya, Nagoya University, May 1988

This strain is questionable. CC-2247 oda10 mt- should be substituted.

  • Locus:
  • ODA10
  • Chromosome:
  • 8

From Ritsu Kamiya, Nagoya University, May 1988

Phenotype: impaired motility

This mutant was UV-induced in strain 137c by Kamiya. It swims, but more slowly than wild type, and it lacks the backward swimming response to stimulation with intense light.

Kamiya R (1988) Mutations at twelve independent loci result in absence of outer dynein arms in Chlamydomonas reinhardtii. J Cell Biol 107:2253-2258

Kamiya R (1995) Exploring the function of inner and outer dynein arms with Chlamydomonas mutants. Cell Motil Cytoskel 32:98-102

  • Locus:
  • ODA10
  • Chromosome:
  • 8

From Rene Matagne, University of Liege, May 1988

Phenotype: dies in the dark

This is a terminal deletion mutation in mitochondrial DNA that eliminates the cob (cytochrome b) gene. It is an obligate photoautotroph, i.e. cannot grow on acetate medium in the dark. DNA analysis shows both monomer and dimer forms of the genome, and a heterogeneous size distribution.

CC-2654 is a subclone of this strain.

Matagne RF, Michel-Wolwertz MR, Munaut C, Duyckaerts C, Sluse F (1989) Induction and characterization of mitochondrial DNA mutants in Chlamydomonas reinhardtii. J Cell Biol 108:1221-1226

Randolph-Anderson BL, Boynton JE, Gillham NW, Harris EH, Johnson AM, Dorthu MP, Matagne RF (1993) Further characterization of the respiratory deficient dum-1 mutation of Chlamydomonas reinhardtii and its use as a recipient for mitochondrial transformation. Mol Gen Genet 236:235-244

  • Locus:
  • cob
  • Chromosome:
  • mitochondrial