Strains

From Robert Spreitzer, University of Nebraska, June 1988

Phenotype: requires acetate

Please see CC-1227 for more information on the ac214 mutation.

  • Locus:
  • AC214 [PRK1]
  • Chromosome:
  • 12

From Robert Spreitzer, University of Nebraska, June 1988

Phenotype: requires acetate

To the best of our knowledge, this is the same as CC-2056.

  • Locus:
  • Chromosome:
  • chloroplast

From Robert Spreitzer, University of Nebraska, June 1988

Phenotype: requires acetate

To the best of our knowledge, this is the same as CC-2058.

  • Locus:
  • Chromosome:
  • chloroplast

From Howard Rosen, California State University, Los Angeles, August 1988

Phenotype: UV sensitive; requires p-aminobenzoic acid and thiamine; inhibitor resistant (methionine sulfoximine)

The uvsE9 mutation was UV-induced by Rosen. CC-2385 carries the same mutation in mt-.

This strain was reported to carry the msr1, pab2, and thi3 mutations. It should be grown on medium containing yeast extract.

  • Locus:
  • MSR1, PAB2, THI3
  • Chromosome:
  • 1

Boynton-Gillham laboratory, Duke University, 1988

Phenotype: antibiotic and herbicide resistant (DCMU, spectinomycin)

The dr-u-2 mutation confers resistance to DCMU (please see CC-1402 for more information). spr-u-1-6-2 is a spectinomycin resistance mutation (see CC-110).

  • Locus:
  • psbA, rrnS
  • Chromosome:
  • chloroplast

From P.A. Lefebvre, University of Minnesota, August 1988

Phenotype: impaired motility

This is an allele at the LF2 locus, isolated in the Lefebvre lab after mutagenesis of strain 137c with methyl methanesulfonate, and characterized by Barsel et al.

Please see CC-803 for more information on the LF2 locus.

Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648

  • Locus:
  • LF2
  • Chromosome:
  • 12

From P.A. Lefebvre, University of Minnesota, August 1988

Phenotype: impaired motility

This is an allele at the LF2 locus, isolated in the Lefebvre lab after mutagenesis with methyl methanesulfonate, and characterized by Barsel et al. Please see CC-803 for more information on the LF2 locus.

*This strain has reverted and is no longer recommended.

Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648

  • Locus:
  • LF2
  • Chromosome:
  • 12

From P.A. Lefebvre, University of Minnesota, August 1988

Phenotype: impaired motility

When grown without aeration, cultures of lf3-2 strains show about 30-80% cells with long flagella and less than 15% with stumpy flagella.

This mutant was isolated by Lefebvre and colleagues in a derivative of strain 137C, ‘NO’ mt+, also known as ‘R3 NM’ mt+ (CC-620); this mutant was originally designated WH9.

Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648

Tam LW, Dentler WL, Lefebvre PA (2003) Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas. J Cell Biol 163:597-607

  • Locus:
  • LF3
  • Chromosome:
  • 1

From P.A. Lefebvre, University of Minnesota, August 1988

This strain, S1 D2, and S1 C5 (CC-1952) are very similar wild Chlamy isolates from Minnesota. They have extensive sequence polymorphisms with the lab strain of C. reinhardtii, but are fully interfertile with it. Crosses between the lab strain and these isolates have been used as the foundation of the molecular map A cDNA library has been prepared from CC-2290. This strain can grow on nitrate.

CC-2290 is the strain needed to use the molecular mapping kit developed in David Stern’s laboratory.

Gross CH, Ranum LP, Lefebvre PA (1988) Extensive restriction fragment length polymorphisms in a new isolate of Chlamydomonas reinhardtii. Curr Genet 13:503-508

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

This is a field isolate from Pennsylvania. Phylogenetic comparisons of this strain and other C. reinhardtii isolates have been made by Liss, Kirk, Beyser and Fabry, using introns in the Actin and Ypt 4 genes. See GenBank sequences U55905, U55906, and U70569. This strain can grow on nitrate.

Spanier JG, Graham JE, Jarvik JW (1992) Isolation and preliminary characterization of three Chlamydomonas strains interfertile with Chlamydomonas reinhardtii (Chlorophyta). J Phycol 28:822-828

Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

This is a field isolate from Florida. Phylogenetic comparisons of this strain and other C. reinhardtii isolates have been made by Liss, Kirk, Beyser and Fabry, using introns in the Actin and Ypt 4 genes. See GenBank sequences U55889, U55890, and U70561. This strain can grow on nitrate.

Note: a very old typographical error was discovered in April 2008. This strain is actually #224, not #124.

Spanier JG, Graham JE, Jarvik JW (1992) Isolation and preliminary characterization of three Chlamydomonas strains interfertile with Chlamydomonas reinhardtii (Chlorophyta). J Phycol 28:822-828

Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

This is a field isolate from Pennsylvania. Phylogenetic comparisons of this strain and other C. reinhardtii isolates have been made by Liss, Kirk, Beyser and Fabry, using introns in the Actin and Ypt 4 genes. See GenBank sequences U55891, U55892, and U70562. This strain can grow on nitrate.

Spanier JG, Graham JE, Jarvik JW (1992) Isolation and preliminary characterization of three Chlamydomonas strains interfertile with Chlamydomonas reinhardtii (Chlorophyta). J Phycol 28:822-828

Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

Phenotype: short flagella; probably requires thiamine

Three minor axonemal polypeptides are missing in two-dimensional gels of flagellar proteins. In contrast to other short-flagella mutants (e.g., McVittie’s), shf1 mutants are normal in ultrastructure and swimming does occur.

Borisy GG, Cleveland DB, Murphy DB (1984) Approaches to flagellar assembly and size control using stumpy- and short-flagella mutants of Chlamydomonas reinhardtii. Mol Biol Cytoskel 245-255

Kuchka MR, Jarvik JW (1987) Short-Flagella Mutants of Chlamydomonas reinhardtii. Genetics 115:685-691

  • Locus:
  • SHF1
  • Chromosome:
  • 6

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

Phenotype: impaired motility, short flagella

In combination with certain shf1 alleles, the shf2 mutations produce cells lacking flagella altogether.

Borisy GG, Cleveland DB, Murphy DB (1984) Approaches to flagellar assembly and size control using stumpy- and short-flagella mutants of Chlamydomonas reinhardtii. Mol Biol Cytoskel 245-255

Kuchka MR, Jarvik JW (1987) Short-Flagella Mutants of Chlamydomonas reinhardtii. Genetics 115:685-691

  • Locus:
  • SHF2
  • Chromosome:
  • 1

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

Phenotype: short flagella

Three minor axonemal polypeptides are missing in two-dimensional gels of flagellar proteins. In contrast to other short-flagella mutants (e.g., McVittie’s), shf1 mutants are normal in ultrastructure and swimming does occur.

Borisy GG, Cleveland DB, Murphy DB (1984) Approaches to flagellar assembly and size control using stumpy- and short-flagella mutants of Chlamydomonas reinhardtii. Mol Biol Cytoskel 245-255

Kuchka MR, Jarvik JW (1987) Short-Flagella Mutants of Chlamydomonas reinhardtii. Genetics 115:685-691

  • Locus:
  • SHF1
  • Chromosome:
  • 6

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

Phenotype: short flagella

Three minor axonemal polypeptides are missing in two-dimensional gels of flagellar proteins. In contrast to other short-flagella mutants (e.g., McVittie’s), shf1 mutants are normal in ultrastructure and swimming does occur.

Borisy GG, Cleveland DB, Murphy DB (1984) Approaches to flagellar assembly and size control using stumpy- and short-flagella mutants of Chlamydomonas reinhardtii. Mol Biol Cytoskel 245-255

Kuchka MR, Jarvik JW (1987) Short-Flagella Mutants of Chlamydomonas reinhardtii. Genetics 115:685-691

  • Locus:
  • SHF1
  • Chromosome:
  • 6

From Jonathan Jarvik, Carnegie-Mellon University, February 1989

Phenotype: short flagella

This mutant was UV-induced by Jarvik and colleagues.

Borisy GG, Cleveland DB, Murphy DB (1984) Approaches to flagellar assembly and size control using stumpy- and short-flagella mutants of Chlamydomonas reinhardtii. Mol Biol Cytoskel 245-255

Kuchka MR, Jarvik JW (1987) Short-Flagella Mutants of Chlamydomonas reinhardtii. Genetics 115:685-691

From Niki Robertson, Boynton-Gillham laboratory, Duke University, 1989

Phenotype: requires acetate

This strain is the product of a cross of CC-1290 FUD18 mt+ to CC-1291 FUD18 mt- (no longer in the collection). However, we are now distributing CC-4150 as the representative FUD18 strain, since it has been studied more recently by Girard-Bascou and colleagues.

  • Locus:
  • atpF
  • Chromosome:
  • chloroplast

From Pierre Bennoun, Institut de Biologie Physico-Chimique, Paris, April 1989

Phenotype: antibiotic resistant (chloramphenicol)

Bennoun P, Delepelaire P, Delosme M (1981) A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol. Curr Genet 3:251-253

From Pierre Bennoun, Institut de Biologie Physico-Chimique, April 1989

Phenotype: antibiotic resistant (chloramphenicol)

This mutant is resistant to chloramphenicol. Sequencing in the Boynton-Gillham laboratory in 1989 showed that this mutation is a change in the RIB1 region of the 23S chloroplast ribosomal RNA.

Bennoun P, Delepelaire P, Delosme M (1981) A uniparental mutant of Chlamydomonas reinhardtii resistant to chloramphenicol. Curr Genet 3:251-253

From Marilyn Kobayashi, University of California Berkeley, Niyogi lab, March 2013

Phenotype: chlorophyll deficient

This is a carotenoid-deficient, light sensitive mutant.

Chemerilova VI (1978) Study of pigmentation-modifying mutations in Chlamydomonas reinhardi strains of different ploidy. II. Compounds forlts1 mutations and their use for obtaining triploid cultures. Genetika 14:154-162

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

  • Locus:
  • LTS1
  • Chromosome:
  • 11

From David Luck, Rockefeller University, May 1989

This flagellar suppressor mutant was described by Piperno and colleagues.

Piperno G, Mead K, LeDizet M, Moscatelli A (1994) Mutations in the "dynein regulatory complex" alter the ATP-insensitive binding sites for inner arm dyneins in Chlamydomonas axonemes. J Cell Biol 125:1109-1117

Piperno G (1995) Regulation of dynein activity within Chlamydomonas flagella. Cell Motil Cytoskel 32:103-105

From David Luck, Rockefeller University, May 1989

For more information on pf15, please see CC-1033, and for sup-pf1, see CC-1397.

  • Locus:
  • PF15 [KAT2], SUP-PF1 [DHC14]
  • Chromosome:
  • 3,9

From David Luck, Rockefeller University, May 1989

This strain was received as pf-17 sup-3, with citations to the following references:

For additional information on PF17, please see CC-1035; for sup-pf3, please see CC-1399.

Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-124

Brokaw CJ, Luck DJ, Huang B (1982) Analysis of the movement of Chlamydomonas flagella: the function of the radial-spoke system is revealed by comparison of wild-type and mutant flagella. J Cell Biol 92:722-732

  • Locus:
  • PF17 [RSP9], SUP-PF3 [PF2]
  • Chromosome:
  • 7,11

From David Luck, Rockefeller University, May 1989

For additional information on these mutations, please see CC-1035 pf17 and CC-1397 sup-pf1.

  • Locus:
  • PF17 [RSP9], SUP-PF1 [DHC14]
  • Chromosome:
  • 7,9

From David Luck, Rockefeller University, May 1989

For more information on the mutant alleles in this strain please see CC-1033 pf15, CC-1397 sup-pf1, and CC-2506 uni1.

  • Locus:
  • PF15 [KAT2], SUP-PF1 [DHC14], UNI1
  • Chromosome:
  • 3,9,17

From David Luck, Rockefeller University, May 1989

For more information on the mutant alleles in this strain please see CC-1035 pf17, CC-1397 sup-pf1, and CC-2506 uni1.

  • Locus:
  • PF17 [RSP9], SUP-PF1 [DHC14], UNI1
  • Chromosome:
  • 7,9,17

From David Luck, Rockefeller University, May 1989

For more information on the mutant alleles in this strain please see CC-1035 pf17, CC-1397 sup-pf1, CC-2376 mbo1, and CC-2506 uni1.

  • Locus:
  • MBO1, PF17 [RSP9], SUP-PF1 [DHC14], UNI1
  • Chromosome:
  • 6,7,9,17

From David Luck, Rockefeller University, May 1989

For more information on the mutant alleles in this strain please see CC-1033 pf15, CC-1397 sup-pf1, and CC-2376 mbo1.

  • Locus:
  • MBO1, PF15 [KAT2], SUP-PF1 [DHC14]
  • Chromosome:
  • 3,6,9

From David Luck, Rockefeller University, May 1989

For more information on the mutant alleles in this strain please CC-1033 pf15, CC-1397 sup-pf1, CC-2376 mbo1 and CC-2506 uni1.

  • Locus:
  • MBO1, PF15 [KAT2], SUP-PF1 [DHC14], UNI1
  • Chromosome:
  • 3,6,9,17