Strains
CC-2508 uni1-3 mt+
$30.00
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: uniflagellate
This mutant was induced by ICR-191 mutagenesis in the 137c background by Huang and colleagues in Luck’s laboratory. Huang et al. called it uni4, but showed that it was allelic with uni1.
Huang B, Ramanis Z, Dutcher SK, Luck DJ (1982) Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29:745-753
CC-2509 uni1-4 mt-
$30.00
$30.00
From Gianni Piperno, Rockefeller University, June 1990
This mutant was induced by ICR-191 mutagenesis in the 137c background by Huang and colleagues in Luck’s laboratory. Huang et al. called it uni4, but showed that it was allelic with uni1.
Huang B, Ramanis Z, Dutcher SK, Luck DJ (1982) Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29:745-753
CC-2510 pf16B mt+
$30.00
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: impaired motility
This mutant, an allele of the original pf16 (see CC-1034), was isolated by Huang et al. after mutagenesis with nitrosoguanidine.
Huang B, Piperno G, Luck DJ (1979) Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms. J Biol Chem 254:3091-3099
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
CC-2511 pf16BR3 mt+
$30.00
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: impaired motility at high temperature
This is a temperature sensitive intragenic revertant of pf16B (CC-2510).
It swims normally at 21 degrees C but is paralyzed at 32 degrees.
Dutcher SK, Huang B, Luck DJ (1984) Genetic dissection of the central pair microtubules of the flagella of Chlamydomonas reinhardtii. J Cell Biol 98:229-236
Porter ME, Power J, Dutcher SK (1992) Extragenic suppressors of paralyzed flagellar mutations in Chlamydomonas reinhardtii identify loci that alter the inner dynein arms. J Cell Biol 118:1163-1176
CC-2512 apm1A mt+
$30.00
$30.00
From Gianni Piperno, Rockefeller University, June 1990
Phenotype: herbicide resistant (amiprophos methyl, oryzalin)
Mutants at the APM1 locus are resistant to the microtubule-inhibiting herbicides amiprophos methyl and oryzalin. This allele, apm1A, was isolated by Hall et al. in the 137c background. See also CC-3004 for an additional allele isolated by James et al.
James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics 118:141-147
Hall JL, Ramanis Z, Luck DJ (1989) Basal body/centriolar DNA: molecular genetic studies in Chlamydomonas. Cell 59:121-132
CC-2513 FUD39 mt+
$30.00
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, August 1990
Phenotype: requires acetate
FUD39 is a mutation in the PSBP gene encoding protein OEE2. Despite isolation following 5-fluorodeoxyuridine treatment, which is generally considered to act preferentially on chloroplast DNA, this is a nuclear mutation.
CC-4142 and CC-4143 are more recently acquired stocks of this mutant.
de Vitry C, Olive J, Drapier D, Recouvreur M, Wollman FA (1989) Posttranslational events leading to the assembly of photosystem II protein complex: a study using photosynthesis mutants from Chlamydomonas reinhardtii. J Cell Biol 109:991-1006
Rova M, Franzén LG, Fredriksson PO, Styring S (1994) Photosystem II in a mutant of Chlamydomonas reinhardtii lacking the 23 kDa psbP protein shows increased sensitivity to photoinhibition in the absence of chloride. Photosynth Res 39:75-83
Rova EM, Mc Ewen B, Fredriksson PO, Styring S (1996) Photoactivation and photoinhibition are competing in a mutant of Chlamydomonas reinhardtii lacking the 23-kDa extrinsic subunit of photosystem II. J Biol Chem 271:28918-28924
CC-2514 BF25 mt+
$30.00
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, August 1990
Phenotype: requires acetate
BF25 is a mutation in the PSBP gene, encoding protein OEE2. CC-2890 is another culture of the same mutant.
de Vitry C, Olive J, Drapier D, Recouvreur M, Wollman FA (1989) Posttranslational events leading to the assembly of photosystem II protein complex: a study using photosynthesis mutants from Chlamydomonas reinhardtii. J Cell Biol 109:991-1006
Mayfield SP, Bennoun P, Rochaix JD (1987) Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photosystem II particles in Chlamydomonas reinhardtii. EMBO J 6:313-318
Mayfield SP, Rahire M, Frank G, Zuber H, Rochaix JD (1987) Expression of the nuclear gene encoding oxygen-evolving enhancer protein 2 is required for high levels of photosynthetic oxygen evolution in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 84:749-753
CC-2516 HN31 mt+
$30.00
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, August 1990
Phenotype: requires acetate
This is a nuclear mutant that is blocked in maturation of the chloroplast psaA mRNA.
Goldschmidt-Clermont M, Girard-Bascou J, Choquet Y, Rochaix JD (1990) Trans-splicing mutants of Chlamydomonas reinhardtii. Mol Gen Genet 223:417-425
CC-2519 FUD47 (psbD) mt+
$30.00
$30.00
From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, August 1990
Phenotype: requires acetate
The FUD47 mutation is a duplication that produces a frameshift in the chloroplast psbD gene.
Erickson JM, Rahire M, Malnoë P, Girard-Bascou J, Pierre Y, Bennoun P, Rochaix JD (1986) Lack of the D2 protein in a Chlamydomonas reinhardtii psbD mutant affects photosystem II stability and D1 expression. EMBO J 5:1745-1754
From the Culture Centre of Algae and Protozoa (CCAP), August 1990
This is a mutant of C. moewusii isolated by Lewin, his strain RAL#7. The M.475 mutant was described as paralyzed, and slow to regenerate flagella after deflagellation. This mutant is also in the collection as CC-957, and CC-1482 and in combination with M.1051 as CC-1500.
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the Culture Centre of Algae and Protozoa (CCAP), August 1990
This is a motility mutant isolated by Lewin.
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
From the Culture Centre of Algae and Protozoa (CCAP), August 1990
This is a combination of Lewin’s mutants M.1001 and M.1017.
Lewin RA (1974) Genetic control of flagellar activity in Chlamydomonas moewusii. Phycologia 13:45-55
CC-2529 C. moewusii LM74
$30.00
$30.00
From the Culture Centre of Algae and Protozoa (CCAP), August 1990
This strain came originally from Lewin, but is not otherwise identified. The LM numbers were Lewin’s internal stock numbers, and not intended for publication, but CCAP had no other information on this strain.
CC-2530 vfl2 mt+
$30.00
$30.00
From Jonathan Jarvik, Carnegie-Mellon University, October 1990
Phenotype: variable number of flagella
This mutant has a variable number of flagella, usually zero to six. The flagella are of normal length, and the cells appear to be of normal size and shape. The mutation was UV-induced in strain ‘NO mt+’ (equivalent to CC-620) by Kuchka and Jarvik, originally vfl-2-220. Taillon et al. determined that vfl2 is a G to A transition mutation in the structural gene for centrin, producing a Glu to Lys change at position 101, the first amino acid of the E-helix of the protein’s third EF-hand.
Kuchka MR, Jarvik JW (1982) Analysis of flagellar size control using a mutant of Chlamydomonas reinhardtii with a variable number of flagella. J Cell Biol 92:170-175
Jarvik JW, Suhan JP (1991) The role of the flagellar transition region: inferences from the analysis of aChlamydomonas mutant with defective transition region structures. J Cell Sci 99:731-740
Taillon BE, Adler SA, Suhan JP, Jarvik JW (1992) Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas. J Cell Biol 119:1613-1624
Harper JDI (1999) Chlamydomonas Cell Cycle Mutants. Int Rev Cytol 189:131-176
CC-2531 vfl2 mt-
$30.00
$30.00
From Jonathan Jarvik, Carnegie-Mellon University, October 1990
Phenotype: variable number of flagella
The VFL2 locus has been shown to be the gene encoding centrin. For more information, please see CC-2530.
CC-2535 mud1 (cob1 F129L)
$30.00
$30.00
From Pierre Bennoun, Institut de Biologie Physico-Chimique, April 1989
Phenotype: antibiotic resistant (myxothiazol, mucidin)
This mutation is a Phe to Leu change at amino acid position 129 in the mitochondrial cytochrome b gene, conferring resistance to myxothiazol and mucidin.
Bennoun P, Delosme M, Kück U (1991) Mitochondrial genetics of Chlamydomonas reinhardtii: resistance mutations marking the cytochrome b gene. Genetics 127:335-343
Remacle C, Matagne RF (1993) Transmission, recombination and conversion of mitochondrial markers in relation to the mobility of a group I intron in Chlamydomonas. Curr Genet 23:518-525
From Ursula Goodenough, Washington University, November 1990
This is a mt+/mt+ fusion diploid.
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
From P.A. Lefebvre, University of Minnesota, December 1990
This is a tetrad product from a cross of CC-1373 C. smithii mt+ x CC-2645, used for molecular mapping as described by Ranum et al. for a similar set of progeny from CC-1373 x CC-29.
Ranum LP, Thompson MD, Schloss JA, Lefebvre PA, Silflow CD (1988) Mapping flagellar genes in Chlamydomonas using restriction fragment length polymorphisms. Genetics 120:109-122
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