Strains Archives - Page 45 of 131 - Chlamydomonas Resource Center

* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $1000.00. Subscriptions cannot be used for CLiP mutants, BACs nor fosmids.

CC-2686 st1-1 mt-

$30.00

From Steven Ball, Lille University of Science and Technology, March 1992, his strain 17

Phenotype: altered starch metabolism

This mutant, which was isolated in the 137c background, accumulates much less starch than wild type cells. ADP-glucose pyrophorphorylase is altered so that it is much less sensitive to activation by 3-phosphoglycerate.

Ball S, Marianne T, Dirick L, Fresnoy M, Delrue B, Decq A (1991) A Chlamydomonas reinhardtii low-starch mutant is defective for 3-phosphoglycerate activation and orthophosphate inhibition of ADP-glucose pyrophosphorylase. Planta 185:17-26

CC-2687 st2-1 mt-?

$30.00

From Steven Ball, Lille University of Science and Technology, March 1992, his strain 18B

Phenotype: altered starch metabolism

This mutant, which was isolated in the 137c background, is amylose-defective, and accumulates a modified amylopectin. The st2 mutants are analogous to waxy (amylose-deficient) endosperm mutants of higher plants.

Delrue B, Fontaine T, Routier F, Decq A, Wieruszeski JM, Van Den Koornhuyse N, Maddelein ML, Fournet B, Ball S (1992) Waxy Chlamydomonas reinhardtii: monocellular algal mutants defective in amylose biosynthesis and granule-bound starch synthase activity accumulate a structurally modified amylopectin. J Bacteriol 174:3612-3620

CC-2688 st2-2 mt-?

$30.00

From Steven Ball, Lille University of Science and Technology, March 1992, his strain 25B

Phenotype: altered starch metabolism

CC-2689 st2-2 pab2 ac14 mt+

$30.00

From Steven Ball, Lille University of Science and Technology, March 1992, his strain B5

Phenotype: altered starch metabolism; requires acetate and p-aminobenzoic acid

This strain is amylose-defective, and accumulates a modified amylopectin. The st2 mutants are analogous to waxy (amylose-deficient) endosperm mutants of higher plants. Because of the ac14 and pab2 mutations, this strain requires acetate and para-amino-benzoic acid (for which yeast extract will suffice).

Locus:
AC14, PAB2
Chromosome:
1

CC-2690 st2-1 pab2 ac14 mt+

$30.00

From Steven Ball, Lille University of Science and Technology, March 1992, his strain B20

Phenotype: altered starch metabolism; requires acetate and p-aminobenzoic acid

This strain is amylose-defective, and accumulates a modified amylopectin. The st2 mutants are analogous to waxy (amylose-deficient) endosperm mutants of higher plants. Because of the pab2 and ac14 mutations, this strain requires acetate and para-amino-benzoic acid (for which yeast extract will suffice).

Locus:
AC14, PAB2
Chromosome:
1

CC-2691 L-23 (pf18 derivative)

$30.00

From Robert Bloodgood, University of Virginia, April 1992

Phenotype: impaired motility

L-23 is a Chlamydomonas mutant obtained by MNNG mutagenesis of pf-18 followed by cell sorting. It has totally lost binding of a monoclonal antibody to an N-linked carbohydrate epitope on FMG-1B, exhibits normal binding of a monoclonal antibody to a protein epitope on FMG-1B and exhibits greatly increased binding of concanavalin A. This suggests a glycosylation mutant that results in a high mannose form (instead of the normal complex carbohydrate form) at the many glycosylation sites on FMG-1B (the major flagellar membrane glycoprotein) as well on other glycoproteins. Unpublished preliminary data from a Spanish carbohydrate lab suggest that this strain has a mutation in a mannosidase enzyme.

Bloodgood RA, Salomonsky NL, Reinhart FD (1987) Use of carbohydrate probes in conjunction with fluorescence-activated cell sorting to select mutant cell lines of Chlamydomonas with defects in cell surface glycoproteins. Exp Cell Res 173:572-585

Bloodgood RA, Salomonsky NL (1989) Use of a novel Chlamydomonas mutant to demonstrate that flagellar glycoprotein movements are necessary for the expression of gliding motility. Cell Motil Cytoskeleton 13:1-8

Locus:
PF18, FMG1
Chromosome:
2,9

CC-2692 Ds521 mt- [Mets LM3-4D1C]

$30.00

From Laurens Mets, University of Chicago, April 1992

Phenotype: requires acetate

This strain, designated LM3-4D1C, is a segregant from a tetrad carrying the Ds521 CAB deficiency mutation described originally in Galloway and Mets (1989). Ds521 was originally isolated by FuDR/EMS mutagenesis of the chloroplast DCMU-resistant mutant Dr2 with selection for DCMU sensitivity. Ds521 has reduced chlorophyll per cell and a high chlorophyll a/b ratio compared to wild type cells, with loss of a large fraction of the chl a/b light-harvesting complex.

Galloway R, Mets L (1989) Positive selection for sensitivity of Chlamydomonas reinhardtii to Photosystem II-inhibiting herbicides. Biochim Biophys Acta 975:66-71

CC-2693 sr-u-sm2 mt+ [Mets DES 15]

$30.00

From Laurens Mets, University of Chicago, April 1992

Phenotype: antibiotic resistant (streptomycin)

This strain was derived by backcrossing a mt+ strain carrying the sr-u-sm2 streptomycin resistance mutation to a 137c- strain originally obtained by Mets from Levine. It has been used as the parent strain for deriving many additional mutants.

Please see CC-215 for more information on the sr-u-sm2 mutation.

Locus:
rps12
Chromosome:
chloroplast

CC-2696 A4d (Ds521, psbA deletion) mt+

$30.00

From Laurens Mets, University of Chicago, April 1992

Phenotype: requires acetate

The A4d strain was derived from a cross between B1 (= CC-2694, which is no longer in the collection) and LM3-4D1c (= CC-2692). It carries the nuclear Ds521 CAB deficiency mutation and the B1 deletion mutation in the chloroplast psbA gene. It has been used extensively in studies of PSI by Mets and others.

Owens TG, Webb SP, Mets L, Alberte RS, Fleming GR (1989) Antenna structure and excitation dynamics in photosystem I. II. Studies with mutants of Chlamydomonas reinhardtii lacking photosystem II. Biophys J 56:95-106

Werst M, Jia Y, Mets L, Fleming GR (1992) Energy transfer and trapping in the photosystem I core antenna. A temperature study. Biophys J 61:868-878

Locus:
psbA
Chromosome:
chloroplast

CC-2698 cia2

$30.00

From James Moroney, Louisiana State University, April 1992

Phenotype: altered CO2 assimilation

This strain requires 5% CO2 for growth on minimal medium. The cia2 mutation affects the CAH3 carbonic anhydrase gene.

Moroney JV, Tolbert NE, Sears BB (1986) Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii. Mol Gen Genet 204:199-203

CC-2699 cia3

$30.00

From James Moroney, Louisiana State University, April 1992

Phenotype: altered CO2 assimilation

This strain requires 5% CO2 for growth on minimal medium. The cia3 mutation is in the CAH3 carbonic anhydrase gene, and is allelic to ca1 (CC-1219) and to cia4 (CC-2701).

Moroney JV, Tolbert NE, Sears BB (1986) Complementation analysis of the inorganic carbon concentrating mechanism of Chlamydomonas reinhardtii. Mol Gen Genet 204:199-203

Katzman GL, Carlson SJ, Marcus Y, Moroney JV, Togasaki RK (1994) Carbonic Anhydrase Activity in Isolated Chloroplasts of Wild-Type and High-CO2-Dependent Mutants of Chlamydomonas reinhardtii as Studied by a New Assay. Plant Physiol 105:1197-1202

**Van Hunnik E and Sültemeyer D (2002) A possible role for carbonic anhydrase in the lumen of chloroplast thylakoids in green algae. Funct. Plant Biol 29:243-249

Hanson DT, Franklin LA, Samuelsson G, Badger MR (2003) The Chlamydomonas reinhardtii cia3 mutant lacking a thylakoid lumen-localized carbonic anhydrase is limited by CO2 supply to rubisco and not photosystem II function in vivo. Plant Physiol 132:2267-2275

Duanmu D, Wang Y, Spalding MH (2009) Thylakoid lumen carbonic anhydrase (CAH3) mutation suppresses air-Dier phenotype of LCIB mutant in Chlamydomonas reinhardtii. Plant Physiol 149:929-937

Locus:
CAH3
Chromosome:
9

CC-2701 cia4

$30.00

From James Moroney, Louisiana State University, April 1992

Phenotype: altered CO2 assimilation

This strain requires 5% CO2 for growth on minimal medium. The cia4 mutation affects the CAH3 carbonic anhydrase gene, and is allelic to ca1 (CC-1219) and to cia3. Please see CC-2699 for more information on this locus.

Locus:
CAH3
Chromosome:
9

CC-2702 cia5 mt+

$30.00

From James Moroney, Louisiana State University, April 1992

Phenotype: altered CO2 assimilation

This strain requires 5% CO2 for growth on minimal medium. In contrast to other cia mutants, cia5 fails to synthesize any of the components of the CO2 concentrating system, including the periplasmic carbonic anhydrase, when switched to an environment low in CO2. This is a mutation in the CCM1 gene, which has been identified as the “master regulator” of the carbon concentrating mechanism in Chlamydomonas.

Moroney JV, Husic HD, Tolbert NE, Kitayama M, Manuel LJ, Togasaki RK (1989) Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO(2) Concentrating Mechanism. Plant Physiol 89:897-903

Marek LF, Spalding MH (1991) Changes in Photorespiratory Enzyme Activity in Response to Limiting CO(2) in Chlamydomonas reinhardtii. Plant Physiol 97:420-425

Spalding MH, Winder TL, Anderson JC, Geraghty AM, Marek LF (1991) Changes in protein and gene expression during induction of the CO2-concentrating mechanism in wild-type and mutant Chlamydomonas. Can J Bot 69:1008-1016

Xiang Y, Zhang J, Weeks DP (2001) The Cia5 gene controls formation of the carbon concentrating mechanism in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 98:5341-5346

Fukuzawa H, Miura K, Ishizaki K, Kucho KI, Saito T, Kohinata T, Ohyama K (2001) Ccm1, a regulatory gene controlling the induction of a carbon-concentrating mechanism in Chlamydomonas reinhardtii by sensing CO2 availability. Proc Natl Acad Sci U S A 98:5347-5352

Locus:
CIA5 [CCM1]
Chromosome:
2

CC-2703 er-u-1a cpDNA deletion mt+

$30.00

Boynton-Gillham laboratory, Duke University

Phenotype: antibiotic resistant (erythromycin)

Derived by Scott Newman in the Boynton-Gillham laboratory, 1992. This strain was obtained by transformation of CC-744, a psbA deletion mutant, with plasmid P-262, a construct with a deletion in the intergenic region between psbA and the gene encoding 23S rRNA.

Newman SM, Harris EH, Johnson AM, Boynton JE, Gillham NW (1992) Nonrandom distribution of chloroplast recombination events in Chlamydomonas reinhardtii: evidence for a hotspot and an adjacent cold region. Genetics 132:413-429

Locus:
rrnL
Chromosome:
chloroplast

CC-2704 cadA

$30.00

From Rene Matagne, University of Liege, April 1992

Phenotype: inhibitor resistant (cadmium)

The cadA and cadB mutations are unlinked. Both are partially dominant. The cadB mutation alone confers resistance intermediate between cadA and wild type.

Collard JM, Matagne RF (1990) Isolation and genetic analysis of Chlamydomonas reinhardtii strains resistant to cadmium. Appl Environ Microbiol 56:2051-2055

CC-2705 cadB

$30.00

From Rene Matagne, University of Liege, April 1992

Phenotype: inhibitor resistant (cadmium)

The cadA and cadB mutations are unlinked. Both are partially dominant. The cadB mutation alone confers resistance intermediate between cadA and wild type.

Collard JM, Matagne RF (1990) Isolation and genetic analysis of Chlamydomonas reinhardtii strains resistant to cadmium. Appl Environ Microbiol 56:2051-2055

CC-2706 cadA cadB

$30.00

From Rene Matagne, University of Liege, April 1992

Phenotype: inhibitor resistant (cadmium)

The cadA and cadB mutations are unlinked. Both are partially dominant. The cadB mutation alone confers resistance intermediate between cadA and wild type.

Collard JM, Matagne RF (1990) Isolation and genetic analysis of Chlamydomonas reinhardtii strains resistant to cadmium. Appl Environ Microbiol 56:2051-2055

CC-2753 Fl 39

$30.00

From A. Tremolieres, CNRS, Gif-sur-Yevette, May 1992

Phenotype: requires acetate

This is a non-photosynthetic mutant isolated by Garnier and colleagues. It is deficient in photosystem II, lacking some of the reaction center polypeptides, and also shows deficiency in cytochrome b-559.

Garnier J, Guyon D, Picaud A (1979) Characterization of new strains of non-photosynthetic mutants of Chlamydomonas reinhardtii. I: fluorescence, photochemical activities, chlorophyllprotein complexes. Plant Cell Physiol 20:1013-1027

Maroc J, Garnier J (1979) Characterization of new strains of nonphotosynthetic mutants of Chlamydomonas reinhardtii II. Quinones and cytochromes b-559, b-563 and c-553 in twelve mutants having impaired Photosystem II function. Plant Cell Physiol. 20:1029-1040

Garnier J, Maroc J (1983) Physiol. Vegetale 21:361-365

Garnier J, Maroc J (1984) Chloroplast Proteins Related to Photosystem II in Chlamydomonas Reinhardtii: Mutants and Trypsin-Treated Chloroplast Particles. Adv. Photosyn. Res. 3:183-186

Maroc J, Guyon D, Gamier J (1983) Characterization of new strains of nonphotosynthetic mutants of Chlamydomonas reinhardtii III. Photosystem II-related thylakoid proteins in five mutants and double mutants. Plant Cell Physiol 24:1217-1230

Seras M, Garnier J, Trémoli`res A, Guyon D (1989) Lipid biosynthesis in cells of the wild type and of two photosynthetic mutants of Chlamydomonas reinhardtii. Plant Physiol Biochem 27:393–399

CC-2754 Fl 39 Pg 28

$30.00

From A. Tremolieres, CNRS, Gif-sur-Yevette, May 1992

Phenotype: requires acetate

Fl-39 is a non-photosynthetic mutant originally isolated by Garnier and colleagues (please see CC-2753). Irraditaion of this strain produced the double mutant Fl 38 Pg28, which also lacks the photosynthetic reaction center protein CP 0.

CC-2756 Pg 27

$30.00

From A. Tremolieres, CNRS, Gif-sur-Yevette, May 1992

Phenotype: requires acetate; chlorophyll b deficient

This mutant lacks chlorophyll b and a chlorophyll-binding protein of the photosynthetic reaction center.

Garnier J, Maroc J (1984) Chloroplast Proteins Related to Photosystem II in Chlamydomonas Reinhardtii: Mutants and Trypsin-Treated Chloroplast Particles. Adv. Photosyn. Res. 3:183-186

CC-2768 y27 mt-

$30.00

From Alexander Chunayev, St. Petersburg State University, May 1992

Phenotype: chlorophyll deficient, yellow in the dark

This is a chlorophyll-deficient mutant, yellow when grown mixotrophically, also known as yel-27. The published literature is inconsistent on whether this mutation is in the nuclear or chloroplast genome.

Gyurján I, Yurina NP, Thurischeva MS, Odintsova MS (1979a) Altered chloroplast ribosomal proteins in a yellow mutant of Chlamydomonas reinhardii. Mol Gen Genet 170:203-211

Tugarinov et al. (1980) Sov Genet 16:1199-1206

Yurina et al. (1981) Sov Plant Physiol 28:117-121

Gyurjan et al. (1985) Photosynthetica 19:285-293

CC-2770 y76 mt-

$30.00

From Alexander Chunayev, St. Petersburg State University, May 1992

Phenotype: chlorophyll deficient, yellow in the dark

Also known as yel-76, this is a chlorophyll deficent mutant with a normal chl a/b ratio.

Gyurján I, Yurina NP, Turischeva MS, Odintsova MS, Alexandrova NN (1980) Protein deficient chloroplast ribosomes in a yellow mutant of Chlamydomonas reinhardii. Planta 147, 287-294

Yurina et al. (1981) Soviet Plant Physiol. 28:117-121

CC-2803 rbcL (disruption) sr-u-2-60 spr-u-1-6-2 mt+ [Newman 25B1]

$30.00

Scott Newman in Boynton-Gillham laboratory, Duke University, 1992

Phenotype: requires acetate; antibiotic resistant (spectinomycin, streptomycin)

This strain was obtained by co-transformation of CC-125 with P-183 (sr spr) and P-270, which is a BamHI-EcoRI fragment internal to the rbcL gene, with a Pst site replaced by insertion of a 0.48 kb fragment from the yeast plasmid YEp24.

Newman SM, Gillham NW, Harris EH, Johnson AM, Boynton JE (1991) Targeted disruption of chloroplast genes in Chlamydomonas reinhardtii. Mol Gen Genet 230:65-74

Locus:
rbcL, rrnS
Chromosome:
chloroplast