Strains

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: does not mate; requires arginine

Cells with the imp3 mutation agglutinate strongly with partners of opposite mating type, but the initial adhesion is lost, cell walls are not shed, and gamete fusion is rare. Saito et al. have shown this mutant to be defective in production of cAMP in response to flagellar adhesion.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Saito T, Small L, Goodenough UW (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat. J Cell Biol 122:137-147

• Locus:
• ARG7
• Chromosome:
• 1

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: does not mate; requires arginine

This is imp4a (see CC-2921) in an arg7 background.

• Locus:
• ARG7
• Chromosome:
• 1

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: does not mate; requires arginine

This is imp4a (see CC-2921) in an arg2 background.

• Locus:
• ARG7
• Chromosome:
• 1

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: requires acetate, somewhat chlorophyll deficient

This is a diploid construct containing ac29a and another ac29 mutation, newly isolated in 1995, plus the nic7 mutation and its wild type allele (nic7 ac29a mt-/ + ac29-x mt+). This strain is nic+ but shows the ac29 (yellow colony) phenotype.

• Locus:
• AC29 [ALB3]
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University February 1995

Phenotype: requires acetate, somewhat chlorophyll deficient

This is a diploid construct containing ac29a and another ac29 mutation, newly isolated in 1995, plus the nic7 mutation and its wild type allele (nic7 ac29a mt-/ + ac29-x mt+). This strain is nic+ but shows the ac29 (yellow colony) phenotype.

• Locus:
• AC29 [ALB3]
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: requires acetate, somewhat chlorophyll deficient

This is a diploid construct containing ac29a and another ac29 mutation, newly isolated in 1995, plus the nic7 mutation and its wild type allele (nic7 ac29a mt-/ + ac29-x mt+). This strain is nic+ but shows the ac29 (yellow colony) phenotype.

• Locus:
• AC29 [ALB3]
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University, February 1995

This is a diploid strain that is nic-, possibly containing a new nic7 mutation in the mt+ chromosome. Please see CC-85 for more information on the NIC7 locus.

• Locus:
• NIC7
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University, February 1995

This is an apparent mitotic recombinant between ac29a and mt, hence nic7 ac29a mt- / nic7 ac29a mt+, creating a novel mutant combination in the plus chromosome that could in theory be recovered by haploidizing.

• Locus:
• NIC7
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University, February 1995

Phenotype: requires nicotinamide

Product from a cross of CC-421 nic7 ac29a to a wild type NIT+ strain, by Carol Hwang in Ursula Goodenough’s laboratory. This strain can grow on nitrate.

• Locus:
• NIC7
• Chromosome:
• 6

From Patrick Ferris, Goodenough lab, Washington University, February 1995, as 432 prog.8.

Phenotype: requires acetate, somewhat chlorophyll deficient

Isolated by crossing pac32-1 (CC-3044) to CC-85 nic7 to obtain a haploid containing the ac29-3 deletion mutation induced in a diploid strain. Ferris has demonstrated that the mating type region in CC-3054 is haploid based on DNA analysis, and that the mutation can be transformed with the wild type AC29 gene.

The new ac29 mutation in this strain is as 3 kb deletion, now known as ac29-3. It is stable and more easily scored than other ac29 alleles.

See CC-44 for more information on the protein encoded by the AC29 gene, and reference citations.

• Locus:
• AC29 [ALB3]
• Chromosome:
• 6

From Keith Kozminski in Joel Rosenbaum’s laboratory, Yale University, April 1995

This strain expresses epitope-tagged alpha1-tubulin to a high and constant level. It was created by transformation in March 1991. nit1-305 cells were cotransformed with pMN24 (nitrate reductase) and a construct containing the Chlamydomonas alpha1-tubulin gene tagged with a nine amino acid epitope from influenza virus hemagglutinin (HA). To obtain a high level of expression, the alpha1-tubulin promoter was replaced with the Rubisco S2 promoter.

Kozminski KG, Diener DR, Rosenbaum JL (1993) High level expression of nonacetylatable alpha-tubulin in Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 25:158-170

From David Bollivar in Samual Beale’s laboratory, Brown University, April 1995

Phenotype: chlorophyll deficient, requires acetate; wall deficient

This is a derivative of a chlB disruption strain created in Mike Timko’s laboratory, crossed with CC-406 to create a cell wall deficient derivative. This strain was used for chloroplast isolation by Bollivar and Beale.

Bollivar DW, Beale SI (1995) Formation of the isocyclic ring of chlorophyll by isolated Chlamydomonas reinhardtii chloroplasts. Photosynth Res 43:113-124

• Locus:
• chlB
• Chromosome:
• chloroplast

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. This strain showed questionable mating with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. In Bell’s tests, this strain did not mate with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. Bell did not record whether this mated with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. Bell did not record whether this mated with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. In Bell’s tests, this strain did not mate with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at Farnham, Quebec, about 50 km southeast of Montreal. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. Bell reported that this strain appeared to be homothallic.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.