Strains

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. Bell did not record whether this mated with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. Bell was uncertain about whether this mated with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. In Bell’s tests, this strain appeared to be immotile. Mating was not recorded.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. It is interfertile with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. Bell was uncertain whether this strain mated with the laboratory strain of C. reinhardtii.

From Graham Bell, McGill University, 1995

This is a field isolate from farmland at McDonald College, on Montreal Island, Quebec. Bell did not record whether this mated with the laboratory strain of C. reinhardtii.

From Ritsu Kamiya, University of Tokyo, May 1995

Phenotype: impaired motility

Kato T, Kagami O, Yagi T, Kamiya R (1993) Isolation of two species of Chlamydomonas reinhardtii flagellar mutants, ida5 and ida6, that lack a newly identified heavy chain of the inner dynein arm. Cell Struct Funct 18:371-377

• Locus:
• IDA6
• Chromosome:
• 13

From Ritsu Kamiya, University of Tokyo, May 1995

Phenotype: impaired motility

Please see CC-3090 for more information on the IDA6 locus.

Kato T, Kagami O, Yagi T, Kamiya R (1993) Isolation of two species of Chlamydomonas reinhardtii flagellar mutants, ida5 and ida6, that lack a newly identified heavy chain of the inner dynein arm. Cell Struct Funct 18:371-377

• Locus:
• IDA6
• Chromosome:
• 13

From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, by way of Sabeeha Merchant, UCLA, May 1995

Phenotype: requires acetate

This is a nuclear mutation that produces deficiency in the cytochrome b6/f complex.

Xie Z, Culler D, Dreyfuss BW, Kuras R, Wollman FA, Girard-Bascou J, Merchant S (1998) Genetic analysis of chloroplast c-type cytochrome assembly in Chlamydomonas reinhardtii: One chloroplast locus and at least four nuclear loci are required for heme attachment. Genetics 148:681-692

From Jacqueline Girard-Bascou, Institut de Biologie Physico-Chimique, by way of Sabeeha Merchant, UCLA, May 1995

Phenotype: requires acetate

This is a nuclear mutation that produces deficiency in the cytochrome b6/f complex.

Xie Z, Culler D, Dreyfuss BW, Kuras R, Wollman FA, Girard-Bascou J, Merchant S (1998) Genetic analysis of chloroplast c-type cytochrome assembly in Chlamydomonas reinhardtii: One chloroplast locus and at least four nuclear loci are required for heme attachment. Genetics 148:681-692

From Robert Spreitzer, University of Nebraska, to Nick Gillham at Duke, June 1995

This is a mutation that suppresses the light sensitivity of the Rubisco mutant rcl-u-1-10-6C (CC-1815).

Spreitzer RJ, Ogren WL (1983) Nuclear Suppressors of the Photosensitivity Associated with Defective Photosynthesis in Chlamydomonas reinhardii. Plant Physiol 71:35-39

From Peter Heifetz in Boynton-Gillham laboratory, Duke University, 1995

Phenotype: can grow at very high light intensity

This strain, which grows well at very high light intensities, was originally described as “125 S-2 high light suppressor”. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second).

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Peter Heifetz in Boynton-Gillham laboratory, Duke University, 1995

Phenotype: can grow at very high light intensity

This strain, which grows well at very high light intensities, was originally described as “125 S-3 high light suppressor”. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second).

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Peter Heifetz in Boynton-Gillham laboratory, Duke University, 1995

Phenotype: can grow at very high light intensity

This strain, which grows well at very high light intensities, was originally described as “125 S-7 high light suppressor”. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second).

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Peter Heifetz in Boynton-Gillham laboratory, Duke University

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). To the best of our knowledge, this strain, which was listed by Heifetz as “125 S-9 high light suppressor,” is identical to CC-3724, which was deposited in the collection by Britta Förster in 1999. Please see that strain for additional information and reference citations.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Boynton-Gillham laboratory, Duke University

Phenotype: herbicide resistant

The “Herb” strains are a collection of mutants resistant to experimental herbicides that were being developed by Ciba-Geigy Corporation in the 1980s. In 1986-87 Anita Johnson in the Boynton-Gillham laboratory tested the effects of these herbicides on C. reinhardtii, and isolated mutants resistant to them. Six compounds were tested under coded numbers. At least two of them were eventually made available commercially, as “Amber” and “Beacon.” Both are sulfonylurea herbicides. The mutants were isolated in the CC-124 wild type background.

Elizabeth Harris, Chlamydomonas Genetics Center, Duke University

Harris garden isolate #3

Isolated from garden soil collected in Durham, North Carolina in May 1991 by Elizabeth Harris. This is isolate #3. CC-2931 and CC-2932 are other isolates from the same soil sample, and have been more thoroughly analyzed.

From Laurens Mets, University of Chicago, 1995

Obtained for comparison with CC-1021, an isolate of strain 2137 received previously. Strain 2137 was generated by a cross of Ruth Sager’s strain 21 gr to the 137c strain (equivalent to CC-124) used in the Boynton/Gillham laboratory, and is the background of many non-photosynthetic mutants.

Since this strain came directly from the Mets laboratory, whereas CC-1021 was obtained second-hand, we recommend CC-3269 as the preferred isolate of strain 2137.

Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569