Strains
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This is CC-373 (an atpB deletion mutant) transformed with a construct containing the wild-type E. coli recA gene downstream from Chlamydomonas atpB.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This is CC-373 (an atpB deletion mutant) transformed with a construct containing the E. coli recA gene with a 24-amino acid internal deletion, downstream from Chlamydomonas atpB.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This is CC-373 (an atpB deletion mutant) transformed with a construct containing the E. coli recA gene with a 42-amino acid N-terminal deletion, downstream from Chlamydomonas atpB.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
Phenotype: chlorophyll deficient
This strain was derived as follows:
CC-373, an atpB deletion mutant, was transformed with the wild-type Chlamydomonas atpB gene to produce CC-3452, which was used as a control for CC-3453, CC-3454, and CC-3455 containing the same atpB gene upstream of three versions of the E. coli recA gene. (These strains are no longer in the collection.)
CC-3452 was then crossed to CC-1930 arg2 mt-, and a wild-type progeny clone (i.e. not requiring arginine) was seleted, to yield CC-3456.
CC-3456 was then transformed with an aadA construct disrupting the chlL gene (Goldschmidt-Clermont), flanked by direct repeats to evaluate recombination frequencies in the chloroplast. CC-3460 is one of the transformants recovered.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
Phenotype: chlorophyll deficient
Derivation of this strain was parallel to that of CC-3460, beginning with CC-3455, which was crossed to CC-1930 to give CC-3459 mt-, which was then transformed with the chlL disruption as in CC-3460.
Cerutti H, Johnson AM, Boynton JE, Gillham NW (1995) Inhibition of chloroplast DNA recombination and repair by dominant negative mutants of Escherichia coli RecA. Mol Cell Biol 15:3003-3011
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This strain was derived by transformation of CC-124 wild type mt- with an RbcS2::aadA::RbcS2 construct (P-681) as described by Cerutti et al. (1997a). This strain was characterized as having an unstable spectinomycin resistance phenotype.
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145:97-110
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Plant Cell 9:925-945
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This strain was derived by transformation of CC-124 wild type mt- with an RbcS2::aadA::RbcS2 construct (P-679) as described by Cerutti et al. (1997a).
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145:97-110
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Plant Cell 9:925-945
Heriberto Cerutti in Boynton-Gillham laboratory, Duke University, August 1997
This strain was derived by transformation of CC-124 wild type mt- with an RbcS2::aadA::RbcS2 construct (P-681) as described by Cerutti et al. (1997a). CC-3475 was characterized as having a stable spectinomycin resistance phenotype. It was fast growing, with an estimated number of 4 copies of the transforming aadA construct.
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics 145:97-110
Cerutti H, Johnson AM, Gillham NW, Boynton JE (1997) Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas. Plant Cell 9:925-945
Chlamydomonas Genetics Center, Duke University, 1997
Phenotype: wall deficient
From a series of four backcrosses of CC-125 wild type mt+ to obtain a better cw15 mt- isolate, beginning with CC-406.
This cell wall deficient strain swims and mates better than its parent CC-406. However, all cw15 cultures typically contain a high proportion of non-flagellated cells.
From Robert Drager and David Stern, Cornell University, December 1997
This is a transformant of the atpB deletion mutant strain CC-373 with pDG2, which contains the E. coli uidA (GUS) gene flanked by the Chlamydomonas petD promoter and rbcL 3′ UTR.
Rott R, Drager RG, Stern DB, Schuster G (1996) The 3' untranslated regions of chloroplast genes in Chlamydomonas reinhardtii do not serve as efficient transcriptional terminators. Mol Gen Genet 252:676-683
From Robert Drager and David Stern, Cornell University, December 1997
This is a transformant of the atpB deletion mutant strain CC-373 with pDAAD , which contains the E. coli aadA) gene flanked by the Chlamydomonas petD promoter and rbcL 3′ UTR.
Rott R, Drager RG, Stern DB, Schuster G (1996) The 3' untranslated regions of chloroplast genes in Chlamydomonas reinhardtii do not serve as efficient transcriptional terminators. Mol Gen Genet 252:676-683
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997
This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).
Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379
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