Strains

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From Pushpa Kathir and Carolyn Silflow, University of Minnesota, December 1997

This is a random progeny clone selected from a cross between CC-1690 (Sager’s 21 gr strain) and CC-1952 (a field isolate from Minnesota).

Kathir P, LaVoie M, Brazelton WJ, Haas NA, Lefebvre PA, Silflow CD (2003) Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot Cell 2:362-379

From William Zerges, in Bruce Kohorn’s laboratory, Duke University, January 1998

Phenotype: requires acetate

FUD34 is a mutation in the 5′ flanking region of the psbC gene, and tbc3-rb1 is a nuclear mutation in the TBC3 gene, which is involved in regulation of translation of psbC mRNA. This is Zerges strain RB1.27.

de Vitry C, Olive J, Drapier D, Recouvreur M, Wollman FA (1989) Posttranslational events leading to the assembly of photosystem II protein complex: a study using photosynthesis mutants from Chlamydomonas reinhardtii. J Cell Biol 109:991-1006

Rochaix JD, Kuchka M, Mayfield S, Schirmer-Rahire M, Girard-Bascou J, Bennoun P (1989) Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii. EMBO J 8:1013-1021

Zerges W, Girard-Bascou J, Rochaix JD (1997) Translation of the chloroplast psbC mRNA is controlled by interactions between its 5' leader and the nuclear loci TBC1 and TBC3 in Chlamydomonas reinhardtii. Mol Cell Biol 17:3440-3448

Zerges W, Auchincloss AH, Rochaix JD (2003) Multiple translational control sequences in the 5' leader of the chloroplast psbC mRNA interact with nuclear gene products in Chlamydomonas reinhardtii. Genetics 163:895-904

• Locus:
• psbC
• Chromosome:
• chloroplast

From William Zerges, in Bruce Kohorn’s laboratory, Duke University, January 1998, originally from Jacqueline Girard-Bascou

Phenotype: requires acetate

FUD34 is a mutation in the 5′ flanking region of the psbC gene, and tbc3-rb1 is a nuclear mutation in the TBC3 gene, which is involved in regulation of translation of psbC mRNA. This is Zerges strain RB1.27.

de Vitry C, Olive J, Drapier D, Recouvreur M, Wollman FA (1989) Posttranslational events leading to the assembly of photosystem II protein complex: a study using photosynthesis mutants from Chlamydomonas reinhardtii. J Cell Biol 109:991-1006

Rochaix JD, Kuchka M, Mayfield S, Schirmer-Rahire M, Girard-Bascou J, Bennoun P (1989) Nuclear and chloroplast mutations affect the synthesis or stability of the chloroplast psbC gene product in Chlamydomonas reinhardtii. EMBO J 8:1013-1021

Zerges W, Girard-Bascou J, Rochaix JD (1997) Translation of the chloroplast psbC mRNA is controlled by interactions between its 5' leader and the nuclear loci TBC1 and TBC3 in Chlamydomonas reinhardtii. Mol Cell Biol 17:3440-3448

Zerges W, Auchincloss AH, Rochaix JD (2003) Multiple translational control sequences in the 5' leader of the chloroplast psbC mRNA interact with nuclear gene products in Chlamydomonas reinhardtii. Genetics 163:895-904

• Locus:
• psbC
• Chromosome:
• chloroplast

From Xhiyi Xie and Sabeeha Merchant, UCLA, January 1998, originally from Jacqueline Girard-Bascou

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded ccsA gene.

Xie Z, Culler D, Dreyfuss BW, Kuras R, Wollman FA, Girard-Bascou J, Merchant S (1998) Genetic analysis of chloroplast c-type cytochrome assembly in Chlamydomonas reinhardtii: One chloroplast locus and at least four nuclear loci are required for heme attachment. Genetics 148:681-692

• Locus:
• ccsA
• Chromosome:
• chloroplast

From Xhiyi Xie and Sabeeha Merchant, UCLA, January 1998, originally from Jacqueline Girard-Bascou

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded ccsA gene.

Xie Z, Culler D, Dreyfuss BW, Kuras R, Wollman FA, Girard-Bascou J, Merchant S (1998) Genetic analysis of chloroplast c-type cytochrome assembly in Chlamydomonas reinhardtii: One chloroplast locus and at least four nuclear loci are required for heme attachment. Genetics 148:681-692

• Locus:
• ccsA
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her 56A13-2A

This is a disruption of ORF56, a reading frame close to the chloroplast-encoded petG gene.

Berthold DA, Schmidt CL, Malkin R (1995) The deletion of petG in Chlamydomonas reinhardtii disrupts the cytochrome bf complex. J Biol Chem 270:29293-29298

• Locus:
• petG
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her GT-1

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petG gene. We are uncertain whether it corresponds to one of the disruption mutants described in the following paper:

Berthold DA, Schmidt CL, Malkin R (1995) The deletion of petG in Chlamydomonas reinhardtii disrupts the cytochrome bf complex. J Biol Chem 270:29293-29298

• Locus:
• petG
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her GT-2

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petG gene. We are uncertain whether it corresponds to one of the disruption mutants described in the following paper:

Berthold DA, Schmidt CL, Malkin R (1995) The deletion of petG in Chlamydomonas reinhardtii disrupts the cytochrome bf complex. J Biol Chem 270:29293-29298

• Locus:
• petG
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her GT-3

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petG gene. We are uncertain whether it corresponds to one of the disruption mutants described in the following paper:

Berthold DA, Schmidt CL, Malkin R (1995) The deletion of petG in Chlamydomonas reinhardtii disrupts the cytochrome bf complex. J Biol Chem 270:29293-29298

• Locus:
• petG
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her D1

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

• Locus:
• petD
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her D2

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

• Locus:
• petD
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her Q1

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

• Locus:
• petD
• Chromosome:
• chloroplast

From Deborah Berthold, University of Illinois, 1998, her Q2

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

• Locus:
• petD
• Chromosome:
• chloroplast