Strains

From Deborah Berthold, University of Illinois, 1998, her F1

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

  • Locus:
  • petD
  • Chromosome:
  • chloroplast

From Deborah Berthold, University of Illinois, 1998, her F2

Phenotype: requires acetate

This is a mutation in the chloroplast-encoded petD gene.

  • Locus:
  • petD
  • Chromosome:
  • chloroplast

From Deborah Berthold, University of Illinois, 1998

This is Berthold’s strain A1, used as a control for the petD mutants in the series CC-3648 – CC-3653.

  • Locus:
  • petD
  • Chromosome:
  • chloroplast

From Deborah Berthold, University of Illinois, 1998

This is Berthold strain A2, used as a control for the petD mutants in the series CC-3648 – CC-3653.

  • Locus:
  • petD
  • Chromosome:
  • chloroplast

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires p-aminobenzoic acid

From a cross of CC-125 x CC-645

The pab2 mutant requires p-aminobenzoic acid. Yeast extract will satisfy this requirement.

  • Locus:
  • PAB2
  • Chromosome:
  • 1

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires nicotinamide

From a cross of CC-125 x CC-11, June 1998

This strain should require nicotinamide but may be difficult to score on minimal medium. Yeast extract will satisfy the nicotinamide requirement.

  • Locus:
  • NIC2 [QPT1]
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires thiamine

From a cross of CC-125 x CC-25

This strain requires thiamine (or yeast extract) for growth. However, like most other thi mutants, it is difficult to score on minimal medium. Restreaking or plating at low dilution helps accentuate the phenotype.

  • Locus:
  • THI8
  • Chromosome:
  • 5

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires thiamine

From a cross of CC-125 x CC-26

This strain requires thiamine (or yeast extract) for growth. However, like most other thi mutants, it is difficult to score on minimal medium. Restreaking or plating at low dilution will be helpful.

  • Locus:
  • THI9
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-125 x CC-1383

Mutants at the PF23 locus have flagella that are shorter than normal, and lack inner dynein arms. Please see CC-2494 for more information on this locus.

  • Locus:
  • PF23
  • Chromosome:
  • 11

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-125 x CC-1877

The pf28 mutant was found to be an allele at the ODA2 locus, which corresponds to the gene for the dynein outer arm gamma heavy chain. For more information on this mutant, please see CC-1877.

  • Locus:
  • PF28 [DHC15]
  • Chromosome:
  • 11

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-125 x CC-2289

  • Locus:
  • LF3
  • Chromosome:
  • 1

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: short flagella

From a cross of CC-125 x CC-2347

  • Locus:
  • SHF1
  • Chromosome:
  • 6

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-125 x CC-2377

Segal RA, Huang B, Ramanis Z, Luck DJ (1984) Mutant strains of Chlamydomonas reinhardtii that move backwards only. J Cell Biol 98:2026-2034

Tam LW, Lefebvre PA (2002) The Chlamydomonas MBO2 locus encodes a conserved coiled-coil protein important for flagellar waveform conversion. Cell Motil Cytoskeleton 51:197-212

  • Locus:
  • MBO2
  • Chromosome:
  • 9

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-1033 x CC-124.

  • Locus:
  • PF15 [KAT2]
  • Chromosome:
  • 3

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-1037 x CC-124, June 1998

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-1384 x CC-124

  • Locus:
  • PF24 [RSP2]
  • Chromosome:
  • 10

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-1385 x CC-124

  • Locus:
  • PF25 [RSP11]
  • Chromosome:
  • 10

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: impaired motility

From a cross of CC-2378 x CC-124

From Sigrid Jacobshagen, Western Kentucky University, June 1998

This strain was derived by co-transformation of CC-125 with pCAB-ARS and 8.5 kb cablI-1/nitl, with selection for growth on nitrate followed by selection for constitutive expression of arylsulfatase. It was developed in the course of studies of circadian clock regulation of CAB II (LHCB) genes.

Jacobshagen S, Kindle KL, Johnson CH (1996) Transcription of CABII is regulated by the biological clock in Chlamydomonas reinhardtii. Plant Mol Biol 31:1173-1184

From Eli Greenbaum, Oak Ridge National Laboratory, July 1998

Phenotype: requires acetate

B4 is a mutant deficient in Photosystem I, isolated by treatment with 5-fluorodeoxyuridine followed by metronidazole selection as described by Roitgrund and Mets, 1990. It was characterized by Greenbaum et al. as a nuclear mutation that affects splicing of the chloroplast-encoded psaA gene.

Roitgrund C, Mets LJ (1990) Localization of two novel chloroplast genome functions: trans-splicing of RNA and protochlorophyllide reduction. Curr Genet 17:147-153

Greenbaum E, Lee JW, Tevault CV, Blankinship SL, Mets LJ (1995) CO2 fixation and photoevolution of H2 and O2 in a mutant of Chlamydomonas lacking Photosystem I. Nature 376:438-441

Lee JW, Tevault CV, Owens TG, Greenbaum E (1996) Oxygenic Photoautotrophic Growth Without Photosystem I. Science. 273:364-367

Greenbaum E, Lee JW, Tevault CV, Blankinship SL, Owens TG, Mets LJ (1997) Photosystem-I Measurements in Mutants B4 and F8 of Chlamydomonas. Science 277:166-167

Cournac L, Redding K, Bennoun P, Peltier G (1997) Limited photosynthetic electron flow but no CO2 fixation in Chlamydomonas mutants lacking photosystem I. FEBS Lett. 416:65-68

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: antibiotic resistant (anisomycin)

From a cross of CC-399 x CC-124

  • Locus:
  • PDR1
  • Chromosome:
  • 16

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires nicotinamide

From a cross of CC-599 x CC-124

This nicotinamide-requiring mutant maps to the sparsely populated linkage group XV. Like all nic mutants, it can be grown either with supplemental nicotinamide or with yeast extract. Please see CC-599 for additional information.

  • Locus:
  • NIC1
  • Chromosome:
  • 14

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires acetate

From a cross of CC-567 x CC-124

This mutant is acetate-requiring but its photosynthetic defect has never been determined.

  • Locus:
  • AC210
  • Chromosome:
  • 3

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires acetate

From a cross of CC-538 ac40 to wild type. Please see CC-539 for more information on the AC40 locus.

  • Locus:
  • AC40
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires acetate

From a cross of CC-544 to wild type

  • Locus:
  • AC80
  • Chromosome:
  • 2

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires acetate

From a cross of CC-548 to wild type. Please see CC-548 for more information on ac115.

  • Locus:
  • AC115
  • Chromosome:
  • 1,8

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires acetate

From a cross of CC-548 to wild type. Please see CC-548 for more information on ac115.

  • Locus:
  • AC115
  • Chromosome:
  • 1,8

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires arginine

For more information on the ARG7 locus see CC-48.

  • Locus:
  • ARG7
  • Chromosome:
  • 1

Chlamydomonas Genetics Center, Duke University, 1998

Phenotype: requires arginine

From a cross of CC-125 x CC-1861.

CC-1861 arg7 mt-, which is no longer in the collection, was itself a product of a cross in 1984 of CC-125 x CC-51, a suppressed arg7 strain. When created, CC-1861 was a clean, easily scored arginine-requiring strain, but it too eventually acquired a second-site suppressor. CC-3681 has been stable, but should be retested before use.

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Peter Luykx, University of Miami, January 1999

Phenotype: deficient in osmoregulation

This is an osmoregulatory mutant that grows best on TAP medium supplemented with .06 M sucrose, sorbitol or lactose. It was obtained in 1992 by UV mutagenesis of CC-124 wild type mt-, backcrossed and re-isolated five times since then. The strains used for backcrossing were CC-124, CC-125 and CC-1036.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole inChlamydomonas reinhardtii. Protoplasma 200:99-111