Strains

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the ADF1 locus, obtained by insertional mutagenesis with NIT1. For more information on this locus, please see CC-2919.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• ADF1
• Chromosome:
• 9

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the ADF1 locus, obtained by insertional mutagenesis with NIT1. For more information on this locus, please see CC-2919.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• ADF1
• Chromosome:
• 9

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the ADF1 locus, obtained by insertional mutagenesis with NIT1. For more information on this locus, please see CC-2919.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• ADF1
• Chromosome:
• 9

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the ADF1 locus, obtained by UV mutagenesis. For more information on this locus, please see CC-2919.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• ADF1
• Chromosome:
• 9

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the FA1 locus, obtained by insertional mutagenesis with ARG7. For more information on this locus, please see CC-1370.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• FA1
• Chromosome:
• 6

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the FA1 locus, obtained by insertional mutagenesis with NIT1. For more information on this locus, please see CC-1370.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• FA1
• Chromosome:
• 6

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

This is an allele at the FA1 locus, obtained by insertional mutagenesis with NIT1. For more information on this locus, please see CC-1370.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

• Locus:
• FA1
• Chromosome:
• 6

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

Like fa1 (see CC-1370), the fa2 mutants are defective in flagellar autotomy, and fail to sever outer microtuble doublets in response to calcium. The FA2 gene encodes a NIMA-related kinase.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

Mahjoub MR, Montpetit B, Zhao L, Finst RJ, Goh B, Kim AC, Quarmby LM (2002) The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J Cell Sci 115:1759-1768

Mahjoub MR, Qasim Rasi M, Quarmby LM (2004) A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol Biol Cell 15:5172-5186

• Locus:
• FA2
• Chromosome:
• 7

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

Like fa1 (see CC-1370), the fa2 mutants are defective in flagellar autotomy, and fail to sever outer microtuble doublets in response to calcium. The FA2 gene encodes a NIMA-related kinase.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

Mahjoub MR, Montpetit B, Zhao L, Finst RJ, Goh B, Kim AC, Quarmby LM (2002) The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J Cell Sci 115:1759-1768

Mahjoub MR, Qasim Rasi M, Quarmby LM (2004) A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol Biol Cell 15:5172-5186

• Locus:
• FA2
• Chromosome:
• 7

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

Like fa1 (see CC-1370), the fa2 mutants are defective in flagellar autotomy, and fail to sever outer microtuble doublets in response to calcium. The FA2 gene encodes a NIMA-related kinase.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

Mahjoub MR, Montpetit B, Zhao L, Finst RJ, Goh B, Kim AC, Quarmby LM (2002) The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J Cell Sci 115:1759-1768

Mahjoub MR, Qasim Rasi M, Quarmby LM (2004) A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol Biol Cell 15:5172-5186

• Locus:
• FA2
• Chromosome:
• 7

From Lynne Quarmby and Rip Finst, Emory University, November 1999

Phenotype: impaired flagellar autotomy

Like fa1 (see CC-1370), the fa2 mutants are defective in flagellar autotomy, and fail to sever outer microtuble doublets in response to calcium. The FA2 gene encodes a NIMA-related kinase.

Finst RJ, Kim PJ, Quarmby LM (1998) Genetics of the deflagellation pathway in Chlamydomonas. Genetics 149:927-936

Mahjoub MR, Montpetit B, Zhao L, Finst RJ, Goh B, Kim AC, Quarmby LM (2002) The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. J Cell Sci 115:1759-1768

Mahjoub MR, Qasim Rasi M, Quarmby LM (2004) A NIMA-related kinase, Fa2p, localizes to a novel site in the proximal cilia of Chlamydomonas and mouse kidney cells. Mol Biol Cell 15:5172-5186

• Locus:
• FA2
• Chromosome:
• 7

Anita Lardans, Boynton-Gillham laboratory, Duke University, 1999

Phenotype: requires acetate; herbicide resistant (atrazine, bromacil, metribuzin)

This strain was derived from a cross of CC-3382 “Leu suppressor mt+” (no longer in the collection) to CC-124 wild type mt-. The submission notes for CC-3766 describe it as psbA A251L. We believe that this is the same site-directed A251L mutation as is present in CC-3389, but that CC-3766 does not have the silent T247C mutation that is also in that strain. Please see CC-3389 for more information on this mutation.

Lardans A, Gillham NW, Boynton JE (1997) Site-directed mutations at residue 251 of the photosystem II D1 protein of Chlamydomonas that result in a nonphotosynthetic phenotype and impair D1 synthesis and accumulation. J Biol Chem 272:210-216

• Locus:
• psbA
• Chromosome:
• chloroplast

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived from transformation of CC-425 with plasmid pJD76, subsequently crossed with CC-2677 (nit1-305).

This strain does not express arylsulfatase activity when grown under sulfur-deficient conditions. As a group, the sac1 mutants fail to accumulate arylsulfatase or the proteins of the high-affinity sulfate transport system when transferred to sulfur-deficient medium. They are also unable to decrease photosynthesis under these conditions, with the result that they die when cultured in the light under sulfur-limiting conditions.

Davies JP, Yildiz F, Grossman AR (1994) Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell 6:53-63

Davies JP, Yildiz FH, Grossman A (1996) Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J 15:2150-2159

Moseley JL, Gonzalez-Ballester D, Pootakham W, Bailey S, Grossman AR (2009) Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics 181:889-905

• Locus:
• SAC1
• Chromosome:
• 3

From John Davies, Iowa State University, August 2000

This is the ars5-4 mutant (sac1::ARG7, see CC-3794) complemented with a SAC1 genomic clone.

Davies JP, Yildiz FH, Grossman A (1996) Sac1, a putative regulator that is critical for survival of Chlamydomonas reinhardtii during sulfur deprivation. EMBO J 15:2150-2159

• Locus:
• SAC1
• Chromosome:
• 3

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived from transformation of CC-425 with plasmid pJD76.

This strain shows low arylsulfatase activity when grown on sulfur-deficient medium.

Davies JP, Yildiz F, Grossman AR (1994) Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell 6:53-63

• Locus:
• SAC2
• Chromosome:

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67, described as “sac2-like.”

This strain shows constitutive expression of arylsulfatase under sulfur-replete conditions.

Davies JP, Yildiz F, Grossman AR (1994) Mutants of Chlamydomonas with Aberrant Responses to Sulfur Deprivation. Plant Cell 6:53-63

Davies JP, Yildiz FH, Grossman AR (1999) Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. Plant Cell 11:1179-1190

• Locus:
• NIT1
• Chromosome:
• 9

From John Davies, Iowa State University, August 2000

This is the ars10-6-6-2 mutant complemented with a cosmid containing the SAC3 gene.

Davies JP, Yildiz FH, Grossman AR (1999) Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. Plant Cell 11:1179-1190

• Locus:
• SNRK2.2 [SAC3]
• Chromosome:
• 12

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

The are12 mutant was derived by transformation of CC-425 with pJD67. This strain shows constitutive arylsulfatase activity on sulfur-replete medium.

Davies JP, Yildiz FH, Grossman AR (1999) Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. Plant Cell 11:1179-1190

• Locus:
• SNRK2.2 [SAC3]
• Chromosome:
• 12

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67.

Davies JP, Yildiz FH, Grossman AR (1999) Sac3, an Snf1-like serine/threonine kinase that positively and negatively regulates the responses of Chlamydomonas to sulfur limitation. Plant Cell 11:1179-1190

• Locus:
• SNRK2.2 [SAC3]
• Chromosome:
• 12

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67. The ars4-15 mutation is allelic with ars5-4 (see CC-3794).

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• SAC1
• Chromosome:
• 3

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67. The ars4-55 mutant is allelic with ars5-4 (see CC-3794).

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• SAC1
• Chromosome:
• 3

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67.

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• SAC4
• Chromosome:

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67.

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• SAC6
• Chromosome:

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67.

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• SAC7
• Chromosome:

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD67.

This strain does not express arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• ARS4
• Chromosome:
• 1

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD76, subsequently crossed to CC-2677.

This strain has low arylsulfatase activity when grow under sulfur-deficient conditions.

• Locus:
• NIT1
• Chromosome:
• 9

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD76.

This strain has low arylsulfatase activity when grow under sulfur-deficient conditions.

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD76.

This strain has low arylsulfatase activity when grow under sulfur-deficient conditions.

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD76.

This strain has low arylsulfatase activity when grow under sulfur-deficient conditions.

From John Davies, Iowa State University, August 2000

Phenotype: altered sulfur metabolism

Derived by transformation of CC-425 with pJD76.

This strain has low arylsulfatase activity when grow under sulfur-deficient conditions.