Strains

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T9B5) is in Class I.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T1A11) is in Class II.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T9D12) is in Class II.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T2H11) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T4C1) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T4D9) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T8B11) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T8D2) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T10A7) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T11A1) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T11E3) is in Class III.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T3A11) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T3C4) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T4A2) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T8C6) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T9B9) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T11C3) is in Class IV.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T1A12) is in Class V.

From Joel Rosenbaum, Yale University, January 2001

Phenotype: does not show gliding motility

Please see CC-3841 for more information on these non-gliding mutants.

This mutant (T2B1) is in Class V.

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

The fla15 mutant was isolated by Piperno in the 137c background, after mutagenesis with nitrosoguanidine. It shows temperature-conditional flagellar assembly, and is defective in retrograde intraflagellar transport. Iomini et al. found that the mutation is a missense mutation in a conserved cysteine in the IFT144 gene.

Piperno G, Siuda E, Henderson S, Segil M, Vaananen H, Sassaroli M (1998) Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J Cell Biol 143:1591-1601

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

• Locus:
• FLA15 [IFT144]
• Chromosome:
• 13

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

The fla16 mutant was isolated by Piperno in the 137c background, after mutagenesis with nitrosoguanidine. It showed temperature-conditional flagellar assembly. The original fla16 appeared to be unlinked to fla17 (Iomini et al., 2001) but in a later study (2009) Iomini et al. found that the mutant deposited in the stock collection did not recombine with fla17-1. They therefore renamed this strain fla17-3.

Piperno G, Siuda E, Henderson S, Segil M, Vaananen H, Sassaroli M (1998) Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J Cell Biol 143:1591-1601

Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

• Locus:
• FLA17 [IFT139]
• Chromosome:
• 6

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

The fla17 mutant was isolated by Piperno in the 137c background, after mutagenesis with nitrosoguanidine. It shows temperature-conditional flagellar assembly, and was shown to be specifically defective in retrograde intraflagellar transport. Iomini et al. found that it is an in-frame deletion of three exons of the IFT139 gene.

Piperno G, Siuda E, Henderson S, Segil M, Vaananen H, Sassaroli M (1998) Distinct mutants of retrograde intraflagellar transport (IFT) share similar morphological and molecular defects. J Cell Biol 143:1591-1601

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

• Locus:
• FLA17 [IFT139]
• Chromosome:
• 6

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

This is a temperature-conditional mutant with a defect in intraflagellar transport.

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

This is a temperature-conditional flagellar mutant with an alteration in intraflagellar transport.

Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

This mutant is defective in retrograde intraflagellar transport.

Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24

Iomini C, Li L, Esparza JM, Dutcher SK (2009) Retrograde intraflagellar transport mutants identify complex A proteins with multiple genetic interactions in Chlamydomonas reinhardtii. Genetics 183:885-896

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

This mutant is defective in the first phase of intraflagellar transport.

Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24

From Gianni Piperno, Mount Sinai School of Medicine, June 2001

Phenotype: impaired motility

This mutant is defective in anterograde intraflagellar transport.

Iomini C, Babaev-Khaimov V, Sassaroli M, Piperno G (2001) Protein particles in Chlamydomonas flagella undergo a transport cycle consisting of four phases. J Cell Biol 153:13-24

From Ursula Goodenough, Washington University, June 2001

Phenotype: sex reversal

To the best of our knowledge, this is the same as CC-1148, but more recently confirmed by Goodenough.

The imp11 mutant was isolated by Goodenough in the 137c background, and initially characterized as a sex reversal mutant; although isolated in minus, it mated as plus. Subsequently it was found to be a mutation at the MID locus, which is present in the mating-type region only in minus cells and is responsible for minus dominance in mt+/mt- diploids.

Goodenough UW, Detmers PA, Hwang C (1982) Activation for cell fusion in Chlamydomonas: analysis of wild-type gametes and nonfusing mutants. J Cell Biol 92:378-386

Galloway RE, Goodenough UW (1985) Genetic analysis of mating locus linked mutations in Chlamydomonas reinhardii. Genetics 111:447-461

Forest CL (1987) Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 88:613-621

Ferris PJ, Goodenough UW (1997) Mating type in Chlamydomonas is specified by mid, the minus-dominance gene. Genetics 146:859-869

• Locus:
• IMP11 [MID]
• Chromosome:
• 6

From Kyujang Van in Martin Spalding’s laboratory, Iowa State University, August 2001

This is an insertional mutant in the CAH1 gene, isolated by Van and Spalding (1999) in a cw background. This strain was derived by crossing the original mutant to eliminate the cw phenotype.

Van K, Spalding MH (1999) Periplasmic carbonic anhydrase structural gene (Cah1) mutant in chlamydomonas reinhardtii. Plant Physiol 120:757-764

• Locus:
• CAH1
• Chromosome:
• 4

From Patrick Ferris, Goodenough lab, Washington University, August 2001; originally from SAG

Originally obtained from Sammlung von Algenkulturen; this is the strain of C. incerta used for analysis of MID and other mating-type related genes in the Goodenough laboratory. Please see CC-3349 for additional information on C. incerta.

Ferris PJ, Waffenschmidt S, Umen JG, Lin H, Lee JH, Ishida K, Kubo T, Lau J, Goodenough UW (2005) Plus and minus sexual agglutinins from Chlamydomonas reinhardtii. Plant Cell 17:597-615

Lin H, Goodenough UW (2007) Gametogenesis in the Chlamydomonas reinhardtii minus mating type is controlled by two genes, MID and MTD1. Genetics 176:913-925