Strains

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain F60, the original oda3-5 mutant isolate.

Koutoulis A, Pazour GJ, Wilkerson CG, Inaba K, Sheng H, Takada S, Witman GB (1997) The Chlamydomonas reinhardtii ODA3 gene encodes a protein of the outer dynein arm docking complex. J Cell Biol 137:1069-1080

  • Locus:
  • ODA3 [DCC1]
  • Chromosome:
  • 17

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain V64, the original fla14-1 mutant isolate, and was obtained by insertional mutagenesis with the NIT1 gene. Pazour et al. showed that the FLA14 locus corresponds to the gene encoding the 8 kD dynein light chain LC8.

Pazour GJ, Wilkerson CG, Witman GB (1998) A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT). J Cell Biol 141:979-992

  • Locus:
  • FLA14 [DLL1]
  • Chromosome:
  • 3

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain V101, the original fla14-2 mutant isolate, and was obtained by insertional mutagenesis with the NIT1 gene. Pazour et al. showed that the FLA14 locus corresponds to the gene encoding the 8 kD dynein light chain LC8.

Pazour GJ, Wilkerson CG, Witman GB (1998) A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT). J Cell Biol 141:979-992

  • Locus:
  • FLA14 [DLL1]
  • Chromosome:
  • 3

From Gregory Pazour, University of Massachusetts Medical School, February 2002

The fla14-1 mutant (CC-3937) was rescued by transformation with the wild type LC8 gene. This meiotic product has the NIT1 insertion that deleted the original LC8 gene, and the transformed copy of LC8 that has integrated at a site unlinked to the original locus.

Pazour GJ, Wilkerson CG, Witman GB (1998) A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT). J Cell Biol 141:979-992

  • Locus:
  • FLA14 [DLL1]
  • Chromosome:
  • 3

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain F56, the original oda12-1 mutant isolate.

Pazour GJ, Wilkerson CG, Witman GB (1998) A dynein light chain is essential for the retrograde particle movement of intraflagellar transport (IFT). J Cell Biol 141:979-992

  • Locus:
  • ODA12 [DLT2]
  • Chromosome:
  • 12

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain V3, the original oda12-2 mutant isolate.

Pazour GJ, Dickert BL, Witman GB (1999) The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. J Cell Biol 144:473-481

  • Locus:
  • ODA12 [DLT2]
  • Chromosome:
  • 12

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour 2081.2, a product from a cross of the original oda12-1 mutant to wild type.

Pazour GJ, Dickert BL, Witman GB (1999) The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. J Cell Biol 144:473-481

  • Locus:
  • ODA12 [DLT2]
  • Chromosome:
  • 12

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: impaired motility

This is Pazour strain V79, the original ift88-1 mutant isolate.

Pazour GJ, Dickert BL, Vucica Y, Seeley ES, Rosenbaum JL, Witman GB, Cole DG (2000) Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. J Cell Biol 151:709-718

  • Locus:
  • IFT88
  • Chromosome:
  • 7

From Gregory Pazour, University of Massachusetts Medical School, February 2002

The original ift88-1 mutant (CC-3943) was transformed with BAC clone 40-B3 to insert a functional copy of the IFT88 gene. The transformant was then crossed to wild-type to generate Pazour 3276.2, which became CC-3944.

Pazour GJ, Dickert BL, Vucica Y, Seeley ES, Rosenbaum JL, Witman GB, Cole DG (2000) Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. J Cell Biol 151:709-718

  • Locus:
  • IFT88
  • Chromosome:
  • 7

From Gregory Pazour, University of Massachusetts Medical School, February 2002

Phenotype: altered cell shape

This is Pazour strain T103, the original cmu1-1 mutant isolate. This is an insertional mutant with altered cell shape resulting from a mutation affecting cytoplasmic microtubules.

Horst CJ, Fishkind DJ, Pazour GJ, Witman GB (1999) An insertional mutant of Chlamydomonas reinhardtii with defective microtubule positioning. Cell Motil Cytoskeleton 44:143-154

From Patrick Ferris, Washington University, April 2002, his strain K33

Phenotype: requires nicotinamide and thiamine

An imp1 arg2 strain (CC-1865) was transformed with pArg7.8 and a 3.5 kb ApaI fragment containing the MID gene. One of these transformants (B28) was crossed to CC-85 (nic7 mt+) to create a nic7 mt+ strain with a MID transgene. This strain was then crossed to CC-123 (thi10 mt+ ) to create the strains called K33 and L21 (see CC-3948). K33 retains the MID transgene, and although genetically plus, it mates as minus.

  • Locus:
  • NIC7, THI10, MID
  • Chromosome:
  • 6

From Patrick Ferris, Washington University, April 2002

Phenotype: requires nicotinamide and thiamine

An imp1 arg2 strain (CC-1865) was transformed with pArg7.8 and a 3.5 kb ApaI fragment containing the MID gene. One of these transformants (B28) was crossed to CC-85 (nic7 mt+) to create a nic7 mt+ strain with a MID transgene. This strain was then crossed to CC-123 (thi10 mt+ ) to create the strains called K33 (see CC-3947) and L21.

  • Locus:
  • NIC7, THI10
  • Chromosome:
  • 6

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is an ARG7-tagged allele at the CRD1 locus.

Moseley J, Quinn J, Eriksson M, Merchant S (2000) The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii. EMBO J 19:2139-2151

  • Locus:
  • CRD1
  • Chromosome:
  • 7

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is an ARG7-tagged allele at the CRD1 locus.

Moseley J, Quinn J, Eriksson M, Merchant S (2000) The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii. EMBO J 19:2139-2151

  • Locus:
  • CRD1
  • Chromosome:
  • 7

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is an ARG7-tagged allele at the CRD1 locus, with a TOC1-4 transposon insertion (sct1-1) at the CTH1 locus.

Moseley JL, Page MD, Alder NP, Eriksson M, Quinn J, Soto F, Theg SM, Hippler M, Merchant S (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:73-688

  • Locus:
  • CRD1, CTH1
  • Chromosome:
  • 7,12

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is a ble-tagged allele at the CRD1 locus.

Moseley JL, Page MD, Alder NP, Eriksson M, Quinn J, Soto F, Theg SM, Hippler M, Merchant S (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:673-688

  • Locus:
  • CRD1
  • Chromosome:
  • 7

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is a ble-tagged allele at the CRD1 locus, with an insertion, possibly the Gulliver transposon, at the CTH1 locus. CRD1 encodes a di-iron enzyme that is expressed in copper- or oxygen-deficient cells.

Moseley JL, Page MD, Alder NP, Eriksson M, Quinn J, Soto F, Theg SM, Hippler M, Merchant S (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:673-688

  • Locus:
  • CRD1, CTH1
  • Chromosome:
  • 7,12

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: chlorophyll deficient on copper-depleted medium

This is a ble-tagged allele at the CRD1 locus, which encodes a di-iron enzyme that is expressed in copper- or oxygen-deficient cells.

Moseley J, Quinn J, Eriksson M, Merchant S (2000) The Crd1 gene encodes a putative di-iron enzyme required for photosystem I accumulation in copper deficiency and hypoxia in Chlamydomonas reinhardtii. EMBO J 19:2139-2151

Moseley JL, Page MD, Alder NP, Eriksson M, Quinn J, Soto F, Theg SM, Hippler M, Merchant S (2002) Reciprocal expression of two candidate di-iron enzymes affecting photosystem I and light-harvesting complex accumulation. Plant Cell 14:673-688

  • Locus:
  • CRD1
  • Chromosome:
  • 7

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

This is a ble-tagged allele at the CRR1 locus which encodes a copper homeostasis regulator.

Eriksson M, Moseley JL, Tottey S, Del Campo JA, Quinn J, Kim Y, Merchant S (2004) Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes. Genetics 168:795-807

Sommer F, Kropat J, Malasarn D, Grossoehme NE, Chen X, Giedroc DP, Merchant SS (2010) The CRR1 nutritional copper sensor in Chlamydomonas contains two distinct metal-responsive domains. Plant Cell 22:4098-4113

  • Locus:
  • CRR1
  • Chromosome:
  • 2

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: grows poorly and requires acetate on copper-depleted medium

This is a ble-tagged allele at the CRR1 locus which encodes a copper homeostasis regulator.

Eriksson M, Moseley JL, Tottey S, Del Campo JA, Quinn J, Kim Y, Merchant S (2004) Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes. Genetics 168:795-807

Lambertz C, Hemschemeier A, Happe T (2010) Anaerobic expression of the ferredoxin-encoding FDX5 gene of Chlamydomonas reinhardtii is regulated by the Crr1 transcription factor. Eukaryot Cell 9:1747-1754

Sommer F, Kropat J, Malasarn D, Grossoehme NE, Chen X, Giedroc DP, Merchant SS (2010) The CRR1 nutritional copper sensor in Chlamydomonas contains two distinct metal-responsive domains. Plant Cell 22:4098-4113

  • Locus:
  • CRR1
  • Chromosome:
  • 2

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: grows poorey on copper-depleted medium

This is a ble-tagged allele at the CRD2 locus. Mutants at this locus result in copper-conditional iron deficiency.

Eriksson M, Moseley JL, Tottey S, Del Campo JA, Quinn J, Kim Y, Merchant S (2004) Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes. Genetics 168:795-807

  • Locus:
  • CRD2
  • Chromosome:

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

Phenotype: grows poorly on copper-depleted medium

This is a zeocin-resistant strain containing a ble insertion, but crd2-1 is not tagged.

Eriksson M, Moseley JL, Tottey S, Del Campo JA, Quinn J, Kim Y, Merchant S (2004) Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes. Genetics 168:795-807

From Jeffrey Moseley in Sabeeha Merchant’s laboratory, UCLA, June 2002

The sop13 mutation is a suppressor of plastocyanin-deficient mutants, causing these mutants to accumulate cytochrome c6 in copper-containing medium.

Eriksson M, Moseley JL, Tottey S, Del Campo JA, Quinn J, Kim Y, Merchant S (2004) Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes. Genetics 168:795-807

  • Locus:
  • PCY1 [PETE]
  • Chromosome:
  • 3

From William Zerges, Concordia University, October 2002; originally from Michael Timko, University of Virginia

Phenotype: chlorophyll deficient, yellow in the dark

This strain is blocked in both the light and dark pathways of protochlorophyllide conversion. It is light-sensitive, and should be maintained in darkness. The PC1 locus corresponds to the gene encoding NADPH:protochlorophyllide oxidoreductase. The gene represented by the y1 mutation has not yet been identified.

Ford C, Wang W (1980) Three new yellow loci in Chlamydomonas reinhardtii. Mol Gen Genet 179:259-263

Ford C, Mitchell S, Wang W (1983) Characterization of NADPH: Protochlorophyllide oxidoreductase in the y-7 and pc-1 y-7 mutants of Chlamydomonas reinhardtii. Mol Gen Genet 192:290-292

Li J, Timko MP (1996) The pc-1 phenotype of Chlamydomonas reinhardtii results from a deletion mutation in the nuclear gene for NADPH:protochlorophyllide oxidoreductase. Plant Mol Biol 30:15-37

  • Locus:
  • PC1, Y7
  • Chromosome:
  • 1,3

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135