Strains

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond CB, Boynton JE, Gillam NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). VHLR-S4 (CC-3723) and VHLR-S9 (CC-3724) are the best characterized of this series of mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is a spontaneous mutant selected for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). The background strain was the herbicide-resistant mutant psbA-A251L*, obtained from a cross of strain CC-3389 to wild-type CC-124. Please see CC-3389 for more information on the A251L mutation.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is Förster’s VHLR-S1 x 124 II+, a product of the original S1 mutant (CC-2970) to CC-124 wild type.

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is Förster’s VHLR-S1-13 x 124 IV+ , a product of the original S1-13 mutant to CC-124 wild type. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). No genetic analysis has been done to determine whether S-13 is allelic with any of the other VHLR mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is Förster’s VHLR-S1-14 x 124 I-, a product of the original S1-14 mutant to CC-124 wild type. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second). No genetic analysis has been done to determine whether S-14 is allelic with any of the other VHLR mutants.

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is Förster’s VHLR-S5 x 124 III+, a product of the original S5 mutant to CC-124 wild type. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second).

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Britta Förster, Australian National University, January 2003

Phenotype: can grow at very high light intensity

This is Förster’s VHLR-S14 x 124 V-, a product of the original S14 mutant to CC-124 wild type. This is a spontaneous mutant selected, as described by Förster et al. (1999), in the wild-type strain CC-125 for ability to grow and remain green at very high light intensity (1500-2000 micromole photons per square meter per second).

Förster B, Osmond B, Boynton JE, Gillham NW (1999) Mutants of Chlamydomonas reinhardtii resistant to very high light. J Photochem Photobiol 48:127-135

From Irene Baroli and Krishna Niyogi, UC Berkeley, April 2003

Phenotype: carotenoid deficient; requires arginine

The npq2-2 mutant was isolated as a suppressor of the high-light bleaching phenotype of the parent strain npq1 lor1 arg7. Mutants at the NPQ2 locus are deficient in zeaxanthin epoxidase activity. For more information on the NPQ1 and NPQ2 loci, please see Niyogi et al. (1997). For more on lor1, please see CC-2420.

Niyogi KK, Bjorkman O, Grossman AR (1997) Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching. Plant Cell 9:1369-1380

Baroli I, Niyogi KK (2000) Molecular genetics of xanthophyll-dependent photoprotection in green algae and plants. Philos Trans R Soc Lond B Biol Sci 355:1385-1393

Polle JE, Niyogi KK, Melis A (2001) Absence of lutein, violaxanthin and neoxanthin affects the functional chlorophyll antenna size of photosystem-II but not that of photosystem-I in the green alga Chlamydomonas reinhardtii. Plant Cell Physiol 42:482-491

• Locus:
• NPQ1
• Chromosome:
• 2

From Irene Baroli and Krishna Niyogi, UC Berkeley, April 2003

Phenotype: carotenoid deficient; requires arginine

The npq2-3 mutant was isolated as a suppressor of the high-light bleaching phenotype of the parent strain npq1 lor1 arg7-8. Mutants at the NPQ2 locus are deficient in zeaxanthin epoxidase activity. For more information on the NPQ1 and NPQ2 loci, please see Niyogi et al. (1997). For more on lor1, please see CC-2420.

Niyogi KK, Bjorkman O, Grossman AR (1997) Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching. Plant Cell 9:1369-1380

Baroli I, Niyogi KK (2000) Molecular genetics of xanthophyll-dependent photoprotection in green algae and plants. Philos Trans R Soc Lond B Biol Sci 355:1385-1393

Polle JE, Niyogi KK, Melis A (2001) Absence of lutein, violaxanthin and neoxanthin affects the functional chlorophyll antenna size of photosystem-II but not that of photosystem-I in the green alga Chlamydomonas reinhardtii. Plant Cell Physiol 42:482-491

• Locus:
• NPQ1
• Chromosome:
• 2

From Irene Baroli and Krishna Niyogi, UC Berkeley, April 2003

Phenotype: carotenoid deficient; requires arginine

The npq2-4 mutant was isolated as a suppressor of the high-light bleaching phenotype of the parent strain npq1 lor1 arg7-8. Mutants at the NPQ2 locus are deficient in zeaxanthin epoxidase activity. For more information on the NPQ1 and NPQ2 loci, please see Niyogi et al. (1997). For more on lor1, please see CC-2420.

Niyogi KK, Bjorkman O, Grossman AR (1997) Chlamydomonas Xanthophyll Cycle Mutants Identified by Video Imaging of Chlorophyll Fluorescence Quenching. Plant Cell 9:1369-1380

Baroli I, Niyogi KK (2000) Molecular genetics of xanthophyll-dependent photoprotection in green algae and plants. Philos Trans R Soc Lond B Biol Sci 355:1385-1393

Polle JE, Niyogi KK, Melis A (2001) Absence of lutein, violaxanthin and neoxanthin affects the functional chlorophyll antenna size of photosystem-II but not that of photosystem-I in the green alga Chlamydomonas reinhardtii. Plant Cell Physiol 42:482-491

• Locus:
• NPQ1
• Chromosome:
• 2

From Jennifer Keller, Lefebvre lab, University of Minnesota, 2003

Phenotype: antibiotic resistant (cryptopleurine and emetine)

This strain is resistant to cryptopleurine and emetine, and is a missense mutation in the RPS14 gene, encoding cytosolic ribosomal protein S14.

Nelson JA, Savereide PB, Lefebvre PA (1994) The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation. Mol Cell Biol 14:4011-4019

• Locus:
• CRY1 [RPS14]
• Chromosome:
• 11

From Kenneth Hoober, 2003

Phenotype: yellow in the dark

This is Hoober’s isolate of the original y1 mutant, equivalent to CC-735 which was received from Ohad. Please see that strain for more information on this mutation.

• Locus:
• Y1
• Chromosome:
• 15

From James Umen, 2003

Phenotype: abnormal cell division

This is a mat3 allele in the 21 gr background. It can grow on nitrate.

Originally thought to have modified transmission of the chloroplast genome, the mat3 mutants have been shown by Umen and Goodenough to have lesions in a retinoblastoma protein analog involved in cell division. The altered plastid gene transmission is a secondary consequence of abnormally small cell size.

Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Mating type linked mutations which disrupt the uniparental transmission of chloroplast genes in chlamydomonas. Genetics 115:677-684

Armbrust EV, Ibrahim A, Goodenough UW (1995) A mating type-linked mutation that disrupts the uniparental inheritance of chloroplast DNA also disrupts cell-size control in Chlamydomonas. Mol Biol Cell 6:1807-1818

Umen JG, Goodenough UW (2001) Control of cell division by a retinoblastoma protein homolog in Chlamydomonas. Genes Dev 15:1652-1661

• Locus:
• MAT3
• Chromosome:
• 6

From James Umen, 2003

Phenotype: abnormal cell division

Originally thought to have modified transmission of the chloroplast genome, the mat3 mutants have been shown by Umen and Goodenough to have lesions in a retinoblastoma protein analog involved in cell division. The altered plastid gene transmission is a secondary consequence of abnormally small cell size.

Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Mating type linked mutations which disrupt the uniparental transmission of chloroplast genes in chlamydomonas. Genetics 115:677-684

Armbrust EV, Ibrahim A, Goodenough UW (1995) A mating type-linked mutation that disrupts the uniparental inheritance of chloroplast DNA also disrupts cell-size control in Chlamydomonas. Mol Biol Cell 6:1807-1818

Umen JG, Goodenough UW (2001) Control of cell division by a retinoblastoma protein homolog in Chlamydomonas. Genes Dev 15:1652-1661

• Locus:
• MAT3, NIT1
• Chromosome:
• 6,9

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

Phenotype: impaired motility

The oda14 mutants are in the gene encoding protein DC3 of the outer dynein arm-docking complex. This is Casey strain V06, generated by insertional mutagenesis of g1.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

Phenotype: impaired motility

The oda14 mutants are in the gene encoding protein DC3 of the outer dynein arm-docking complex. This is Casey strain V16, generated by insertional mutagenesis of g1.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

Phenotype: impaired motility

The oda14 mutants are in the gene encoding protein DC3 of the outer dynein arm-docking complex. This is Casey strain F28, generated by insertional mutagenesis of 1330.1 (ac14 agg1 nit1 NIT2 mt-) This is NOT the same mutation as the non-photosynthetic mutation F28 studied by Girard et al.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

Phenotype: impaired motility

This is Casey strain 2111.1, from CC-3998 x wild type.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

Phenotype: impaired motility

This is Casey strain V026.2 from V06 [CC-3996] x H8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain t0262.12, from transformation of CC-4000 with an ODA14 genomic clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1