Strains

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain W215, from transformation of CC-4000 with an ODA14 genomic clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain d58, from transformation of strain 026.2 [CC-4000] with a wild type genomic ODA14 clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain d65, from transformation of strain 026.2 [CC-4000] with a wild type genomic ODA14 clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain d76, from transformation of strain 026.2 [CC-4000] with a wild type genomic ODA14 clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain d76, from transformation of strain 026.2 [CC-4000] with a wild type genomic ODA14 clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain e62, from transformation of strain 026.2 [CC-4000] with a modified genomic ODA14 clone and pARG7.8.

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Diane Casey, Witman lab, U. Mass. Medical School, November 2003

This is Casey strain e84, from transformation of strain 026.2 [CC-4000] with a modified genomic ODA14 clone and pARG7.8

Casey DM, Inaba K, Pazour GJ, Takada S, Wakabayashi K, Wilkerson CG, Kamiya R, Witman GB (2003) DC3, the 21-kDa subunit of the outer dynein arm-docking complex (ODA-DC), is a novel EF-hand protein important for assembly of both the outer arm and the ODA-DC. Mol Biol Cell 14:3650-3663

• Locus:
• ODA14
• Chromosome:
• 1

From Peter Luykx, University of Miami, January 2004, his PL40

Phenotype: deficient in osmoregulation

This is an osmoregulatory mutant that grows best on TAP medium supplemented with .06 M sucrose, sorbitol or lactose. It may grow somewhat more slowly than wild type. This strain was obtained in 1995 by UV mutagenesis of CC-125 wild type mt+, and was backcrossed to wild type several times.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL52

Phenotype: deficient in osmoregulation

The osm9 mutant was obtained in 1995 in the laboratory of Greg Pazour, by insertional mutagenesis of a nit1-305 strain originally derived from CC-2453, using a NIT1-containing plasmid pGP505, but the NIT phenotype does not cosegregate with the osm phenotype. Please see CC-4064 for another osm9 isolate that apparently lacks the NIT1 insertion. CC-4010 was obtained by backcrossing the original mutant three times to wild type CC-124 or CC-125 and re-isolating. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL56

Phenotype: deficient in osmoregulation

The osm11 mutant was obtained in 1995 in the laboratory of Greg Pazour, by insertional mutagenesis of a nit1-305 strain originally derived from CC-2453, using a NIT1-containing plasmid pGP505. This strain was obtained by backcrossing the original mutant four times to wild type CC-124 or CC-125 and re-isolating. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL68

Phenotype: deficient in osmoregulation

The osm12 mutant was obtained in 1995 in the laboratory of Greg Pazour, by insertional mutagenesis of a nit1-305 strain originally derived from CC-2453, using a NIT1-containing plasmid pGP505. This strain was obtained by backcrossing the original mutant four times to nit1-305 strain CC-2453. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL69

Phenotype: deficient in osmoregulation

The osm13 mutant was obtained in 1995 in the laboratory of Greg Pazour, by insertional mutagenesis of a nit1-305 strain originally derived from CC-2453, using a NIT1-containing plasmid pGP505. This strain was obtained by backcrossing the original mutant four times. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL96

Phenotype: deficient in osmoregulation

The osm14 mutant was obtained in 1996 from Kosuke Shimogawara, from phosphate-enriched culture of low-phosphate-uptake survivors of a UV-irradiated culture of wild type CC-125. It was backcrossed twice more with wild type CC-124 and re-selected. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL84

Phenotype: deficient in osmoregulation

The osm15 mutant was obtained in 1996 from Kosuke Shimogawara, from phosphate-enriched culture of low-phosphate-uptake survivors of a UV-irradiated culture of wild type CC-125 . It was backcrossed twice more with wild type CC-124 and re-selected. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL85

Phenotype: deficient in osmoregulation

The osm15 mutant was obtained in 1996 from Kosuke Shimogawara, from phosphate-enriched culture of low-phosphate-uptake survivors of a UV-irradiated culture of wild type CC-125 . This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL87

Phenotype: deficient in osmoregulation

The osm16 mutant was obtained in 1996 from Kosuke Shimogawara, from phosphate-enriched culture of low-phosphate-uptake survivors of a UV-irradiated culture of wild type CC-125 . It was backcrossed three times with wild type CC-124 or CC-125 and re-selected. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL86

Phenotype: deficient in osmoregulation

The osm16 mutant was obtained in 1996 from Kosuke Shimogawara, from phosphate-enriched culture of low-phosphate-uptake survivors of a UV-irradiated culture of wild type CC-125. It was backcrossed three times with wild type CC-124 or CC-125 and re-selected.. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL79

Phenotype: deficient in osmoregulation

The osm17 mutant was obtained in 1998 by UV mutagenesis of CC-1690 wild type mt+, backcrossed to wild type CC-1691 and re-isolated. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL100

Phenotype: deficient in osmoregulation

The osm18 mutant was obtained in 1999 by UV-mutagenesis of CC-1691 wild type mt-, backcrossed four times to wild type CC-1690 or CC-1691 and re-isolated. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL101

Phenotype: deficient in osmoregulation

The osm19 mutant was obtained in 1999 by UV-mutagenesis of CC-1690 wild type mt+, backcrossed three times to wild type CC-1690 or CC-1691 and re-isolated. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Peter Luykx, University of Miami, January 2004, his PL102

Phenotype: deficient in osmoregulation

The osm19 mutant was obtained in 1999 by UV-mutagenesis of CC-1690 wild type mt+, backcrossed three times to wild type CC-1690 or CC-1691 and re-isolated. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

Luykx P, Hoppenrath M, Robinson DG (1997) Osmoregulatory mutants that affect the function of the contractile vacuole in Chlamydomonas reinhardtii. Protoplasma 200:99-111

From Maureen Wirschell and George Witman, University of Massachusetts Medical School, March 2004

Phenotype: impaired motility

This is a NIT1 insertion at the ODA5 locus. For more information on the ODA5 locus, please see CC-2236.

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB (2004) Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell 15:2729-2741

• Locus:
• ODA5 [DCC3]
• Chromosome:
• 7

From Maureen Wirschell and George Witman, University of Massachusetts Medical School, March 2004

Phenotype: impaired motility

The oda5-2 mutant is a NIT1 insertion at the ODA5 locus. For more information on the ODA5 locus, please see CC-2236.

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB (2004) Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell 15:2729-2741

• Locus:
• ODA5 [DCC3]
• Chromosome:
• 7

From Maureen Wirschell and George Witman, University of Massachusetts Medical School, March 2004

The oda5-2 mutant is a NIT1 insertion at the ODA5 locus. In this strain the mutant phenotype has been rescued by transformation with the wild type gene. For more information on the ODA5 locus, please see CC-2236.

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB (2004) Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell 15:2729-2741

• Locus:
• ODA5 [DCC3]
• Chromosome:
• 7

From Maureen Wirschell and George Witman, University of Massachusetts Medical School, March 2004

The oda5-2 mutant is a NIT1 insertion at the ODA5 locus. In this strain the mutant phenotype has been rescued by transformation with the wild type gene. For more information on the ODA5 locus, please see CC-2236.

Wirschell M, Pazour G, Yoda A, Hirono M, Kamiya R, Witman GB (2004) Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase. Mol Biol Cell 15:2729-2741

• Locus:
• ODA5 [DCC3]
• Chromosome:
• 7

Chlamydomonas Genetics Center, Duke University, 2004

From a cross of CC-1690 wild type mt+ (21 gr) to CC-1174 y7 mt-

This strain can grow on nitrate, and should be stably green in the dark. CC-4032 is the corresponding mt- strain isolated from the same cross.

Chlamydomonas Genetics Center, Duke University, 2004

From a cross of CC-1690 wild type mt+ (21 gr) to CC-1174 y7 mt-

This cross was made to get a mating type plus and minus pair of strains that were green in the dark and nitrate-utilizing. CC-4031 is the mt+ strain isolated from the same cross.

Chlamydomonas Genetics Center, Duke University, 2004

Phenotype: yellow in the dark

From a cross of CC-1690 wild type mt+ (21 gr) to CC-1170 y5 mt-

This cross was made to get a mating type plus and minus pair of strains that are stably yellow in the dark and can grow on nitrate. CC-4034 is the mt- strain isolated from the same cross.

Please see CC-1169 for more information on the Y5 locus.

• Locus:
• Y5
• Chromosome:
• 1

Chlamydomonas Genetics Center, Duke University, 2004

Phenotype: yellow in the dark

From a cross of CC-1690 wild type mt+ (21 gr) to CC-1170 y5 mt-

This cross was made to get a mating type plus and minus pair of strains that are stably yellow in the dark and can grow on nitrate. CC-4033 is the mt+ strain isolated from the same cross.

Please see CC-1169 for more information on the Y5 locus.

• Locus:
• Y5
• Chromosome:
• 1

From Chiung-Wen Chang in Arthur Grossman’s laboratory, Carnegie Institution of Washington, Stanford, April, 2004

Phenotype: altered phosphate metabolism

Cells of the lpb1 mutant start to die after one day of phosphate deprivation, and viability goes down to about 15% after five days. The specific response for phosphate starvation, including alkaline phosphatase activity and up-regulation of the Ptb2 and Psr1 genes, is similar to wild type. This strain was derived by insertional mutagenesis of CC-425 with pJD67 ARG7, followed by five rounds of back-crossing to wild type CC-125 . The ARG7 insertion site is in the 5532th bp of the genomic clone.

Chang CW, Moseley JL, Wykoff D, Grossman AR (2005) The LPB1 gene is important for acclimation of Chlamydomonas reinhardtii to phosphorus and sulfur deprivation. Plant Physiol 138:319-329

• Locus:
• LPB1
• Chromosome:
• 12