Strains

From Peter Luykx, University of Miami, June 2005, his PL85

Phenotype: deficient in osmoregulation

Please see CC-4020 for more information on this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Peter Luykx, University of Miami, June 2005, his PL87

Phenotype: deficient in osmoregulation

Please see CC-4021 for more information on this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Peter Luykx, University of Miami, June 2005, his PL88

Phenotype: deficient in osmoregulation

Please see CC-4024 for another isolate of this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Peter Luykx, University of Miami, June 2005, his PL124

Phenotype: deficient in osmoregulation

Please see CC-4025 for another isolate of this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Peter Luykx, University of Miami, June 2005, his PL126

Phenotype: deficient in osmoregulation

Please see CC-4026 for another isolate of this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Peter Luykx, University of Miami, June 2005, his PL134

Phenotype: deficient in osmoregulation

This is the osm19 mutant in a nit1 background. Please see CC-4025 for more information on this mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose. This strain has the wild-type allele at the NIT2 locus.

  • Locus:
  • NIT1
  • Chromosome:
  • 9

From Peter Luykx, University of Miami, June 2005, his PL136

Phenotype: deficient in osmoregulation

Please see CC-4025 for more information on the osm19 mutant. This strain should be maintained on TAP medium supplemented with 0.06M sucrose or lactose.

From Krishna Niyogi, UC Berkeley, February 2006

Phenotype: altered chlorophyll fluorescence

This is a null mutation in the LHCBM1 gene. It is deficient in thermal dissipation of excess light energy.

Elrad D, Niyogi KK, Grossman AR (2002) A major light-harvesting polypeptide of photosystem II functions in thermal dissipation. Plant Cell 14:1801-1816

  • Locus:
  • NPQ5 [LHCBM1]
  • Chromosome:
  • 1

From Ritsu Kamiya, University of Tokyo, February 2006

Phenotype: impaired motility

The ida9 mutant is deficient in the dynein heavy chain DHC9.

Yagi T, Minoura I, Fujiwara A, Saito R, Yasunaga T, Hirono M, Kamiya R (2005) An axonemal dynein particularly important for flagellar movement at high viscosity. Implications from a new Chlamydomonas mutant deficient in the dynein heavy chain gene DHC9. J Biol Chem 280:41412-41420

  • Locus:
  • IDA9 [DHC9]
  • Chromosome:
  • 15

From Ritsu Kamiya, University of Tokyo, February 2006

Phenotype: impaired motility

The ida9 mutant is deficient in the dynein heavy chain DHC9.

Yagi T, Minoura I, Fujiwara A, Saito R, Yasunaga T, Hirono M, Kamiya R (2005) An axonemal dynein particularly important for flagellar movement at high viscosity. Implications from a new Chlamydomonas mutant deficient in the dynein heavy chain gene DHC9. J Biol Chem 280:41412-41420

  • Locus:
  • IDA9 [DHC9]
  • Chromosome:
  • 15

From Ritsu Kamiya, University of Tokyo, February 2006

Phenotype: lacks flagella, does not mate

The BLD10 locus encodes a coiled-coil protein localized to the cartwheel structure at the proximal end of the basal body. Null mutants at this locus have no basal bodies or flagella. Alleles in which a truncated protein is made have abnormal basal bodies in which the 9-fold symmetry is altered.

Marshall WF (2007) Centriole assembly: the origin of nine-ness. Curr Biol 7:R1057-R1059

  • Locus:
  • BLD10
  • Chromosome:
  • 10

From Mary Porter, University of Minnesota, April 2006

  • Locus:
  • IDA7
  • Chromosome:
  • 16

From Mary Porter, University of Minnesota, April 2006

Perrone CA, Myster SH, Bower R, O'Toole ET, Porter ME (2000) Insights into the structural organization of the I1 inner arm dynein from a domain analysis of the 1beta dynein heavy chain. Mol Biol Cell 11:2297-2313

  • Locus:
  • IDA2 [DHC10]
  • Chromosome:
  • 14

From Mary Porter, University of Minnesota, April 2006

Perrone CA, Myster SH, Bower R, O'Toole ET, Porter ME (2000) Insights into the structural organization of the I1 inner arm dynein from a domain analysis of the 1beta dynein heavy chain. Mol Biol Cell 11:2297-2313

  • Locus:
  • IDA2 [DHC10]
  • Chromosome:
  • 14

From Mary Porter, University of Minnesota, April 2006

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility. Hendrickson et al. showed that the BOP5 locus encodes the axonemal inner arm I1 intermediate chain dynein IC138, which is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending.

Dutcher SK, Gibbons W, Inwood WB (1988) A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics 120:965-976

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

Hendrickson TW, Perrone CA, Griffin P, Wuichet K, Mueller J, Yang P, Porter ME, Sale WS (2004) IC138 is a WD-repeat dynein intermediate chain required for light chain assembly and regulation of flagellar bending. Mol Biol Cell 15:5431-5442

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

For more information on bop5, see CC-4080.

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

For more information on bop5, see CC-4080.

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

This is the bop5 mutation in a diploid background, originally identified as “diploid colony 2″.

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

This is the bop5 mutation in a diploid background, identified as “diploid colony 4″

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

For more information on bop5, see CC-4080.

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, April 2006

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility.

Dutcher SK, Gibbons W, Inwood WB (1988) A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics 120:965-976

Dutcher SK, Power J, Galloway RE, Porter ME (1991) Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered 82:295-301

  • Locus:
  • BOP2
  • Chromosome:
  • 4

From Mary Porter, University of Minnesota, April 2006

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility.

Dutcher SK, Gibbons W, Inwood WB. A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics. 1988 Dec;120(4):965-76. doi: 10.1093/genetics/120.4.965. PMID: 3224813; PMCID: PMC1203588.

Dutcher SK, Power J, Galloway RE, Porter ME. Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered. 1991 Jul-Aug;82(4):295-301. doi: 10.1093/oxfordjournals.jhered.a111089. PMID: 1880392.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

  • Locus:
  • BOP2, PF10
  • Chromosome:
  • 4,17

From Mary Porter, U of MN, April 2006

Phenotype: slow motility

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME (2019) FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell 30:2659-2680

  • Locus:
  • IDA8/BOP2
  • Chromosome:
  • 4

From Mary Porter, U of MN, April 2006

Phenotype: jiggly motility

 

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME (2019) FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell 30:2659-2680

  • Locus:
  • IDA8/BOP2, PF10
  • Chromosome:
  • 4, 17

From Mary Porter, U of MN, April 2006

Phenotype: jiggly motility

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME (2019) FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell 30:2659-2680

  • Locus:
  • IDA8/BOP2, PF10
  • Chromosome:
  • 4, 17

From Mary Porter, U of MN, April 2006

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Mary Porter, U of MN, April 2006

Phenotype: slow motility

 

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME (2019) FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell 30:2659-2680

  • Locus:
  • DA8/BOP2, ARG7
  • Chromosome:
  • 4, 1

From Mary Porter, U of MN, April 2006

 

Dutcher SK, Gibbons W, Inwood WB. A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics. 1988 Dec;120(4):965-76. doi: 10.1093/genetics/120.4.965. PMID: 3224813; PMCID: PMC1203588.

Dutcher SK, Power J, Galloway RE, Porter ME. Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered. 1991 Jul-Aug;82(4):295-301. doi: 10.1093/oxfordjournals.jhered.a111089. PMID: 1880392.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

From Mary Porter, U of MN, April 2006

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility.

Dutcher SK, Gibbons W, Inwood WB. A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics. 1988 Dec;120(4):965-76. doi: 10.1093/genetics/120.4.965. PMID: 3224813; PMCID: PMC1203588.

Dutcher SK, Power J, Galloway RE, Porter ME. Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered. 1991 Jul-Aug;82(4):295-301. doi: 10.1093/oxfordjournals.jhered.a111089. PMID: 1880392.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Mary Porter, U of MN, April 2006

Phenotype: slow motility

The bop (bypass of paralysis) mutants were isolated by Dutcher et al. in a pf10 background, by selection for restoration of motility.

 

Dutcher SK, Gibbons W, Inwood WB. A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics. 1988 Dec;120(4):965-76. doi: 10.1093/genetics/120.4.965. PMID: 3224813; PMCID: PMC1203588.

Dutcher SK, Power J, Galloway RE, Porter ME. Reappraisal of the genetic map of Chlamydomonas reinhardtii. J Hered. 1991 Jul-Aug;82(4):295-301. doi: 10.1093/oxfordjournals.jhered.a111089. PMID: 1880392.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

  • Locus:
  • IDA8/BOP2, ARG7
  • Chromosome:
  • 4, 1