Strains

From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008

This strain was derived by transformation of lts1-204 with an Rh1-GFP fusion construct (TM1-4) containing Rh1 transmembrane regions 1-4. The lts1 mutant was used as a recipient in order to eliminate chlorophyll fluorescence, which interfered with detection of GFP.

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

Yoshihara C, Inoue K, Schichnes D, Ruzin S, Inwood W, Kustu S (2008) An Rh1-GFP fusion protein is in the cytoplasmic membrane of a white mutant strain of
Chlamydomonas reinhardtii. Mol Plant 1:1007-1020

  • Locus:
  • LTS1 [PSY1]
  • Chromosome:
  • 11

From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008

This strain was derived by transformation of lts1-204 with an Rh1-GFP fusion construct (TM1-6) containing Rh1 transmembrane regions 1-6. The lts1 mutant was used as a recipient in order to eliminate chlorophyll fluorescence, which interfered with detection of GFP.

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

Yoshihara C, Inoue K, Schichnes D, Ruzin S, Inwood W, Kustu S (2008) An Rh1-GFP fusion protein is in the cytoplasmic membrane of a white mutant strain of Chlamydomonas reinhardtii. Mol Plant 1:1007-1020

  • Locus:
  • LTS1 [PSY1]
  • Chromosome:
  • 11

From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008

This strain was derived by transformation of lts1-204 with an Rh1-GFP fusion construct containing the intact Rh1 gene. The lts1 mutant was used as a recipient in order to eliminate chlorophyll fluorescence, which interfered with detection of GFP.

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

Yoshihara C, Inoue K, Schichnes D, Ruzin S, Inwood W, Kustu S (2008) An Rh1-GFP
fusion protein is in the cytoplasmic membrane of a white mutant strain of Chlamydomonas reinhardtii. Mol Plant 1:1007-1020

  • Locus:
  • LTS1 [PSY1]
  • Chromosome:
  • 11

From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008

This strain was derived by transformation of lts1-204 with an Rh1-GFP fusion construct containing the intact Rh1 gene. The lts1 mutant was used as a recipient in order to eliminate chlorophyll fluorescence, which interfered with detection of GFP.

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

Yoshihara C, Inoue K, Schichnes D, Ruzin S, Inwood W, Kustu S (2008) An Rh1-GFP
fusion protein is in the cytoplasmic membrane of a white mutant strain of Chlamydomonas reinhardtii. Mol Plant 1:1007-1020

  • Locus:
  • LTS1 [PSY1]
  • Chromosome:
  • 11

From Corinne Yoshihara, Sydney Kustu lab, University of California, Berkeley, April 2008

Phenotype: carotenoid deficient

Kustu strain CR-521, originally from Niyogi, used as recipient for the transformants CC-4192 – CC-4198. Please see CC-4109 for more information on the LTS1 [PSY1] locus.

McCarthy SS, Kobayashi MC, Niyogi KK (2004) White mutants of Chlamydomonas reinhardtii are defective in phytoene synthase. Genetics 168:1249-1257

  • Locus:
  • LTS1 [PSY1]
  • Chromosome:
  • 11

From Brian Piasecki, University of Minnesota, May 2008

Piasecki BP, Silflow CD (2008) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378

From Brian Piasecki, University of Minnesota, May 2008

Piasecki BP, Silflow CD (2008) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378

From Brian Piasecki, University of Minnesota, May 2008

Piasecki BP, Silflow CD (2008) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378

From Brian Piasecki, University of Minnesota, May 2008

Piasecki BP, Silflow CD (2008) The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas. Mol Biol Cell 20:368-378

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE], PF15 [KAT2]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE], PF15 [KAT2]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE], PF15 [KAT2]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD2 [TUE]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • BLD10, PF15 [KAT2]
  • Chromosome:
  • 3,10

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • PF15 [KAT2]
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • PF15 [KAT2], UNI1
  • Chromosome:
  • 3,17

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • PF15 [KAT2], UNI3
  • Chromosome:
  • 3

From Susan Dutcher, Washington University, September 2008

  • Locus:
  • PF15 [KAT2], VFL1
  • Chromosome:
  • 3,8

From Christoph Beck, University of Freiburg (Albert-Ludwigs-Universität Freiburg), October 2008

This is a mutant generated by UV mutagenesis of the 4A+ strain (CC-4051) and was determined to be a mutation in the CHLH gene. It is light-sensitive and brown in the dark.

von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567

  • Locus:
  • CHLH
  • Chromosome:
  • 7

From Christoph Beck, University of Freiburg (Albert-Ludwigs-Universität Freiburg), October 2008

This mutant was generated by UV mutagenesis of the A4+ strain (CC-4051) and was determined to be a mutation in the CHLD gene. It is light-sensitive and brown in the dark.

von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567

  • Locus:
  • CHLD
  • Chromosome:
  • 5

From Christoph Beck, University of Freiburg (Albert-Ludwigs-Universität Freiburg), October 2008

This mutant was generated by UV mutagenesis of the A4+ strain (CC-4051) and was determined to be a mutation in the CHLD gene. It is light-sensitive and brown in the dark.

von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567

  • Locus:
  • CHLD
  • Chromosome:
  • 5

From Christoph Beck, University of Freiburg (Albert-Ludwigs-Universität Freiburg), October 2008

This is a mutant generated by UV mutagenesis of the 4A+ strain (CC-4051) as described by von Gromoff et al. It is defective in Mg-protoporphyrin IX methyltransferase (MgPMT). It is yellow in the dark and green when grown in dim to medium light. At high light intensities it is yellow.

von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567

Meinecke L, Alawady A, Schroda M, Willows R, Kobayashi MC, Niyogi KK, Grimm B, Beck CF (2010) Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant Mol Biol 72:643-658

  • Locus:
  • CHLM
  • Chromosome:
  • 12

From Christoph Beck, University of Freiburg (Albert-Ludwigs-Universität Freiburg), October 2008

This is a mutant generated by UV mutagenesis of the 4A+ strain (CC-4051) as described by von Gromoff et al. It is defective in Mg-protoporphyrin IX methyltransferase (MgPMT). It is yellow in the dark or dim light and dies in medium to bright light.

von Gromoff ED, Alawady A, Meinecke L, Grimm B, Beck CF (2008) Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas. Plant Cell 20:552-567

Meinecke L, Alawady A, Schroda M, Willows R, Kobayashi MC, Niyogi KK, Grimm B, Beck CF (2010) Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant Mol Biol 72:643-658

  • Locus:
  • CHLM
  • Chromosome:
  • 12

From Lai-Wa Tam, Lefebvre lab, University of Minnesota, October 2008

James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics. 118, 141-147

James SW, Lefebvre PA (1992) Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics 130:305-314

Silflow CD, Sun X, Haas NA, Foley JW, Lefebvre PA (2011) The Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas. Genetics 189:1249-60

  • Locus:
  • APM1, NIT1
  • Chromosome:
  • 17,9