Strains
CC-4358 sultr2 mt+
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This mutant was obtained by insertional mutagenesis of CC-1690, and recovered as a transformant that failed to accumulate the SULTR2 sulfate transporter when starved for sulfur. This strain is paromomycin resistant.
Pootakham W, Gonzalez-Ballester D, Grossman AR (2010) Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas. Plant Physiol 153:1653-1668
CC-4359 slt1 slt2
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This is a product from a cross of slt1 x slt2.
Pootakham W, Gonzalez-Ballester D, Grossman AR (2010) Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas. Plant Physiol 153:1653-1668
CC-4360 slt1 sultr2
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This is a product from a cross of slt1 x sultr2
Pootakham W, Gonzalez-Ballester D, Grossman AR (2010) Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas. Plant Physiol 153:1653-1668
CC-4361 slt2 sultr2
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This is a product from a cross of slt2 x sultr2
Pootakham W, Gonzalez-Ballester D, Grossman AR (2010) Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas. Plant Physiol 153:1653-1668
CC-4362 slt1 slt2 sultr2
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This is a product from a series of crosses between slt1, slt2, and sultr2.
Pootakham W, Gonzalez-Ballester D, Grossman AR (2010) Identification and regulation of plasma membrane sulfate transporters in Chlamydomonas. Plant Physiol 153:1653-1668
CC-4363 ars73a
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This strain was obtained by insertional mutagenesis in a nit2 cw15 (D66) background.
Aksoy M, Pootakham W, Pollock SV, Moseley JL, Gonzalez-Ballester D, Grossman AR (2013) Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor. Plant Physiol. 162:195-211
CC-4364 sac3 ars73a
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, July 2010
This strain was obtained from a cross between sac3 and ars73a mutants.
Aksoy M, Pootakham W, Pollock SV, Moseley JL, Gonzalez-Ballester D, Grossman AR (2013) Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor. Plant Physiol. 162:195-211
CC-4365 ars11 ars73a
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, August 2010
This strain was obtained from a cross between ars11 (snrk2.1) and ars73a mutants.
Aksoy M, Pootakham W, Pollock SV, Moseley JL, Gonzalez-Ballester D, Grossman AR (2013) Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor. Plant Physiol. 162:195-211
CC-4366 sac1 ars73a
$30.00
$30.00
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, August 2010
This strain was obtained from a cross between sac1 and ars73a mutants.
Aksoy M, Pootakham W, Pollock SV, Moseley JL, Gonzalez-Ballester D, Grossman AR (2013) Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor. Plant Physiol. 162:195-211
From Nik Pootakham, Arthur Grossman lab, Carnegie Institution, Stanford CA, August 2010
This strain was obtained by complementing ars73a mutant with BAC PTQ14775 (co-transformation with bleomycin-resistant marker).
Aksoy M, Pootakham W, Pollock SV, Moseley JL, Gonzalez-Ballester D, Grossman AR (2013) Tiered regulation of sulfur deprivation responses in Chlamydomonas reinhardtii and identification of an associated regulatory factor. Plant Physiol. 162:195-211
From David L. Herrin, University of Texas at Austin, September 2010, his 7151
The css1 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (CC-1021, also CC-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.
Li F, Holloway SP, Lee J, Herrin DL (2002) Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii. Plant J 32:467-480
Liu Y, Lee D, Ialicicco M, Akkinepalli H, Morreale G, Leung H, Scippa GS, Greenland A, Mackay I (2012) Genotyping SSR length variants by isothermal DNA amplification. Genome 55:691-695
From David L. Herrin, University of Texas at Austin, September 2010, his 7120
The css2 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (CC-1021, also CC-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.
Li F, Holloway SP, Lee J, Herrin DL (2002) Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii. Plant J 32:467-480
Liu Y, Lee D, Ialicicco M, Akkinepalli H, Morreale G, Leung H, Scippa GS, Greenland A, Mackay I (2012) Genotyping SSR length variants by isothermal DNA amplification. Genome 55:691-695
From David L. Herrin, University of Texas at Austin, September 2010, his 71N1
The css3 mutation suppresses defects in splicing of the chloroplast 23S (Cr.LSU) intron and also the psbA fourth intron. It was obtained as follows: Mets strain 2137 (CC-1021, also CC-3269) was transformed with a construct that contained the spr-u-1-6-2 mutation from plasmid P-183 (a single base pair change in the 16S rRNA) and the chloroplast 23S (Cr.LSU) intron, in which site-directed changes had been made. Transformants were selected by spectinomycin resistance. A transformant defective in Cr.LSU self-splicing (P4125A), which grew slowly and was light-sensitive, was then used to isolate suppressor mutations that restored Cr.LSU splicing.
Li F, Holloway SP, Lee J, Herrin DL (2002) Nuclear genes that promote splicing of group I introns in the chloroplast 23S rRNA and psbA genes in Chlamydomonas reinhardtii. Plant J 32:467-480
Liu Y, Lee D, Ialicicco M, Akkinepalli H, Morreale G, Leung H, Scippa GS, Greenland A, Mackay I (2012) Genotyping SSR length variants by isothermal DNA amplification. Genome 55:691-695
CC-4371 bbs1-1 mt-
$30.00
$30.00
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their RIR7-2
This is a previously uncharacterized mutant obtained from Carol Dieckmann, University of Arizona. The parent of the strain is not certain, an e-mail from Dr. Dieckmann indicates that the strain was probably generated by transformation with the pArg 7.8 plasmid that was linearized with EcoR1 before transformation (hence designation R1_). Arg+ transformants were generated by the silicon carbide whisker method. This strain is phototaxis negative and has good motility.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their BBS4-GFP
This is a rescue of bbs4-1 (CC-4377) with a GFP epitope tag. It is phototaxis and photoshock positive.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their BBS4HA21
This is a rescue of bbs4-1 (CC-4377) with the HA epitope tag. It is phototaxis and photoshock positive.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their F2D2
This is a mutant in which most of the CEP290 gene has been deleted. The Y168 stain (cep290-1:: NIT1, nit1,agg1,mt+) was obtained by insertional mutagenesis of the g1 strain ( (nit1, agg1, mt+; see Pazour et al. 1995) with the NIT1 plasmid pGP505. The cep290-1 strain was obtained by backcrossing the original mutant Y168 to CC-124. A mutant progeny from this first cross was backcrossed again to g1, and a mutant progeny from the second cross was designated as cep290-1.
Mutant cells are mostly palmelloid and have very short/stumpy flagella once released from the mother cell wall by treatment with autolysin.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
CC-4375 ift46-1::NIT1 mt+
$30.00
$30.00
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their YH6
This is an insertional mutation in the IFT46 gene. The cells are mostly palmelloid and have short, paralyzed flagella lacking dynein arms and with central pair defects.
The T8a4-11 stain (ift46-1::NIT1, nit1, pf1, mt+) was obtained by insertional mutagenesis of the KK30A3 strain ((nit1, pf1, mt+) with the NIT1 plasmid pMN24 by K.Kozminski and J. Rosenbaum (Yale University, New Haven, CT). The ift46-1 strain was obtained by crossing the original mutant T8a4-11 to CC-124 to get rid of the pf1 mutation.
Hou Y, Qin H, Follit JA, Pazour GJ, Rosenbaum JL, Witman GB (2007) Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. J Cell Biol 176:653-665
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their MxG1.3
This strain was obtained by crossing BBS4-GFP (CC-4372) to IFT20mCherry.
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their T32
This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with the NIT1 plasmid pMN24. It is phototaxis negative, photoshock positive. Lechtreck et al. showed that it is an allele at the BBS4 locus.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131, 427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187, 1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their V72
This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with pGP505. It is phototaxis negative, photoshock positive. Lechtreck et al. showed that it is an allele at the BBS4 locus.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their V84
This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with pGP505. It is phototaxis negative, photoshock positive. Lechtreck et al. showed that it is an allele at the BBS4 locus.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their F36
This mutant was obtained by transformation of strain 1330.1 (ac14 agg1 nit1 NIT2 mt) with pGP505m abd was characterized as “flagellar current (FC) positive, photo receptor current (PCR) positive.” Lechtreck et al. showed that it is an allele at the BBS4 locus.
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their T87
This mutant was obtained by transformation of strain g1 (nit1, agg1, mt+) with the NIT1 plasmid pMN24. It is phototaxis negative, photoshock positive. Lechtreck et al. showed that it is an allele at the BBS7 locus.
Witman included the following note: “The ptx6-1 (T197) and ptx6-2 (T87) strains in the Witman collection now (2010) appear to be identical. Hence, Lechtreck et al. (2010) equated bbs7-1 with ptx6-1, although the strain used was T87 (ptx6-2). The mutation in the BBS7 gene of T87 involves a retrotransposon TOC1 footprint whereas Pazour et al. (1995) reported that the ptx6-1 strain contained pUC119 sequence that segregated with the nonphototactic phenotype but did not encode a functional nitrate reductase gene. It is not known if the bbs7-1 strain (i.e., T87) carries a second, insertional, mutation linked to the BBS4 gene, or if the original ptx6-1 and ptx6-2 alleles were different but ptx6-1 has been lost. In any case, this strain should be referred to simply as bbs7-1. If relevant, anyone working with this strain in the future should check to see if it still contains pUC119 sequence that might be disrupting a second gene.”
Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440
Lechtreck KF, Johnson EC, Sakai T, Cochran D, Ballif BA, Rush J, Pazour GJ, Ikebe M, Witman GB (2009) The Chlamydomonas reinhardtii BBSome is an IFT cargo required for export of specific signaling proteins from flagella. J Cell Biol 187:1117-1132
From Deborah Cochran, George Witman lab, University of Massachusetts Medical School, November 2010, their CYH6
Supift461 is a spontaneous partial suppressor for ift46-1 (CC-4375).
Cells of this strain are mostly palmelloid and have very short/stumpy flagella. However under certain growth conditions, the cells can manage to assemble flagella up to wild-type length and swim slowly.
Hou Y, Qin H, Follit JA, Pazour GJ, Rosenbaum JL, Witman GB (2007) Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella. J Cell Biol 176:653-665
From Paul A. Lefebvre, University of Minnesota, November 2010
This is a very tight flagella-less mutant, created by insertional mutagenesis with the NIT1 gene (pMN24) in the A54-e18 background. (CC-2929).
Brazelton WJ, Amundsen CD, Silflow CD, Lefebvre PA (2001) The bld1 mutation identifies the Chlamydomonas osm-6 homolog as a gene required for flagellar assembly. Curr Biol 11:1591-1594
CC-4385 psbD (deletion) mt+
$30.00
$30.00
From Tasios Melis, University of California, Berkeley, November 2010; originally from Dwight Barnes and Stephen Mayfield
This mutant, which was isolated in the CC-125 background, lacks the psbD gene and is unable to synthesize the D2 reaction center protein of photosystem II. In consequence, it cannot assemble any of the PSII-RC proteins, and cannot do PSII photochemistry. It requires acetate for growth.
CC-4386 rep27 (deletion) mt+
$30.00
$30.00
From Tasios Melis, University of California, Berkeley, November 2010
This strain was obtained by insertional mutagenesis of CC-425 with the pJD67 (ARG7) plasmid. it lacks the REP27 gene, and is unable to repair PSII from photo-oxidative damage. It cannot complete the insertion of a nascent D1 into the PSII template during turnover of the D1/32 kD reaction center protein of PSII. This strain requires acetate for growth.
Park S, Khamai P, Garcia-Cerdan JG, Melis A (2007) REP27, a tetratricopeptide repeat nuclear-encoded and chloroplast-localized protein, functions in D1/32-kD reaction center protein turnover and photosystem II repair from photodamage. Plant Physiol 143:1547-1560
Dewez D, Park S, García-Cerdán JG, Lindberg P, Melis A (2009) Mechanism of REP27 protein action in the D1 protein turnover and photosystem II repair from photodamage. Plant Physiol 151:88-99
CC-4387 rep55 mt+
$30.00
$30.00
From Tasios Melis, University of California, Berkeley, November 2010
This mutant was obtained by insertional mutagenesis with pJD67. It has leaky expression of the chloroplast sulfate permease (SULP; see references below), limited sulfate uptake by the chloroplast and limited capacity for the photosystem II repair process.
Chen HC, Yokthongwattana K, Newton AJ, Melis A (2003) SulP, a nuclear gene encoding a putative chloroplast-targeted sulfate permease in Chlamydomonas reinhardtii. Planta 218:98-106
Chen HC, Melis A (2004) Localization and function of SulP, a nuclear-encoded chloroplast sulfate permease in Chlamydomonas reinhardtii. Planta 220:198-210
CC-4388 psbH::aadA mt+
$30.00
$30.00
From Saul Purton, University College London, November 2012
Chloroplast transformant in which the aadA (SpcR) cassette has been inserted into the BstXI site in the middle of the psbH coding sequence. The aadA gene is in the same orientation as psbH.
O'Connor HE, Ruffle SV, Cain AJ, Deak Z, Vass I, Nugent JH, Purton S (1988) The 9-kDa phosphoprotein of photosystem II. Generation and characterisation of Chlamydomonas mutants lacking PSII-H and a site-directed mutant lacking the phosphorylation site. Biochim Biophys Acta 1364:63-72
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