Strains

From Mary Porter, University of Minnesota, March 14, 2011

This bop5 allele was isolated and characterized in the Kamiya lab, and the mutation was identified in the Porter lab.

Ikeda K, Yamamoto R, Wirschell M, Yagi T, Bower R, Porter ME, Sale WS, Kamiya R (2009) A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 66:448-456

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This bop5 allele was isolated and characterized in the Kamiya lab, and the mutation was identified in the Porter lab.

Ikeda K, Yamamoto R, Wirschell M, Yagi T, Bower R, Porter ME, Sale WS, Kamiya R (2009) A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil. Cytoskeleton 66:448-456

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This bop5 allele was isolated and characterized in the Kamiya lab, and the mutation was identified in the Porter lab.

Ikeda K, Yamamoto R, Wirschell M, Yagi T, Bower R, Porter ME, Sale WS, Kamiya R (2009) A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 66:448-456

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This bop5 allele was isolated and characterized in the Kamiya lab, and the mutation was identified in the Porter lab.

Ikeda K, Yamamoto R, Wirschell M, Yagi T, Bower R, Porter ME, Sale WS, Kamiya R (2009) A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 66:448-456

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This bop5 allele was isolated and characterized in the Kamiya lab, and the mutation was identified in the Porter lab.

Ikeda K, Yamamoto R, Wirschell M, Yagi T, Bower R, Porter ME, Sale WS, Kamiya R (2009) A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. Cell Motil Cytoskeleton 66:448-456

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of mia1-1 mt+ x bop5-1 mt-, by K. VanderWaal in the Porter lab.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], MIA1
  • Chromosome:
  • 2,12

From Mary Porter, University of Minnesota, March 14, 2011

This strain is a rescue of the bop5-3 phenotype by co-transformation with pHX (containing IC138) and pSI103 into bop5-3 (807) mt+

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138]
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of bop5-3 (807) mt+ to mia2 mt- by R. Kamiya.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], MIA2
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of bop5-3 (807) mt+ to mbo2 mt- by K. VanderWaal.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], MBO2
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of bop5-3 (807) mt+ to pf10 mt- by K. VanderWaal.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], PF10
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of bop5-3 (807) mt+ to uni1-2 mt- by K. VanderWaal.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], UNI1
  • Chromosome:
  • 12

From Mary Porter, University of Minnesota, March 14, 2011

This is a product from a cross of bop5-4 (815) mt- to mia1 mt+ by R. Kamiya.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • BOP5 [IC138], MIA1
  • Chromosome:
  • 2,12

From Mary Porter, University of Minnesota, March 14, 2011

This strain was obtained by transformation of ida2-7 with a fragment of the DHC10 gene (pCAP1) encoding the N-terminal domain and pSI103, resulting in partial rescue of the mutant phenotype.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

  • Locus:
  • IDA2 [DHC10]
  • Chromosome:
  • 14

From Susan Dutcher, Washington University, March 2011

This strain, which carries the agg1, nit1 and nit2 alleles, was obtained from a cross of CC-124 x CC-125, followed by a series of 10 backcrosses to CC-124, carried out for three months beginning in December 2010. It has greater than 99% viability out of meiosis, and it mates out of low sulfate plates and M-N/5 after 2 hours.

From Susan Dutcher, Washington University, March 2011

This strain, which carries the agg1, nit1 and nit2 alleles, was obtained from a cross of CC-124 x CC-125, followed by a series of 10 backcrosses to CC-124, carried out for three months beginning in December 2010. It has greater than 99% viability out of meiosis, and it mates out of low sulfate plates and M-N/5 after 2 hours.

From Mary Porter, University of Minnesota, April 27, 2011

This pf2 allele was generated by insertion of a NIT1 plasmid (pMN24) into the PF2 gene. Background strain is nit1-305 mt-.

Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57

  • Locus:
  • PF2
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, April 27, 2011

This rescued strain was generated by transformation with PF2-GFP and pSI103 with selection on paromomycin. Screened for rescue of motility and presence of GFP signal in the flagella. Strong PF2-GFP signal found in flagella.

Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF2
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, April 27, 2011

This rescued strain was created by transformation of sup-pf-3 mt- (CC-1399) with PF2-GFP and pSI103 with selection on paromomycin. Screened for rescue of motility and presence of GFP signal in the flagella. Strong PF2-GFP signal in flagella, weak expression of sup-pf-3 gene products (truncated PF2) by Western blot.

Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-24

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • SUP-PF3
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, April 27, 2011

This rescued strain was created by transformation of sup-pf-3 mt- (CC-1399) with PF2-GFP and pSI103 with selection on paromomycin. Screened for rescue of motility and presence of GFP signal in the flagella. Strong PF2-GFP signal in flagella, weak expression of sup-pf-3 gene products (truncated PF2) by Western blot.

Huang B, Ramanis Z, Luck DJ (1982) Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function. Cell 28:115-24

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • SUP-PF3
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, April 27, 2011

pf14 was crossed to pf2-4::PF2-GFP and tetrad progeny were scored for the PF14 (RSP3) and PF2 (DRC4) gene products by Western blot. This strain has the mutant pf14 and pf2 alleles, but also expresses the PF2-GFP construct.

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF2, PF14
  • Chromosome:
  • 6,11

From Mary Porter, University of Minnesota, April 27, 2011

pf14 was crossed to pf2-4::PF2-GFP and tetrad progeny were scored for the PF14 (RSP3) and PF2 gene products (DRC4) by Western blot. This strain has the pf2 allele, but also expresses the PF2-GFP construct. It is wild-type for PF14 (RSP3). Is has a strong PF2-GFP signal in flagella and the mating type is unknown.

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF2, PF14
  • Chromosome:
  • 6,11

From Mary Porter, University of Minnesota, April 27, 2011

pf14 was crossed to pf2-4::PF2-GFP and tetrad progeny were scored for the PF14 (RSP3)and PF2 (DRC4) gene products by Western blot. This strain has the mutant pf14 and pf2 alleles, but also expresses the PF2-GFP construct. It has a strong PF2-GFP signal in the flagella, pf14 paralysis and the mating type unknown.

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF2, PF14
  • Chromosome:
  • 6,11

From Mary Porter, University of Minnesota, April 27, 2011

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF2, PF14
  • Chromosome:
  • 6,11

From Mary Porter, University of Minnesota, April 27, 2011

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF14
  • Chromosome:
  • 6

From Mary Porter, University of Minnesota, April 27, 2011

pf2-4 was crossed to an arg7 strain and transformed with pARG7.8 and an HA-tagged copy of the wild-type PF2 gene. Motility is restored to wild-type.

Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57

  • Locus:
  • PF2
  • Chromosome:
  • 11

From David Nelson, Lefebvre lab, University of Minnesota, May 2011

DN2 is a sub-species of Chlamydomonas reinhardtii isolated from an environmental sample taken at 13,000 feet in Breckenridge, Colorado by David Nelson (Lefebvre lab, U of MN). The sample was collected Friday, August 10, 2007 at 12:00 PM. DN2 exhibits a large size compared to CC-124 and can grow at low temperatures. It has been grown =at 4 degrees Celsius.

From Robert Spreitzer, Univ. of Nebraska, May 2011

In this strain, both RBCS1 and RBCS2 have been disrupted by insertional mutagenesis. This is the background strain for CC-3875 through CC-3893, in which constructs with modified RBCS genes were introduced by Boschetti and collaborators.

Khrebtukova I, Spreitzer RJ (1996b) Elimination of the Chlamydomonas gene family that encodes the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc Natl Acad Sci USA 93:13689-13693

From Mary Porter, University of Minnesota, May 2011

A backcross of the original bop5-2 mutant (CC-4284) to CC-4264 apml-19 nit1 mt- (L8) by C. Perrone in the Porter lab.

Bower R, VanderWaal K, O'Toole E, Fox L, Perrone C, Mueller J, Wirschell M, Kamiya R, Sale WS, Porter ME (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Mol Biol Cell 20:3055-3063

From Jean-David Rochaix, University of Geneva, May 31, 2011

This nuclear mutation affects the stability of transcripts of the chloroplast-encoded psbD gene, resulting in degradation of the photosystem II reaction center complex.

Kuchka MR, Goldschmidt-Clermont M, van Dillewijn J, Rochaix JD (1989) Mutation at the Chlamydomonas nuclear NAC2 locus specifically affects stability of the chloroplast psbD transcript encoding polypeptide D2 of PS II. Cell 58:869-876

  • Locus:
  • NAC2 [MBD1]
  • Chromosome:
  • 10

From Benjamin Engel and Wallace Marshall, UCSF, June 28, 2011

This is an A to T transversion at position 12313 of the DHC1b coding sequence that results in an Ile to Phe substitution at amino acid 4105. The mutation was UV-induced in the CC-125 background and the original strain was back-crossed twice to eliminate additional mutations that might have resulted from the UV exposure.

Engel BD, Ishikawa H, Wemmer KA, Geimer S, Wakabayashi K, Hirono M, Craige B, Pazour GJ, Witman GB, Kamiya R, Marshall WF (2012) The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol 199:151-167

Shih SM, Engel BD, Kocabas F, Bilyard T, Gennerich A, Marshall WF, Yildiz A (2013) Intraflagellar transport drives flagellar surface motility. ELife 2

  • Locus:
  • DHC1b
  • Chromosome:
  • 6