Strains

From Benjamin Engel and Wallace Marshall, UCSF, June 28, 2011

This is an A to T transversion at position 12313 of the DHC1b coding sequence that results in an Ile to Phe substitution at amino acid 4105. The mutation was UV-induced in the CC-125 background, and the original strain was back-crossed twice to eliminate additional mutations that might have resulted from the UV exposure.

This strain was generated in the course of tetrad analysis for mapping purposes. The DHC1b locus is very close to the mating type locus, with recombination between the two of only about 1%.

Engel BD, Ishikawa H, Wemmer KA, Geimer S, Wakabayashi K, Hirono M, Craige B, Pazour GJ, Witman GB, Kamiya R, Marshall WF (2012) The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol 199:151-167

Shih SM, Engel BD, Kocabas F, Bilyard T, Gennerich A, Marshall WF, Yildiz A (2013) Intraflagellar transport drives flagellar surface motility. ELife 2

  • Locus:
  • DHC1b
  • Chromosome:
  • 6

From Benjamin Engel and Wallace Marshall, UCSF, June 28, 2011

This strain was generated by mating dhc1b-3 mt+ (see CC-4422) to fla10 mt-. It is temperature sensitive, losing its flagella at 34ûC with similar kientics to fla10.

Engel BD, Ishikawa H, Wemmer KA, Geimer S, Wakabayashi K, Hirono M, Craige B, Pazour GJ, Witman GB, Kamiya R, Marshall WF (2012) The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function. J Cell Biol 199:151-167

  • Locus:
  • DHC1b, FLA10 [KHP1]
  • Chromosome:
  • 6,17

From Wenqiang Yang in Arthur Grossman’s lab, Carnegie Institution, Stanford, July 8, 2011

This is a cw15 nit1 strain suitable for transformation. D66 is a product from the fifth consecutive cross in which meiotic progeny containing the cell wall mutation cw15 ,were successively backcrossed to strains A33, A53, 305, B27, B26 and A35 (Schnell and Lefebvre, 1993).

Schnell RA, Lefebvre PA (1993) Isolation of the Chlamydomonas regulatory gene NIT2 by transposon tagging. Genetics 134:737-747

Pollock SV, Colombo SL, Prout DL Jr, Godfrey AC, Moroney JV (2003) Rubisco activase is required for optimal photosynthesis in the green alga Chlamydomonas reinhardtii in a low-CO(2) atmosphere. Plant Physiol 133:1854-1861

  • Locus:
  • NIT2
  • Chromosome:
  • 3

From George Witman, University of Massachusetts Medical School, July 13, 2011, his strain 3167.2

The oda15-1 mutant (V38) was isolated in the g1 background (nit1, agg1, mt+) of Pazour et al., 1995, by transformation with pGP505 Strain 3167.2 was derived by crossing V38 to CC-124 (DiBella et al., 2004).

Pazour GJ, Sineshchekov OA, Witman GB (1995) Mutational analysis of the phototransduction pathway of Chlamydomonas reinhardtii. J Cell Biol 131:427-440

Pazour GJ, Witman GB (2000) Forward and reverse genetic analysis of microtubule motors in Chlamydomonas. Methods 22:285-298

DiBella LM, Sakato M, Patel-King RS, Pazour GJ, King SM (2004) The LC7 light chains of Chlamydomonas flagellar dyneins interact with components required for both motor assembly and regulation. Mol Biol Cell 15:4633-4646

  • Locus:
  • ODA15 [DLC7a, DLR1]
  • Chromosome:
  • 8

From Yingjun Wang and Martin Spalding, Iowa State University, July 20, 2011

This is the original cia5 mutation, previously designated P34. Please see CC-2702 for more information on this locus.

  • Locus:
  • CIA5 [CCM1]
  • Chromosome:
  • 2

From Yingjun Wang and Martin Spalding, Iowa State University, July 20, 2011

This is a deletion mutation in the CAH3 gene, and is allelic to CC-1219. Wang has designated this cah3-124C. Please see CC-2699 for more information on the CAH3 locus.

  • Locus:
  • CAH3
  • Chromosome:
  • 9

From Yingjun Wang and Martin Spalding, Iowa State University, July 20, 2011

This strain has a deletion of two HSP22 heat shock proteins, and was originally isolated as a mutant requiring high CO2, designated HCR3510 or HSP3510. It has been suggested that the two HSP proteins deleted here might be involved in photoprotection.

From Yingjun Wang and Martin Spalding, Iowa State University, July 20, 2011

This is Wang’s strain HCR301, isolated as a mutant requiring high CO2, and subsequently identified as an insertional mutation near the men-D gene, encoding an enzyme in the phylooquinone biosynthetic pathway. A similar mutant was described by Lefebvre-Legendre et al. but has not been compared directly to this allele yet.

Lefebvre-Legendre L, Rappaport F, Finazzi G, Ceol M, Grivet C, Hopfgartner G, Rochaix JD (2007) Loss of phylloquinone in Chlamydomonas affects plastoquinone pool size and photosystem II synthesis. J Biol Chem 282:13250-13263

From Carolyn Silflow, University of Minnesota, July 2011

Mutation in the DNAJ1 gene.

James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics 118, 141-147

James SW, Lefebvre PA (1992) Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics 130:305-314

Silflow CD, Sun X, Haas NA, Foley JW, Lefebvre PA (2011) The Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas. Genetics 189:1249-60

  • Locus:
  • APM1
  • Chromosome:
  • 17

From Carolyn Silflow, University of Minnesota, July 2011

Mutation in the DNAJ1 gene.

James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics 118, 141-147

James SW, Lefebvre PA (1992) Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics 130:305-314

Silflow CD, Sun X, Haas NA, Foley JW, Lefebvre PA (2011) The Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas. Genetics 189:1249-60

  • Locus:
  • APM1
  • Chromosome:
  • 17

From Carolyn Silflow, University of Minnesota, July 2011

Mutation in the DNAJ1 gene.

James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics 118:141-147

James SW, Lefebvre PA (1992) Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics 130:305-314

Silflow CD, Sun X, Haas NA, Foley JW, Lefebvre PA (2011) The Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas. Genetics 189:1249-60

  • Locus:
  • APM1
  • Chromosome:
  • 17

From Carolyn Silflow, University of Minnesota, July 2011

Mutation in the DNAJ1 gene.

James SW, Ranum LP, Silflow CD, Lefebvre PA (1988) Mutants resistant to anti-microtubule herbicides map to a locus on the uni linkage group in Chlamydomonas reinhardtii. Genetics 118:141-147

James SW, Lefebvre PA (1992) Genetic interactions among Chlamydomonas reinhardtii mutations that confer resistance to anti-microtubule herbicides. Genetics 130:305-314

Silflow CD, Sun X, Haas NA, Foley JW, Lefebvre PA (2011) The Hsp70 and Hsp40 chaperones influence microtubule stability in Chlamydomonas. Genetics 189:1249-60

  • Locus:
  • APM1
  • Chromosome:
  • 17

From Ritsu Kamiya, University of Tokyo, August 1, 2011

This mutant was induced by UV mutagenesis in the 137C background. It lacks several axonemal dyneins.

Yamamoto R, Hirono M, Kamiya R (2010) Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins. J Cell Biol 190:65-71

  • Locus:
  • IDA10 [MOT48]
  • Chromosome:
  • 10

From Ritsu Kamiya, University of Tokyo, August 2011

This mutant was induced by UV mutagenesis in the 137C background. It lacks several axonemal dyneins.

Yamamoto R, Hirono M, Kamiya R (2010) Discrete PIH proteins function in the cytoplasmic preassembly of different subsets of axonemal dyneins. J Cell Biol 190:65-71

  • Locus:
  • IDA10 [MOT48]
  • Chromosome:
  • 10

From Ritsu Kamiya, University of Tokyo, August 2011

This mutant was induced by UV mutagenesis in the 137C background. Unlike other oda2 alleles, this allele has flagella with a truncated gamma heavy chain, hence the name oda2-t. Please see CC-2230 for additional information on the ODA2 locus.

Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R (2008) Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell 7:1136-1145

  • Locus:
  • ODA2 [DHC15]
  • Chromosome:
  • 11

From Ritsu Kamiya, University of Tokyo, August 2011

This mutant was induced by UV mutagenesis in the 137C background. Unlike other oda2 alleles, this allele has flagella with a truncated gamma heavy chain, hence the name oda2-t. Please see CC-2230 for additional information on the ODA2 locus.

Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R (2008) Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell 7:1136-1145

  • Locus:
  • ODA2 [DHC15]
  • Chromosome:
  • 11

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This is a G > A mutation at codon 317 in the ARG9 gene, resulting in a glycine-to-arginine transition at a strictly conserved residue in NAOAT.

Loppes R, Heindricks R (1986) New arginine-requiring mutants in Chlamydomonas reinhardtii. Arch Microbiol 143:348-352

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This is the arg9-2 mutant (see CC-4440) rescued by nuclear transformation with a genomic ARG9 clone.

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, Hamel PP (2009) The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 8:1460-1463

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This strain was generated by crossing arg9-2 (CC-4440) to wild type 137c mt-. This is spore #3 from that cross.

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, Hamel PP (2009) The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 8:1460-1463

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This strain was generated by crossing arg9-2 (CC-4440) to wild type 137c mt-. This is spore #14 from that cross.

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, Hamel PP (2009) The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 8:1460-1463

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This is the arg9-2 mutant (see CC-4440) complemented by chloroplast transformation with pKS-arg9s containing the ARG9 cDNA from Arabidopsis thaliana, with the targeting sequence removed. This strain was derived from spore #1 selected after gamete induction.

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, Hamel PP (2009) The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 8:1460-1463

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This is the arg9-2 mutant (see CC-4440) complemented by chloroplast transformation with pKS-arg9s containing the ARG9 cDNA from Arabidopsis thaliana, with the targeting sequence removed. This strain was derived from spore #2 selected after gamete induction.

Remacle C, Cline S, Boutaffala L, Gabilly S, Larosa V, Barbieri MR, Coosemans N, Hamel PP (2009) The ARG9 gene encodes the plastid-resident N-acetyl ornithine aminotransferase in the green alga Chlamydomonas reinhardtii. Eukaryot Cell 8:1460-1463

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This strain was seletced by crossing arg9-2 mt+ (see CC-4440) to CC-425 arg2 cw15 mt-. This is spore #9 from that cross.

Please see CC-400 for more information on the cw15 mutation.

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Sara Cline and Patrice Hamel, Ohio State University, August 2011

This strain was selected by crossing arg9-2 mt+ (see CC-4440) to CC-425 arg2 cw15 mt-. This is spore #22 from that cross.

Please see CC-400 for more information on the cw15 mutation.

  • Locus:
  • ARG9
  • Chromosome:
  • 13

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min1 mt+ x min2 mt-

  • Locus:
  • MIN1, MIN2
  • Chromosome:

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min1 mt+ x min2 mt-

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min2 mt+ x mlt2 mt-

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

eye1-3 mt- x mlt2 mt+

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min2 mt+ x mlt1 mt-

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min2 mt+ x mlt1-6 mt-