Strains

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

Outcross of pey1 arg7 to 137c mt-

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

pey1-1 mt+ x mlt1-1 mt-

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

eye1-3 mt- x arg7-8 mt+

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

eye1-3 mt- x arg7-8 mt+

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt1-6 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt1-6 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

eye2 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

eye3 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min1 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min1 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min2 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

min2 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

B40 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt1 mt+ x arg7-2 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt1 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt1 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt2 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Joseph Boyd, Carol Diekmann lab, University of Arizona, September 2011

mlt2 mt+ x arg7-8 mt-

  • Locus:
  • ARG7
  • Chromosome:
  • 1

From Tasios Melis, University of California, Berkeley, October 2011

Phenotype: somewhat chlorophyll deficient

This strain has a lower Chl per cell content and higher Chl a / Chl b ratio than corresponding wild type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the tla2 mutant than in the wild type. Biochemical analyses showed that the tla2- FtsY strain was deficient in the Chl a-b light harvesting complex (LHC), and had a Chl antenna size of the photosystems that was only about 65% of that in the wild type.

Kirst H, García-Cerdán JG, Zurbriggen A, Melis A (2012) Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene. Plant Physiol 158:930-45

  • Locus:
  • TLA2
  • Chromosome:

From Tasios Melis, University of California, Berkeley, October 2011

Phenotype: somewhat chlorophyll deficient

This strain has a lower Chl per cell content and higher Chl a / Chl b ratio than corresponding wild type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the tla3 mutant than in the wild type. Biochemical analyses showed that the tla3 strain was deficient in the Chl a-b light harvesting complex (LHC), and had an average Chl antenna size of the photosystems that was only about 45% of that in the wild type.

Kirst H, Garcia-Cerdan JG, Zurbriggen A, Ruehle T, Melis A (2012) Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene. Plant Physiol 160:2251-60

  • Locus:
  • TLA3
  • Chromosome:

From Tasios Melis, University of California, Berkeley, October 2011

Phenotype: somewhat chlorophyll deficient

This strain has a lower Chl per cell content and higher Chl a / Chl b ratio than corresponding wild type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the tla2 mutant than in the wild type. Biochemical analyses showed that the tla3 strain was deficient in the Chl a-b light harvesting complex (LHC), and had a Chl antenna size of the photosystems that was only about 65% of that in the wild type.

Jeong J, Baek K, Kirst H, Melis A, Jin E (2017) Loss of CpSRP54 function leads to a truncated light-harvesting antenna size in Chlamydomonas reinhardtii. Biochim Biophys Acta. 1858:45-55

  • Locus:
  • TLA4
  • Chromosome:

From Tasios Melis, University of California, Berkeley, October 2011

Phenotype: somewhat chlorophyll deficient

This strain has a lower Chl per cell content and higher Chl a / Chl b ratio than corresponding wild type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the tla5 mutant than in the wild type. Biochemical analyses showed that the tla5 strain was deficient in the Chl a-b light harvesting complex (LHC), and had a Chl antenna size of the photosystems that was smaller than that in the wild type.

  • Locus:
  • TLA5
  • Chromosome:

From Tasios Melis, University of California, Berkeley, October 2011

Phenotype: somewhat chlorophyll deficient

This strain was derived upon crosses of tla2- FtsY (cw15) mt+ with a cell wall-containing wild type. The tla2- FtsY CW+ has a lower Chl per cell content and higher Chl a / Chl b ratio than corresponding wild type. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater Pmax values in the mutant than in the wild type. Biochemical analyses showed that the tla2- FtsY CW+ strain was deficient in the Chl a-b light harvesting complex (LHC), and had a smaller Chl antenna size of the photosystems.

Kirst H, García-Cerdán JG, Zurbriggen A, Melis A (2012) Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene. Plant Physiol 158:930-45

  • Locus:
  • TLA2
  • Chromosome:

From Mary Porter, University of Minnesota, December 2011

This pf2 allele was generated by insertion of a NIT1 plasmid (pMN24) into the PF2 gene. Background strain was nit1-305 mt-. Original isolate to Mary Porter from David Mitchell obtained by insertional mutagenesis.

Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57

  • Locus:
  • PF2
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, December 2011

Progeny of backcross to wild-type.

Rupp G, Porter ME (2003) A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product. J Cell Biol 162:47-57

  • Locus:
  • PF2
  • Chromosome:
  • 11

From Mary Porter, University of Minnesota, December 2011

pf14 pf2-4 double mutant transformed with PF2-GFP, isolate 2a.

Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52

  • Locus:
  • PF14 [RSP3], PF2
  • Chromosome:
  • 6,11

From Mary Porter, University of Minnesota, December 2011

bop2-1 mutant transformed with a wild-type copy of the BOP2 gene, isolate c7.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

From Mary Porter, University of Minnesota, December 2011

ida8-1 (59c2) mutant transformed with phage H9, isolate 6C2.

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

From Mary Porter, University of Minnesota, December 2011

T8D9 mutant obtained from Bill Dentler.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22

From Mary Porter, University of Minnesota, December 2011

d1bLIC mutant transformed with a d1bLIC-GFP construct, isolate 13d.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22