Strains

From Mary Porter, University of Minnesota, December 2011

pf18 mutant transformed with a d1b-LIC-GFP construct, isolate A4.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22

From Mary Porter, University of Minnesota, December 2011

CC-3903 strain transformed with a d1bLIC-GFP construct, isolate A4.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22

From Mary Porter, University of Minnesota, December 2011

A54 e18 strain transformed with an antisense construct of the d1bLIC and nit1.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22

From Mary Porter, University of Minnesota, December 2011

A54 e18 strain transformed with an antisense construct of d1bLIC and nit1.

Reck J, Schauer AM, VanderWaal Mills K, Bower R, Tritschler D, Perrone CA, Porter ME (2016) The role of the dynein light intermediate chain in retrograde IFT and flagellar function in Chlamydomonas. Mol Biol Cell. 27(15):2404-22

From Mary Porter, University of Minnesota, December 2011

ida6 strain transformed with a wild-type IDA6 gene, isolate G11.

Austin-Tse C, Halbritter J, Zariwala MA, Gilberti RM, Gee HY, Hellman N, Pathak N, Liu Y, Panizzi JR, Patel-King RS, Tritschler D, Bower R, O'Toole E, Porath JD, Hurd TW, Chaki M, Diaz KA, Kohl S, Lovric S, Hwang DY, Braun DA, Schueler M, Airik R, Otto EA, Leigh MW, Noone PG, Carson JL, Davis SD, Pittman JE, Ferkol TW, Atkinson JJ, Olivier KN, Sagel SD, Dell SD, Rosenfeld M, Milla CE, Loges NT, Omran H, Porter ME, King SM, Knowles MR, Drummond IA, Hildebrandt F (2013) Zebrafish Ciliopathy Screen Plus Human Mutational Analysis Identifies C21orf59 and CCDC65 Defects as Causing Primary Ciliary Dyskinesia. Am J Hum Genet. 93:672-86

From Mary Porter, University of Minnesota, December 2011

ida6 strain transformed with a 3HA tagged IDA6 gene

From Mary Porter, University of Minnesota, December 2011

ida6 strain transformed with a GFP tagged IDA6 gene

From Deqiang Duanmu, Lagarias lab, University of California Davis, February 2010

This strain contains the lcib mutant allele and another suppressor gene mutation that hasnÍt been identified yet. Insertional mutagenesis was performed in CC-1860 pmp1 to get a suppressor that can recover the growth of pmp1 at air level of CO2 (350 ppm). The pmp1-su1 was crossed to CC-4156 ad1-1 to get ad-su1.

Duanmu D, Spalding MH (2011) Insertional suppressors of Chlamydomonas reinhardtii that restore growth of air-dier lcib mutants in low CO2. Photosynthesis Research 109:123-132

From Deqiang Duanmu, Lagarias lab, University of California Davis, February 2010

This strain contains the lcib mutant allele and another suppressor gene mutation that hasn’t been identified yet. Insertional mutagenesis was performed in CC-1860 pmp1 to get this suppressor that can recover the growth of pmp1 at air level of CO2 (350ppm). The pmp1-su4 was crossed to CC-4156 ad1-1 to get ad-su4.

Duanmu D, Spalding MH (2011) Insertional suppressors of Chlamydomonas reinhardtii that restore growth of air-dier lcib mutants in low CO2. Photosynthesis Research 109:123-132

From Deqiang Duanmu, Lagarias lab, University of California Davis, February 2010

This strain contains the lcib mutant allele and another suppressor gene mutation that hasnÍt been identified yet. Insertional mutagenesis was performed in CC-1860 pmp1 to get this suppressor that can recover the growth of pmp1 at air level of CO2 (350ppm). The pmp1-su5 was crossed to CC-4156 ad1-1 to get ad-su5.

Duanmu D, Spalding MH (2011) Insertional suppressors of Chlamydomonas reinhardtii that restore growth of air-dier lcib mutants in low CO2. Photosynthesis Research 109:123-132

From Deqiang Duanmu, Lagarias lab, University of California Davis, February 2010

This strain contains the lcib mutant allele and another suppressor gene mutation that hasnÍt been identified yet. Insertional mutagenesis was performed in CC-4154 ad1 to get this suppressor that can recover the growth of ad1 at air level of CO2 (350ppm). The walless ad-su8 was crossed to CC-620 to get the walled progeny of ad-su8.

Duanmu D, Spalding MH (2011) Insertional suppressors of Chlamydomonas reinhardtii that restore growth of air-dier lcib mutants in low CO2. Photosynthesis Research 109:123-132

From Mary Porter, U of MN, March 2012

ida8-1 strain transformed with 3HA tagged BOP2 gene, isolate D9

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

From Mary Porter, U of MN, March 2012

bop2-1 strain transformed with HA tagged BOP2 gene, isolate F12

Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O'Toole ET, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Mol Biol Cell. 2019 Oct 1;30(21):2659-2680. doi: 10.1091/mbc.E19-07-0367. Epub 2019 Sep 4. PMID: 31483737; PMCID: PMC6761771.

From Dudley Page, Merchant Lab, UCLA, February 2012

The PDAT1 (Phospholipid Diacylglycerol AcylTransferase) gene is disrupted by insertion of a 1.7 kb AphVIII cassette (paromomycin resistance) in exon 12.

PDAT1
Chlamydomonas genome version 4.0 PID: 519119
Augustus 10 model ID: Cre02.g106400
GenBank accession JN815265

Boyle NR, Page MD, Liu B, Blaby IK, Casero D, Kropat J, Cokus SJ, Hong-Hermesdorf A, Shaw J, Karpowicz SJ, Gallaher SD, Johnson S, Benning C, Pellegrini M, Grossman A, Merchant SS (2012) Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas. J Biol Chem 287:15811-25

From Dudley Page, Merchant Lab, UCLA, February 2012

The PDAT1 (Phospholipid Diacylglycerol AcylTransferase) gene is disrupted by insertion of a 1.7 kb AphVIII cassette (paromomycin resistance) in intron 6.

PDAT1
Chlamydomonas genome version 4.0 PID: 519119
Augustus 10 model ID: Cre02.g106400
GenBank accession JN815265

Boyle NR, Page MD, Liu B, Blaby IK, Casero D, Kropat J, Cokus SJ, Hong-Hermesdorf A, Shaw J, Karpowicz SJ, Gallaher SD, Johnson S, Benning C, Pellegrini M, Grossman A, Merchant SS (2012) Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas. J Biol Chem 287:15811-25

From Dudley Page, Merchant Lab, UCLA, February 2012

The NRR1 (Nitrogen Response Regulator) gene is disrupted by insertion of a 1.7 kb AphVIII cassette (paromomycin resistance) in exon 7.

NRR1
Chlamydomonas genome version 4.0 PID: 536284
Augustus 10 model ID: Cre16.g673250

Boyle NR, Page MD, Liu B, Blaby IK, Casero D, Kropat J, Cokus SJ, Hong-Hermesdorf A, Shaw J, Karpowicz SJ, Gallaher SD, Johnson S, Benning C, Pellegrini M, Grossman A, Merchant SS (2012) Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas. J Biol Chem 287:15811-25

From Jean-David Rochaix, University of Geneva, Switzerland, April 2012
The nac2-26 strain was transformed with plasmid Cyc6-Nac2midi containing the paromomycin resistance cassette, nac2-26::Cyc6-Nac2
The target site of Nac2 is the psbD 5ÍUTR; addition of copper to the medium leads to the loss of psbD mRNA and subsequently to the loss of PSII.

aka nac2::cy6proNac2

Surzycki R, Cournac L, Peltier G, Rochaix JD (2007) Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas. Proc Nat Acad Sci USA 104:17548-17553.

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain SP0106 B6 (pf14::pHYDA1-PF14 mt+) was generated by cotransformation of strain CC-1032 (pf14 mt+) with pSI103 and pHYDA1-PF14. This strain contains one copy of the pHYDA1-PF14 plasmid construct. It is not motile in aerobic conditions.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain B6-F (cehc1 pf14::pHYDA1-PF14 mt+) was selected from MMS mutagenesis of strain SP0106 B6 (CC-4507). It has a constitutive swimming phenotype in aerobic conditions. The mutation chc1 affects expression of hydrogenase genes and copper responsive genes.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Single colony isolate of B6-F used for transformation rescue experiments.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain SP0106 C5 (pf14::pHYDA2-PF14 mt+) was generated by cotransformation of strain CC-1032 (pf14 mt+) with pSI103 and pHYDA2-PF14. This strain contains three copies of the pHYDA2-PF14 plasmid construct. It is not motile in aerobic conditions. Expression of the HYDA1, HYDA2, and HYDG genes is upregulated. Transcript levels for PF14 are upregulated, but apparently the protein is not expressed at levels high enough to result in motility.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain C5-4 (pf14::pHYDA2-PF14 mt+) is a spontaneous mutant strain selected from strain SP0106 C5. It has a constitutive swimming phenotype in aerobic conditions. A transposon TCR1 insertion in the 5’UTR sequence in one copy of the pHYDA2-PF14 plasmid construct caused cell motility. Expression of the PF14, HYDA1, HYDA2, and HYDG genes is upregulated.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain SP0106 A6 (pf14::pHYDA1-PF14 mt+) was generated by cotransformation of strain CC-613 (pf14 mt-) with pSI103 and pHYDA1-PF14. This strain contains one copy of the pHYDA1-PF14 plasmid. It is not motile in aerobic conditions.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Xiaoqing Sun, Silfow Lab, U of MN, April 2012

Strain A6-3 (pf14::pHYDA1-PF14 mt+) spontaneous mutant strain selected from A6. It has a constitutive swimming phenotype in aerobic conditions. This strain has a transposon MRC insertion in the pHYDA1 promoter in the construct.

Sun X, Perera S, Haas N, Lefebvre PA, Silflow CD (2013) Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii. Algal Research 2, 341-351

• Locus:
• PF14 [RSP3]
• Chromosome:
• 6

From Kourosh Salehi-Ashtiani, New York University Abu Dhabi, May 2012

From Kourosh Salehi-Ashtiani, New York University Abu Dhabi, May 2012

From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012

Double mutant isolate obtained by the cross of a ccs4-1 arg- mt+ transformant (transformed with pSL18 conferring paromomycin resistance) with ccs5 (T78.15b-) ARG7 mt-, spores selected on TAP + paromomycin.

This ccs4 ccs5 double mutant #5 is a tight photosynthetic mutant.

From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012

Double mutant isolate obtained by the cross of a ccs4-1 arg- mt+ transformant (transformed with pSL18 conferring paromomycin resistance) with ccs5 (T78.15b-) ARG7 mt-, spores selected on TAP + paromomycin.

The photosynthetic deficiency for this ccs4 ccs5 double mutant #9 is different and weaker than #5 (CC-4516).

From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012

Double mutant isolate obtained by the cross of a ccs4-1 arg- mt+ transformant (transformed with pSL18 conferring paromomycin resistance) with ccs5 (T78.15b-) ARG7 mt-, spores selected on TAP + paromomycin.

The photosynthetic deficiency for this ccs4 ccs5 double mutant #11 is different and weaker than #5 (CC-4516).

From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012

The strain was obtained by crossing CC-3720 ccs4-F2D8 mt- with an arg7-8 mt+ strain.

Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802

• Locus:
• ARG7
• Chromosome:
• 1