Strains
CC-4520 ccs4-1 arg7-8 mt-
$30.00
$30.00
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This ccs4-1 arg7-8 strain is a pSL18 transformation control conferring paromomycin resistance used in complementation experiments.
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This ccs4-1 arg7-8 strain was transformed using pSL18+1kb conferring paromomycin resistance. The 1kb fragment contains the wild type genomic DNA of the CCS4 gene from Chlamydomonas.
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2016
This ccs4-1 arg7-8 strain was transformed using pSL18+CCS4(ORF1) conferring paromomycin resistance. ORF1 corresponds to the wild type CCS4 gene cloned from ATG to stop into the pSL18 vector. Gene amplified from wild type genomic DNA from Chlamydomonas.
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This ccs4-1 arg7-8 strain was transformed using pSL18-CCDA conferring paromomycin resistance. CCDA corresponds to the wild type CCDA gene cloned from ATG to stop into the pSL18 vector. Gene amplified from EST CCDA 14C from Chlamy (cDNA).
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This ccs4-1 arg7-8 strain was co-transformed using pSL18 conferring paromomycin resistance and the EST CCDA 14C plasmid containing the cDNA of CCDA from Chlamydomonas.
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This strain is a cross between ccs4-1 arg7-8 mt+ and CC-2677 cw15 nit1-305 mt- 5D.
Gabilly ST, Kropat J, Karamoko M, Page MD, Nakamoto SS, Merchant SS, Hamel PP (2011) A novel component of the disulfide-reducing pathway required for cytochrome c assembly in plastids. Genetics 187:793-802
CC-4527 ccs5 (CCS5) mt-
$30.00
$30.00
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
This ccs5 strain (T78.15b-) was co-transformed with a PCR product containing the paromomycin resistance cassette (PmR) and a PCR product containing the CCS5 gene amplified from genomic DNA.
Gabilly S, Dreyfuss B, Karamoko M, Corvest V, Kropat J, Page MD, Merchant S, Hamel P (2010) CCS5, a thioredoxin-like protein involved in the assembly of plastid c-type cytochromes. J. Biol. Chem. 285:29738–29749
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
Strain expressing HCF164 from Arabidopsis into the chloroplast genome of ccs5 Chlamydomonas mutant strain.
From Stephane Gabilly, Hamel Lab, The Ohio State University, June 2012
Strain expressing HCF164 from Arabidopsis (codon optimized) into the chloroplast genome of ccs5 Chlamydomonas mutant strain.
CC-4530 sup-pf5
$30.00
$30.00
From Mary Porter, U of MN, June 2012
Piperno G, Mead K, Shestak W (1992) The inner dynein arms I2 interact with a "dynein regulatory complex" in Chlamydomonas flagella. J Cell Biol 118:1455-1463
From Mary Porter, U of MN, June 2012
CC-4532 Mets strain 2137 mt-
$30.00
$30.00
From Sabeeha Merchant, UCLA, June 2012
This strain is believed to be from a cross performed by Robert J. Spreitzer and Laurens Mets on WT strains 21gr mt+ from Ruth Sager and 137c mt- from Robert P. Levine. It was given to Sabeeha Merchant as ñ2137î by Laurens Mets.
This strain was believed to be 2137 WT mt+ (same as CC-3269). However, whole genome sequencing revealed that the strain is mt-. The presence of SNPs unique to both parental strains (21gr and 137c) suggest that this mt- strain probably came from the same cross that produced 2137 mt+.
Can be grown in the dark.
Spreitzer RJ, Mets L (1981) Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes. Plant Physiol 67:565-569
From Martin Jonikas (Carnegie) via Pete Lefebvre, U of MN, July 2012
This strain is a haploid progeny from a cross between 4A- (Dent, Haglund, Chin, Kobayashi, Niyogi, Plant Physiology 2005) and D66+ (Schnell and Lefebvre, Genetics 1993). It was selected from the progeny because it has a number of desirable properties: recovers well from cryogenic storage, electroporates well, mates well, grows well in the dark, grows well photoautotrophically, appears to have a functional carbon concentrating mechanism, does not clump much in liquid culture, swims normally and does not adhere much to glass.
This is the background strain used in the Chlamydomonas Library Project (www.chlamylibrary.org).
From Pete Lefebvre, U of MN, July 2012
From Pete Lefebvre, U of MN, July 2012
From Peter Hegemann, Humboldt University, Berlin, July 2012
Phenotype: altered phototaxis
This is the original strain that Bob Smyth isolated in the Ebersold lab. The isolate was derived as a spontaneous cell-wall-less mutant from a single colony of Smyth strain 806. It shows negative phototaxis at all photon irradiances and has some cell-wall defect.
This strain has a weaker cell wall but it is not cell wall deficient. It is relatively stable but has the advantage that it can be lysed in a so-called Yeda Press (also named Parr Bomb) which is much milder that a French Press or Fluidizer. Thus internal membranes can be isolated with higher purity.
Hegemann P, Hegemann U, Foster KW (1988) Reversible bleaching of Chlamydomonas reinhardtii rhodopsin in vivo. Photochem Photobiol 48:123-8
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This strain was generated from a cross of CC-4513 to CC-613. It has a constitutive swimming phenotype in aerobic conditions and a transposon MRC insertion in the pHYDA1 promoter in the construct.
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This is a spontaneous mutant strain selected from CC-4512. It has a constitutive swimming phenotype in aerobic conditions and has an insertion in the pHYDA1 promoter in the construct.
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This strain was generated from a cross of strain CC-4538 to CC-613. It has a constitutive swimming phenotype in aerobic conditions and an insertion in the pHYDA1 promoter in the construct.
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This is a spontaneous mutant selected from CC-4512. It has a constitutive swimming phenotype in aerobic conditions. No insertion in the pHYDA1 promoter in the construct was detected in a Southern blot experiment.
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This strain was generated from a cross of strain CC-4540 to CC-613. It has a constitutive swimming phenotype in aerobic conditions. No insertion in the pHYDA1 promoter in the construct was detected in a Southern blot experiment.
From Xiaoqing Sun, Silflow lab, U of MN, October 2012
This strain is a spontaneous mutant strain selected from strain CC-4507. It has a constitutive swimming phenotype in aerobic conditions. No insertion in the pHYDA1 promoter in the construct was detected in a Southern blot experiment.
From Mary Porter, U of MN, October 2012
This strain is a sup-pf4 mutant transformed with an HA tagged FAP155 construct. Transformant # 14F.
Bower R, Tritschler D, Vanderwaal K, Perrone CA, Mueller J, Fox L, Sale WS, Porter ME (2013) The N-DRC forms a conserved biochemical complex that maintains outer doublet alignment and limits microtubule sliding in motile axonemes. Mol Biol Cell 24:1134-52
CC-4548 cox4::BLE mt+
$30.00
$30.00
From Saul Purton, University College London, November 2012
A respiratory mutant defective in cytochrome c oxidase isolated using a colorimetric screen following insertional mutagenesis using the BLE marker carried on pSP115 (Lumbreras et al. 2001. Plant J 14: 441_447). Subsequent analysis demonstrated that the COX4 gene encoding subunit 4 of the oxidase has been deleted in the mutant.
Lown FJ, Watson AT, Purton S (2001) Chlamydomonas nuclear mutants that fail to assemble respiratory or photosynthetic electron transfer complexes. Biochem Soc Trans 29:452-455
CC-4549 cox90::BLE mt-
$30.00
$30.00
From Saul Purton, University College London, November 2012
A respiratory mutant defective in cytochrome c oxidase isolated using a colorimetric screen following insertional mutagenesis using the BLE marker carried on pSP115 (Lumbreras et al. 2001. Plant J 14: 441_447). Subsequent analysis demonstrated that the COX90 gene encoding a novel of the oxidase has been deleted in the mutant.
Lown FJ, Watson AT, Purton S (2001) Chlamydomonas nuclear mutants that fail to assemble respiratory or photosynthetic electron transfer complexes. Biochem Soc Trans 29:452-455
CC-4550 sco1::BLE mt-
$30.00
$30.00
From Saul Purton, University College London, November 2012
A respiratory mutant defective in cytochrome c oxidase isolated using a colorimetric screen following insertional mutagenesis using the BLE marker carried on pSP115 (Lumbreras et al. 2001. Plant J 14: 441–447). Subsequent analysis demonstrated that the SCO1 gene encoding a copper chaperone involved in assembly of the oxidase has been deleted in the mutant.
Lown FJ, Watson AT, Purton S (2001) Chlamydomonas nuclear mutants that fail to assemble respiratory or photosynthetic electron transfer complexes. Biochem Soc Trans 29:452-455
From Yanjie Liu, Snell lab, UT Southwestern Medical School, December 2012
mt- gamete fusion defective
Liu Y, Tewari R, Ning J, Blagborough AM, Garbom S, Pei J, Grishin NV, Steele RE, Sinden RE, Snell WJ, Billker O (2008) The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes Dev 22:1051-68
CC-4552 hap2::nit mt- [40D4]
$30.00
$30.00
From Yanjie Liu, Snell lab, UT Southwestern Medical School, December 2012
mt- gamete fusion defective
Liu Y, Pei J, Grishin N, Snell WJ (2015) The cytoplasmic domain of the gamete membrane fusion protein HAP2 targets the protein to the fusion site in Chlamydomonas and regulates the fusion reaction. Development. 142:962-71
CC-4553 oda16-1 mt-
$30.00
$30.00
From David Mitchell, SUNY Upstate Medical University, January 2013
Phenotype: impaired motility
This strain was generated by insertional mutagenesis with pMN24 (NIT-1) into nit1-305, cw15. Two backcrosses to 137c were used to create a strain more compatible with other 137c-background strains in crosses. It is defective in outer dynein arm assembly and swims slowly; about 10% of WT dynein levels still assemble.
Ahmed NT, Mitchell DR (2005) ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell 16:5004-5012
Ahmed NT, Gao C, Lucker BF, Cole DG, Mitchell DR (2008) ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. J Cell Biol 183:313-322
CC-4554 oda16-1 mt+
$30.00
$30.00
From David Mitchell, SUNY Upstate Medical University, January 2013
Phenotype: impaired motility
This strain was generated by insertional mutagenesis with pMN24 (NIT-1) into nit1-305, cw15. Two backcrosses to 137c were used to create a strain more compatible with other 137c-background strains in crosses. It is defective in outer dynein arm assembly and swims slowly; about 10% of WT dynein levels still assemble.
Ahmed NT, Mitchell DR (2005) ODA16p, a Chlamydomonas flagellar protein needed for dynein assembly. Mol Biol Cell 16:5004-5012
Ahmed NT, Gao C, Lucker BF, Cole DG, Mitchell DR (2008) ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery. J Cell Biol 183:313-322
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