Tulin and Cross TS-Lethal Collection
ts184 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts184 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts189 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts189 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts191 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts193 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts193 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts197 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts197 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts199 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts199 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts24 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts29 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts29 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts302 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts302 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts303 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts303 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts304 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts304 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts308 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts308 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts309 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts309 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts310 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts310 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts311 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts311 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts313 mt-
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
ts313 mt+
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38