Tulin and Cross TS-Lethal Collection
div16-4 mt- [Cross TL21-33]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div16-4 mt+ [Cross TL21-33]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div17-1 mt- [Cross ts194]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div17-1 mt+ [Cross ts194]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div17-2 mt- [Cross ts307]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div17-2 mt+ [Cross ts307]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-1 mt- [Cross ts188]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-1 mt+ [Cross ts188]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-2 mt- [Cross ts314]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-2 mt+ [Cross ts314]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-3 mt- [Cross TL7-8]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-3 mt+ [Cross TL7-8]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-4 mt- [Cross TL20-75]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-4 mt+ [Cross TL20-75]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-5 mt- [Cross TL19-83]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div19-5 mt+ [Cross TL19-83]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div20-1 mt- [Cross ts120]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div20-1 mt+ [Cross ts120]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div20-2 mt- [Cross TL7-13]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div20-2 mt+ [Cross TL7-13]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div21-1 mt- [Cross ts320]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div21-1 mt+ [Cross ts320]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div21-2 mt- [Cross TL7-27]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div22-1 mt- [Cross TL7-38]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div22-1 mt+ [Cross TL7-38]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div23-1 mt- [Cross tsm34]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div23-1 mt+ [Cross tsm34]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div23-2 mt- [Cross TL7-20]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div23-2 mt+ [Cross TL7-20]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div23-3 mt- [Cross TL?-21]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
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