Tulin and Cross TS-Lethal Collection
div31-2 mt- [Cross TL17-18]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div31-2 mt+ [Cross TL17-18]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div34-1 mt- [Cross ts317]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div34-1 mt+ [Cross ts317]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div35-1 mt- [Cross ts319]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div35-1 mt+ [Cross ts319]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div36-1 mt- [Cross ts321]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div36-1 mt+ [Cross ts321]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div37-1 mt- [Cross ts176]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div37-1 mt+ [Cross ts176]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div37-2 mt- [Cross TL17-17]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div37-2 mt+ [Cross TL17-17]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div38-1 mt- [Cross ts196]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div38-1 mt+ [Cross ts196]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div39-1 mt- [Cross TL21-11]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div39-1 mt+ [Cross TL21-11]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div40-1 mt- [Cross TL21-55]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div40-1 mt+ [Cross TL21-55]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div41-1 mt- [Cross TL21-23]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div41-1 mt+ [Cross TL21-23]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div42-1 mt- [Cross TL21-31]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div42-1 mt+ [Cross TL21-31]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div43-1 mt- [Cross TL23-21]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div43-1 mt+ [Cross TL23-21]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div44-1 mt- [Cross TL23-13]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div44-1 mt+ [Cross TL23-13]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div45-1 mt- [Cross tsm20]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div45-1 mt+ [Cross tsm20]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div46-1 mt- [Cross TL20-93]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
div46-1 mt+ [Cross TL20-93]
$30.00
$30.00
From Fred Cross, The Rockefeller University, May 2016
This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.
For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.
Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38
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