Tulin and Cross TS-Lethal Collection

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV47 [GIF3_GRF1-interacting_factor_3]
  • Chromosome:
  • 1

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV47 [GIF3_GRF1-interacting_factor_3]
  • Chromosome:
  • 1

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV48 [CDKB]
  • Chromosome:
  • 8

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV48 [CDKB]
  • Chromosome:
  • 8

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV49 [SPC97_98]
  • Chromosome:
  • 12

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV49 [SPC97_98]
  • Chromosome:
  • 12

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV50 [RFC]
  • Chromosome:
  • 12

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV50 [RFC]
  • Chromosome:
  • 12

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV51 [TFC-B/CPN2]
  • Chromosome:
  • 15

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV51 [TFC-B/CPN2]
  • Chromosome:
  • 15

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV52 [THY-2_thymidylate_synthase_2]
  • Chromosome:
  • 17

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV52 [THY-2_thymidylate_synthase_2]
  • Chromosome:
  • 17

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV53 [3 clean hits on chr_17 5.5-6.5; no annotations; cand. gene picked at random]
  • Chromosome:
  • 17

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV53 [3 clean hits on chr_17 5.5-6.5; no annotations; cand. gene picked at random]
  • Chromosome:
  • 17

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV55 [kinase]
  • Chromosome:
  • 5

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV55 [picked one candidate on chr5 at random. chr5 ~3 fnl hits uniform; no obvious candidate among them; rest of genome clean]
  • Chromosome:
  • 5

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV55 [picked one candidate on chr5 at random. chr5 ~3 fnl hits uniform; no obvious candidate among them; rest of genome clean]
  • Chromosome:
  • 5

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV57 [Only hit, only SNP. Plausible Augmin-4 homolog, microtubule nucleation]
  • Chromosome:
  • 6

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV57 [Only hit, only SNP. Plausible Augmin-4 homolog, microtubule nucleation]
  • Chromosome:
  • 6

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV60 [P450 reductase]
  • Chromosome:
  • 1

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV61 [APY2]
  • Chromosome:
  • 6

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV64 [CTP_synthase_family_protein]
  • Chromosome:
  • 9

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV64 [CTP_synthase_family_protein]
  • Chromosome:
  • 9

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV64 [CTP_synthase_family_protein]
  • Chromosome:
  • 9

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV64 [CTP_synthase_family_protein]
  • Chromosome:
  • 9

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV73
  • Chromosome:
  • 5, 16

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV73
  • Chromosome:
  • 5, 16

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV73
  • Chromosome:
  • 5, 16

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV73
  • Chromosome:
  • 5, 16

From Fred Cross, The Rockefeller University, May 2016

This collection of ts-lethal mutations was generated by UV mutagenesis. The location and probable identity of the causative mutation was determined by bulked segregant sequence analysis (Tulin and Cross 2014). Information is provided identifying the most likely causative mutation. All strains were backcrossed at least once to WT. It is highly recommended that the co-segregation of the indicated mutation (for example by allele-specific PCR) and ts-lethality be confirmed as a first step, before carrying out additional experiments. As noted in the publication, the map location of the causative mutation is highly likely to be correct. Identification of the causative mutation itself involves less certain inference.

For allele-specific PCR, we have had a very high success rate with the competitive amplification assay using oligos designed according to the principles described in Onishi, Pringle and Cross 2015.

Supplemental Information

Tulin F, Cross FR (2014) A microbial avenue to cell cycle control in the plant superkingdom. Plant Cell. 26(10): 4019-38

  • Locus:
  • DIV73 [ATPI4K_ALPHA_Phosphatidylinositol_3-_and_4-kinase]
  • Chromosome:
  • 5